Nitrogen-based, low-temperature atmospheric pressue plasma for treating muscle damage

Abstract

The present invention relates to a method for producing a nitrogen-based non-thermal atmospheric pressure plasma for treating damaged muscles or promoting regeneration of damaged muscles, and to a method for treating damaged muscle or promoting regeneration of damaged muscles using the atmospheric pressure plasma. The nitrogen-based non-thermal atmospheric pressure plasma according to the present invention can effectively induce muscle cell activation, migration of muscle cells to damaged sites, muscle cell proliferation, and muscle cell differentiation, without surgical operation or during a treatment procedure after surgical operation, and thus can be advantageously used as a novel therapeutic agent and therapeutic method against muscle damage and muscle damage-associated diseases.

What is claimed is: 
     
         1 . A method for producing a nitrogen-based non-thermal atmospheric pressure plasma for treating damaged muscles or promoting regeneration of damaged muscles, the method comprising the steps of:
 introducing nitrogen gas as a carrier gas; and   applying a discharge initiation voltage of 5 to 10 kV to the introduced nitrogen gas at a frequency of 5 to 20 kHz, thereby producing a nitrogen-based non-thermal atmospheric pressure plasma.   
     
     
         2 . The method of  claim 1 , wherein the treating of the damaged muscles or the regeneration of the damaged muscles is achieved by one or more selected from the group consisting of muscle cell activation, migration of muscle cells to a damaged site, muscle cell proliferation, and muscle cell differentiation. 
     
     
         3 . The method of  claim 1 , wherein the treating of the damaged muscles is muscle repair. 
     
     
         4 . A nitrogen-based non-thermal atmospheric pressure plasma for treating damaged muscles or promoting regeneration of damaged muscles, produced by the method of  claim 1 . 
     
     
         5 . A method for treating damaged muscles or promoting regeneration of damaged muscles, the method comprising a step of treating a damaged muscle site with a nitrogen-based non-thermal atmospheric pressure plasma for treating damaged muscles or promoting regeneration of damaged muscles, produced by the method of  claim 1 . 
     
     
         6 . The method of  claim 5 , wherein the nitrogen-based non-thermal atmospheric pressure plasma is discharged at 2 to 10 L/minute. 
     
     
         7 . The method of  claim 5 , wherein the damaged muscle site is treated with the nitrogen-based non-thermal atmospheric pressure plasma at a distance of 0.3 to 5 cm. 
     
     
         8 . The method of  claim 5 , wherein treatment with the nitrogen-based non-thermal atmospheric pressure plasma is performed for 10 to 120 seconds once a day. 
     
     
         9 . A pharmaceutical composition for treating muscle damage-associated diseases, comprising a nitrogen-based non-thermal atmospheric pressure plasma produced by the method of  claim 1 . 
     
     
         10 . A method of promoting migration, proliferation or differentiation of muscle cells, the method comprising a step of treating the muscle cells in vitro with a nitrogen-based non-thermal atmospheric pressure plasma for treating damaged muscles or promoting regeneration of damaged muscles, produced by the method of  claim 1 . 
     
     
         11 . The method of  claim 10 , wherein the muscle cells are cultured in growth medium.