Isolated Novel Nucleic Acid and Protein Molecules from Soybeans and Methods of Using Those Molecules to Generate Transgenic Plants With Enhanced Agronomic Traits

Abstract

This disclosure provides purified nucleic acids and polypeptides. Also provided are transgenic plants, seeds, and plant cells containing DNA for expression of the proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants, methods of making such plants and methods of making agricultural commodity including seeds and hybrid seeds from such plants.

1 . A transgenic plant cell comprising a recombinant DNA construct comprising a promoter that is functional in plant cells and that is operably linked to a polynucleotide that
 a) encodes a polypeptide having an amino acid sequence sharing at least 90% sequence identity with a reference sequence selected from the group consisting of SEQ ID NOs:91968-183370; or   b) is transcribed into a non-coding RNA molecule that is capable of binding to an RNA encoding said polypeptide and suppresses said polypeptide.   
     
     
         2 . (canceled) 
     
     
         3 . (canceled) 
     
     
         4 . The transgenic plant cell according to  claim 1 ,
 wherein said recombinant DNA construct is stably integrated into a chromosome in a plant cell nucleus.   
     
     
         5 . The transgenic plant cell according to  claim 1 , wherein said recombinant DNA construct provides for an enhanced trait as compared to control plants which do not contain said recombinant DNA construct; and wherein said enhanced trait is enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein or enhanced seed oil. 
     
     
         6 . The transgenic plant cell according to  claim 5 , further comprising DNA expressing a protein that provides tolerance from exposure to an herbicide comprising an agent applied at levels that are lethal to a wild type of said plant cell nucleus. 
     
     
         7 . The transgenic plant cell according to  claim 6  wherein the agent of said herbicide is a glyphosate, dicamba, or glufosinate compound. 
     
     
         8 . A transgenic plant or seed comprising a plurality of transgenic plant cells of  claim 5 . 
     
     
         9 . The transgenic plant or seed according to  claim 8  which is homozygous for said recombinant DNA construct. 
     
     
         10 . (canceled) 
     
     
         11 . The transgenic plant or seed according to  claim 8 , wherein said plant or seed is a corn, soybean, cotton, canola, alfalfa, wheat, rice, sugarcane, or sugar beet. 
     
     
         12 . (canceled) 
     
     
         13 . A method for manufacturing non-natural, transgenic seed that can be used to produce a crop of transgenic plants with an enhanced trait said method comprising:
 (a) screening a population of plants for a recombinant DNA construct comprising a promoter that is functional in plant cells and that is operably linked to DNA from a plant, bacteria or yeast a polynucleotide that
 i) encodes a protein polypeptide having an amino acid sequence having sharing at least 90% sequence identity to a reference sequence selected from the group consisting of SEQ ID NOs:91968-183370; or 
 ii) is transcribed into a non-coding RNA molecule that binds to an RNA encoding said polypeptide and suppresses the level of said polypeptide; 
   (b) selecting from said population one or more plants that comprise said recombinant DNA construct, and   (c) collecting seed from selected plant from step b.   
     
     
         14 . The method according to  claim 13 , wherein said method for manufacturing said transgenic seed further comprises
 analyzing tissue of said selected plant to determine the expression or suppression of said polypeptide.   
     
     
         15 . The method according to  claim 14 , wherein said seed is corn, soybean, cotton, canola, alfalfa, wheat, rice, sugarcane, or sugar beet seed. 
     
     
         16 . (canceled) 
     
     
         17 . (canceled) 
     
     
         18 . (canceled) 
     
     
         19 . The transgenic plant cell according to  claim 1 , wherein said polypeptide has an amino acid sequence sharing at least 95% sequence identity with a reference sequence selected from the group consisting of SEQ ID NOs:91968-183370 when said amino acid sequence is aligned to said reference sequence. 
     
     
         20 . The transgenic plant cell according to  claim 19 , wherein said polypeptide has an amino acid sequence sharing at least 98% sequence identity with a reference sequence selected from the group consisting of SEQ ID NOs:91968-183370. 
     
     
         21 . The transgenic plant cell according to  claim 20 , wherein said polypeptide has an amino acid sequence sharing at least 99% sequence identity with a reference sequence selected from the group consisting of SEQ ID NOs:91968-183370. 
     
     
         22 . The transgenic plant cell according to  claim 19 , wherein said polypeptide has an amino acid sequence sharing at least 99.5% sequence identity with a reference sequence selected from the group consisting of SEQ ID NOs:91968-183370. 
     
     
         23 . The transgenic plant cell according to  claim 19 , wherein said polypeptide has an amino acid sequence selected from the group consisting of SEQ ID NOs:91968-183370. 
     
     
         24 . The method according to  claim 13 , wherein said polypeptide has an amino acid sequence of a reference sequence sharing at least 98% sequence identity over 98% of the length of a reference sequence selected from the group consisting of SEQ ID NOs:91968-183370. 
     
     
         25 . The method according to  claim 24 , wherein said polypeptide has an amino acid sequence selected from the group consisting of SEQ ID NOs:91968-183370. 
     
     
         26 . A transgenic plant or seed comprising a recombinant DNA construct, wherein said recombinant DNA construct comprises:
 (a) an exogenous promoter region which functions in a plant cell; which is linked to   (b) a structural nucleic acid molecule comprising a nucleic acid sequence, wherein said nucleic acid sequence exhibits 95% or greater identity to a reference sequence selected from the group consisting of SEQ ID NOs:1-91967.   
     
     
         27 . The transgenic plant or seed according to  claim 26 , wherein said plant or seed is corn, soybean, cotton, canola, alfalfa, wheat, rice, sugarcane, or sugar beet.