Immature platelet enumeration systems and methods

Abstract

Embodiments of the present invention encompass automated systems and methods for analyzing immature platelet parameters in an individual based on a biological sample obtained from blood of the individual. Exemplary techniques involve correlating aspects of direct current (DC) impedance, radiofrequency (RF) conductivity, and/or light measurement data obtained from the biological sample with an evaluation of immature platelet conditions in the individual.

1 . A hematology system for determining an immature platelet status in a biological sample, the system comprising:
 a volume conductivity scatter module configured to determine an immature platelet event and a combined blood component event of the biological sample;   a complete blood count module configured to determine a blood cell count and a total platelet count of the biological sample,   a data processing module configured to determine the immature platelet status based on a ratio of the immature platelet event to the combined blood component event, wherein the data processing module is configured to determine the immature platelet status based on a multiplication product of a first factor and a second factor,   wherein the first factor is a ratio of the blood cell count to the total platelet count from the complete blood count module, and wherein the second factor is the ratio of the immature platelet event to the combined blood component event,   wherein the blood cell count is a sum of the red blood cell count, the nucleated red blood cell, and the white blood cell counts, and   wherein the combined blood component event is a sum of red blood cell events, nucleated red blood cell events, and white blood cell events.   
     
     
         2 . (canceled) 
     
     
         3 . The system according to  claim 1 , wherein the immature platelet status comprises an estimation of immature platelet count or an estimation of immature platelet percentage. 
     
     
         4 . The system according to  claim 1 , wherein the combined blood component event is a total platelet event of the biological sample. 
     
     
         5 .- 8 . (canceled) 
     
     
         9 . The system according to  claim 1 , wherein the volume conductivity scatter module is configured to determine the immature platelet event based on a light measurement comprising a member selected from the group consisting of a lower angle light scatter (LALS) measurement, a lower median angle light scatter (LMALS) measurement, an upper median angle light scatter (UMALS) measurement, and an axial light loss (ALL) measurement. 
     
     
         10 . The system according to  claim 9 , wherein the light measurement is the lower angle light scatter (LALS) measurement, and the first module is configured to determine the immature platelet event when a logLALS value is greater than about 200. 
     
     
         11 . The system according to  claim 9 , wherein the light measurement is the lower median angle light scatter (LMALS) measurement, and the first module is configured to determine the immature platelet event when a logLMALS value is greater than about 100. 
     
     
         12 . The system according to  claim 9 , wherein the light measurement comprises the upper median angle light scatter (UMALS) measurement and the lower median angle light scatter (LMALS) measurement, wherein a median angle light scatter (MALS) is sum of the UMALS and LMALS, and the first module is configured to determine the immature platelet event when a log MALS value is greater than about 100. 
     
     
         13 . The system according to  claim 9 , wherein the light measurement is the upper median angle light scatter (UMALS) measurement, and the first module is configured to determine the immature platelet event when a logUMALS value is greater than about 100. 
     
     
         14 . The system according to  claim 9 , wherein the light measurement is the axial light loss (ALL) measurement, and the first module is configured to determine the immature platelet event when a logALL value is greater than about 140. 
     
     
         15 . An automated method for determining an immature platelet status in a biological sample, the method comprising:
 accessing a data profile concerning the biological sample, the data profile based on assay results obtained from a particle analysis system analyzing the biological sample;   determining an immature platelet event and a combined blood component event of the biological sample based on the data profile by executing, with a processor, a storage medium comprising a computer application;   determining the immature platelet status based on a ratio of the immature platelet event to the combined blood component event;   determining a blood cell count and a total platelet count of the biological sample,   determining the immature platelet status based on a multiplication product of a first factor and a second factor,   wherein the first factor is a ratio of the blood cell count to the total platelet count and the second factor is the ratio of the immature platelet event to the combined blood component event,   wherein the blood cell count is a sum of red blood cell count, nucleated red blood cell count, and white blood cell count, and   wherein the combined blood component event is a sum of red blood cell events, nucleated red blood cell event, and white blood cell events.   
     
     
         16 . The method according to  claim 15 , wherein the data profile comprises volume conductivity scatter (VCS) data for the biological sample. 
     
     
         17 . The method according to  claim 15 , wherein the immature platelet status comprises an estimation of immature platelet count or an estimation of immature platelet percentage. 
     
     
         18 . The method according to  claim 15 , further comprising determining a treatment regimen for an individual from whom the biological sample was obtained, based on the immature platelet status. 
     
     
         19 .- 22 . (canceled) 
     
     
         23 . The method according to  claim 15 , wherein the blood cell count and the total platelet count are based on complete blood count (CBC) data obtained for the biological sample. 
     
     
         24 . The method according to  claim 15 , wherein the execution of the storage medium comprising the computer application causes the processor to determine the immature platelet event based on a light measurement comprising a member selected from the group consisting of a lower angle light scatter (LALS) measurement, a lower median angle light scatter (LMALS) measurement, an upper median angle light scatter (UMALS) measurement, and an axial light loss (ALL) measurement. 
     
     
         25 . The method according to  claim 24 , wherein the light measurement is the lower angle light scatter (LALS) measurement, and the execution of the storage medium comprising the computer application causes the processor to determine the immature platelet event when a logLALS value is greater than about 200. 
     
     
         26 . The method according to  claim 24 , wherein the light measurement is the lower median angle light scatter (LMALS) measurement, and the execution of the storage medium comprising the computer application causes the processor to determine the immature platelet event when a logLMALS value is greater than about 100. 
     
     
         27 . The method according to  claim 24 , wherein the light measurement comprises the upper median angle light scatter (UMALS) measurement and the lower median angle light scatter (LMALS) measurement, wherein a median angle light scatter (MALS) is sum of the UMALS and LMALS, and the execution of the storage medium comprising the computer application causes the processor to determine the immature platelet event when a log MALS value is greater than about 100. 
     
     
         28 . The method according to  claim 24 , wherein the light measurement is the upper median angle light scatter (UMALS) measurement, and the execution of the storage medium comprising the computer application causes the processor to determine the immature platelet event when a logUMALS value is greater than about 100. 
     
     
         29 . The method according to  claim 24 , wherein the light measurement is the axial light loss (ALL) measurement, and the execution of the storage medium comprising the computer application causes the processor to determine the immature platelet event when a logALL value is greater than about 140. 
     
     
         30 - 61 . (canceled)