Preparative separation and purification device and preparative separation and purification method

Abstract

A target component is collected using a preparative separation and purification device having a holder for holding a trap column in which the target component has been captured, a liquid feeder for feeding a first solvent having compatibility with the water remaining in the trap column and a second solvent having low compatibility with water and high compatibility with the first solvent into the trap column, a flow-path switch for connecting the exit end of the trap column to a waste liquid flow path and a collection flow path, and a control unit for controlling the flow-path switch so that solution including water flows into the waste liquid flow path.

1 - 2 . (canceled) 
     
     
         3 . A preparative separation and purification device for allowing a solution including a target component and a trapping solvent to flow through a trap column to trap the target component in the trap column and allowing a solvent different from the trapping solvent to flow through the trap column to collect the target component trapped in the trap column, comprising:
 a) a holding means for holding the trap column so that an entrance end of the trap column is directed downward and an exit end thereof is directed upward;   b) a liquid feeding means selectively connected to a first solvent source storing a first solvent having a lower boiling point than the trapping solvent and having compatibility with the trapping solvent remaining in the trap column and a second solvent source storing a second solvent having a lower boiling point than the trapping solvent, having a higher specific gravity than the trapping solvent and the first solvent, having high compatibility with the first solvent, and having low compatibility with the trapping solvent while the target component is trapped in the trap column held by the holding means and feeding any one of the first solvent and the second solvent to the entrance end of the trap column;   c) a liquid feed control means for connecting the liquid feeding means to the second solvent source so that the second solvent flows through the trap column after connecting the liquid feeding means to the first solvent source for a predetermined time so that the first solvent flows through the trap column while the target component is trapped in the trap column held by the holding means and the trapping solvent remains in the trap column;   d) a flow-path switching means for selectively connecting the exit end of the trap column to any one of a waste liquid flow path and a collection flow path; and   e) a flow-path control means for controlling the flow-path switching means so that the exit end is connected to the waste liquid flow path when a solution including the trapping solvent is discharged from the exit end of the trap column, the exit end is connected to the collection flow path when a solution including the second solvent is discharged from the exit end of the trap column, and a connection destination of the exit end is switched from the waste liquid flow path to the collection flow path at a predetermined timing until a solution including the second solvent starts to be discharged after a solution including the first solvent is discharged from the exit end of the trap column.   
     
     
         4 . A preparative separation and purification method of allowing a solution including a target component and a trapping solvent to flow through a trap column to trap the target component in the trap column and allowing a solvent different from the trapping solvent to flow through the trap column to collect the target component trapped in the trap column, comprising:
 a) feeding a first solvent having a lower boiling point than the trapping solvent and having compatibility with the trapping solvent remaining in the trap column to an entrance end of the trap column for a predetermined time while the target component is trapped in the trap column held so that the entrance end of the trap column is directed downward and an exit end thereof is directed upward;   b) feeding a second solvent having a lower boiling point than the trapping solvent, a higher specific gravity than the trapping solvent and the first solvent, having high compatibility with the first solvent, and having low compatibility with the trapping solvent to the entrance end of the trap column; and   c) allowing a solution including the trapping solvent to flow through a waste liquid flow path when the solution is discharged from the exit end of the trap column, allowing a solution including the second solvent to flow through a collection flow path when the solution is discharged from the exit end of the trap column, and switching the flow path of the solution discharged from the exit end from the waste liquid flow path to the collection flow path at a predetermined timing until the solution including the second solvent starts to be discharged after a solution including the first solvent is discharged from the exit end of the trap column.