Probiotic compositions and methods of use

Abstract

A probiotic composition and methods for the mitigation, inhibition, and/or exclusion of microorganisms.

What is claimed is: 
     
         1 . A probiotic composition comprising:
 at least one biosurfactant-producing microorganism;   at least one antiadhesion-producing microorganism;
 wherein the at least one biosurfactant-producing microorganism and the at least one antiadhesion-producing microorganism mitigate pathogenic biofilm when applied on a surface. 
   
     
     
         2 . The probiotic composition of  claim 1 , wherein the at least one biosurfactant-producing microorganism is selected from the group consisting of  Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus paracasei, Lactobacillus fermentum, Lactobacillus lactis, Lactobacillus reuterii, Lactobacillus rhamnoses, Lactobacillus bulgaricus, Lactobacillus plantarum, Lactobacillus salivarius, Lactobacillus delbrueckii, Lactobacillus helveticus, Lactobacillus brevis, Lactobacillus sakei, Lactobacillus johnsonii, Bifidobacterium longum, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium adolecentis, Bifidobacterium lactis, Bidobacterium animalis, Enterococcus faecium , and combinations thereof. 
     
     
         3 . The probiotic composition of  claim 1 , wherein the at least one antiadhesion-producing microorganism is selected from the group consisting of  Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus paracasei, Lactobacillus fermentum, Lactobacillus lactis, Lactobacillus reuterii, Lactobacillus rhamnoses, Lactobacillus bulgaricus, Lactobacillus plantarum, Lactobacillus salivarius, Lactobacillus delbrueckii, Lactobacillus helveticus, Lactobacillus brevis, Lactobacillus sakei, Lactobacillus johnsonii, Bifidobacterium longum, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium adolecentis, Bifidobacterium lactis, Bidobacterium animalis, Enterococcus faecium , and combinations thereof. 
     
     
         4 . The probiotic composition of  claim 1 , wherein the at least one pathogen is selected from the group consisting of  Salmonella.    
     
     
         5 . The probiotic composition of  claim 1 , wherein the surface is a food product contact surface. 
     
     
         6 . The probiotic composition of  claim 1 , wherein the biosurfactant-producing microorganism is present in an amount of from 10 3  to 10 15  colony forming units per gram of the probiotic composition. 
     
     
         7 . The probiotic composition of  claim 1 , wherein the antiadhesion-producing microorganism is present in an amount of from 10 3  to 10 15  colony forming units per gram of the probiotic composition. 
     
     
         8 . The probiotic composition of  claim 1 , comprising a biofilm-producing microorganism. 
     
     
         9 . The probiotic composition of  claim 8 , wherein the biofilm-producing microorganism is present in an amount of 10 3  to 10 15  colony forming units per gram of the probiotic composition. 
     
     
         10 . The probiotic composition of  claim 1 , wherein the at least one biosurfactant-producing microorganism is certified as generally recognized as safe. 
     
     
         11 . The probiotic composition of  claim 1 , wherein the at least one antiadhesion-producing microorganism is certified generally recognized as safe. 
     
     
         12 . The probiotic composition of  claim 1 , wherein the probiotic composition is in aerosol form. 
     
     
         13 . A method of treating a surface of a food processing facility to inhibit the growth of a pathogen thereon, the method comprising the step of:
 disposing a probiotic composition onto the surface of the food processing facility, wherein the probiotic composition comprises at least one biosurfactant-producing microorganism and at least one antiadhesion-producing microorganism, wherein the at least one biosurfactant-producing microorganism and at least one antiadhesion-producing microorganism remove pathogenic biofilm from the surface.   
     
     
         14 . The method of  claim 13 , wherein the biosurfactant-producing microorganism is selected from the group consisting of  Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus paracasei, Lactobacillus fermentum, Lactobacillus lactis, Lactobacillus reuterii, Lactobacillus rhamnoses, Lactobacillus bulgaricus, Lactobacillus plantarum, Lactobacillus salivarius, Lactobacillus delbrueckii, Lactobacillus helveticus, Lactobacillus brevis, Lactobacillus sakei, Lactobacillus johnsonii, Bifidobacterium longum, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium adolecentis, Bifidobacterium lactis, Bidobacterium animalis, Enterococcus faecium , and combinations thereof. 
     
     
         15 . The method of  claim 13 , wherein the at least one antiadherence-producing microorganism is selected from the group consisting of  Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus paracasei, Lactobacillus fermentum, Lactobacillus lactis, Lactobacillus reuterii, Lactobacillus rhamnoses, Lactobacillus bulgaricus, Lactobacillus plantarum, Lactobacillus salivarius, Lactobacillus delbrueckii, Lactobacillus helveticus, Lactobacillus brevis, Lactobacillus sakei, Lactobacillus johnsonii, Bifidobacterium longum, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium adolecentis, Bifidobacterium lactis, Bidobacterium animalis, Enterococcus faecium , and combinations thereof. 
     
     
         16 . The method of  claim 13 , wherein the at least one pathogen is selected from the group consisting of  Salmonella.    
     
     
         17 . The method of  claim 13 , wherein the surface of the food processing facility is a food product contact surface. 
     
     
         18 . The method of  claim 1 , wherein the biosurfactant-producing microorganism is present in an amount of from 10 3  to 10 15  colony forming units per gram of the probiotic composition and wherein the antiadhesion-producing microorganism is present in an amount of from in an amount of 10 3  to 10 15  colony forming units per gram of the probiotic composition. 
     
     
         19 . The method of  claim 13 , wherein the at least one biosurfactant-producing microorganism is certified generally recognized as safe (GRAS) and, wherein the at least one antiadhesion-producing microorganism is generally recognized as safe (G RAS). 
     
     
         20 . A method for mitigating pathogenic biofilm and inhibiting pathogen growth on a food product contact surface, the method comprising the step of applying a probiotic composition comprising at least one biosurfactant-producing microorganism and at least one antiadhesion-producing microorganism to the food product contact surface.