Composition for treating diabetes and dyslipidemia obtained from the extract of costus pictus d.don plant and a method of preparing the same

Abstract

The disclosure provides a medicinal composition for treating diabetes and dyslipidemia obtained from the extract of Costus pictus D. Don. The extract of Costus pictus D. Don includes hydroxycinnamic acid derivatives and flavonol mono-, di- and triglycosides along with small amounts of free flavonols. The extract of Costus pictus D. Don has low levels of oxalic acid and/or oxalates. The hydroxycinnamic acid derivatives include caffeic acid, p-coumaric acid, ferulic acid and sinapic acid and flavonol mono-, di- and triglycosides which include quercetin, kaempferol and isorhamnetin with sugars glucose, galactose and rhamnose. Disclosure also provides a method of preparing the extract of Costus pictus D. Don of the same. The disclosure also provides a method of treating mammals including human beings for medical conditions such as diabetes; dyslipidemia and related conditions; for increasing anti oxidant potential; for the regeneration of pancreatic beta cells and increasing the insulin secretogogue effect.

What is claimed is: 
     
         1 . A composition comprising an extract of  Costus pictus  D. Don, the extract comprising hydroxycinnamic acid derivatives and flavonols; and, wherein a total of oxalic acid and oxalates content in the extract is less than 15%. 
     
     
         2 . The composition of  claim 1 , wherein the flavonols comprise mono-flavonols, di-flavonols, flavonol triglycosides and flavonols in free form. 
     
     
         3 . The composition of  claim 1 , wherein the hydroxycinnamic acid derivatives comprise caffeic acid, p-coumaric acid, ferulic acid and sinapic acid. 
     
     
         4 . The composition of  claim 1 , wherein the flavonols comprise quercetin, kaempferol, isorhamnetin, glucose derivatives of quercetin, glucose derivatives of kaempferol, glucose derivatives of isorhamnetin, galactose derivatives of quercetin, galactose derivatives of kaempferol, galactose derivatives of isorhamnetin, rhamnose derivatives of quercetin, rhamnose derivatives of kaempferol, and rhamnose derivatives of isorhamnetin. 
     
     
         5 . A dosage form of the composition of  claim 1 , the dosage form selected from the group consisting of capsule, tablet, granule, sachet, powder, paste, ointment, infusion, injection, ampoule, solution, suspension, emulsion, pill, sustained release formulation and combinations thereof. 
     
     
         6 . A dosage form of the composition of  claim 1  further comprising fillers, the fillers selected from the group consisting of lactose, spray dried lactose, starch, dibasic calcium phosphate, tribasic calcium phosphate, microcrystalline cellulose, hydroxy propyl methyl cellulose, and calcium carbonate. 
     
     
         7 . A dosage form comprising a dosage in the range of about 500 mg/day to 2000 mg/day of composition for administration to a human subject. 
     
     
         8 . The composition of  claim 1 , wherein the extract of  Costus pictus  D. Don is selected from the group consisting of
 a) a methanol extract of  Costus pictus  D. Don comprising about 10% of oxalic acid;   b) a methanol extract of  Costus pictus  D. Don comprising about 5% of oxalic acid;   c) an ethyl acetate extract of  Costus pictus  D. Don comprising about 2.5% of oxalic acid; and,   d) an ethyl acetate extract of  Costus pictus  D. Don comprising about 1% of oxalic acid.   
     
     
         9 . A composition comprising an extract of  Costus pictus  D. Don, the extract comprising about 10% of oxalic acid, the extract prepared by a method comprising:
 a) cleaning a starting material of  Costus pictus  D. Don;   b) cutting the product of step (a);   c) extracting the product of step (b) with 90% methanol;   d) refluxing the product of step (c) for at the boiling temperature (60-70° C.) of methanol to obtain a residue and a supernatant;   e) filtering to separate the residue and the supernatant of step (d);   concentrating the supernatant of step (e) to obtain a concentrated methanol extract;   g) drying the concentrated methanol extract of step (f) to obtain a powder of the methanol extract;   h) mixing the powder of step (g) with water;   i) heating the product of step (h) to obtain a residue and a supernatant;   j) separating the residue from the supernatant;   k) drying the residue to obtain a dry powder comprising about 10% of oxalic acid.   
     
     
         10 . A composition comprising an extract of  Costus pictus  D. Don, the extract comprising about 5% of oxalic acid, the extract prepared by a method comprising:
 a1) mixing the dry powder of step (k) of  claim 9  with methanol and silica gel to obtain a mixture;   a2) evaporating methanol from the mixture of step a1) to obtain a dry powder;   a3) loading the dry powder of step a2) onto a wet packed silica gel column to obtain an extract loaded silica gel column;   a4) eluting the extract loaded silica gel column of step a3) with hexane to obtain a silica gel column after hexane elution;   a5) eluting the silica gel column eluted with hexane of step a4) with methanol and obtaining a methanol eluate fraction;   a6) concentrating the methanol eluate fraction to form a concentrated extract;   a7) drying the concentrated extract of step a6) to obtain a dry powder comprising about 5% oxalic acid, and wherein the wet packed silica gel comprises silica gel and hexane.   
     
     
         11 . A composition comprising an extract of  Costus pictus  D. Don, the extract comprising about 2.5% of oxalic acid, the extract prepared by a method comprising:
 b1) dissolving the dry powder of step k) of  claim 9  in water to obtain a mixture;   b2) extracting the mixture of step b1) with ethyl acetate to obtain an aqueous phase and an ethyl acetate phase;   b3) concentrating the ethyl acetate phase of step b2) to obtain a concentrated ethyl acetate extract;   b4) drying the ethyl acetate extract under vacuum at above 500 mm of mercury to form a dry powder of the ethyl acetate extract comprising about 2.5% of oxalic acid.   
     
     
         12 . A composition comprising an extract of  Costus pictus  D. Don, the extract comprising about 2.5% of oxalic acid, the extract prepared by a method comprising:
 c1) mixing the dry powder of step b4) of  claim 11  with methanol and silica gel to obtain a mixture;   c2) drying the mixture of step c1) to obtain a dry powder:   c3) loading the dry powder of step c2) onto a wet packed silica gel column to obtain an extract loaded silica gel column, wherein the wet packed silica gel column comprises silica gel column in hexane;   c4) eluting the extract loaded silica gel column of step c3) with hexane to obtain a silica gel column after hexane elution;   c5) eluting the silica gel column eluted with hexane of step c4) with methanol and obtaining a methanol eluate fraction;   c6) concentrating the methanol eluate fraction of step c5) to form a concentrated extract;   c7) drying the concentrated extract of step c6) to obtain a dry powder comprising about 1% oxalic acid.