US2018193252A1PendingUtilityA1

Cosmetic Compositions and Method of Making the Same

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Assignee: SERUCELL CORPPriority: Jan 15, 2014Filed: Mar 5, 2018Published: Jul 12, 2018
Est. expiryJan 15, 2034(~7.5 yrs left)· nominal 20-yr term from priority
Inventors:Walter De Paula Neto
A61Q 17/04A61Q 19/00A61K 8/676A61Q 19/007A61K 2800/70A61K 8/985A61K 8/981A61K 8/671A61Q 19/08
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Claims

Abstract

The present invention generally relates to cosmetic compositions comprising serum derived from cultured keratinocyte and fibroblast cells. The invention also relates to methods of producing serum for use in the cosmetic compositions through novel cell culture techniques.

Claims

exact text as granted — not AI-modified
1 . A cosmetic composition comprising:
 a keratinocyte fibroblast serum (KFS), the KFS being a combination of compounds secreted by keratinocytes and fibroblast in-culture in sterile media without growth factor, the combination of compounds comprising:
 tenascin; and 
 a protease formulation. 
   
     
     
         2 - 20  (canceled) 
     
     
         21 . The composition of  claim 1 , wherein the protease formulation comprises serine protease. 
     
     
         22 . The composition of  claim 1 , wherein the protease formulation comprises aminopeptidase N. 
     
     
         23 . The composition of  claim 1 , wherein the KFS further comprises an extracellular matrix formulation. 
     
     
         24 . The composition of  claim 23 , wherein the extracellular matrix formulation comprises laminin. 
     
     
         25 . The composition of  claim 24 , wherein the laminin comprises laminin subunit beta-3. 
     
     
         26 . The composition of  claim 24 , wherein the laminin comprises laminin subunit gamma-1. 
     
     
         27 . The composition of  claim 24 , wherein the laminin comprises laminin subunit alpha-4. 
     
     
         28 . The composition of  claim 24 , wherein the laminin comprises laminin subunit beta-1. 
     
     
         29 . The composition of  claim 24 , wherein the laminin comprises laminin-431. 
     
     
         30 . The composition of  claim 23 , wherein the extracellular matrix formulation comprises fibronectin isoform 3. 
     
     
         31 . The composition of  claim 23 , wherein the extracellular matrix formulation comprises transforming growth factor-beta-induced protein ig-h3. 
     
     
         32 . The composition of  claim 1 , further comprising an emulsifier. 
     
     
         33 . The composition of  claim 31 , wherein the emulsifier comprises a fatty alcohol. 
     
     
         34 . The composition of  claim 1 , further comprising an emollient. 
     
     
         35 . The composition of  claim 1 , further comprising a humectant. 
     
     
         36 . The composition of  claim 1 , further comprising an antioxidant. 
     
     
         37 . The composition of  claim 1 , further comprising a preservative. 
     
     
         38 . The composition of  claim 1 , further comprising lipids. 
     
     
         39 . The composition of  claim 1 , further comprising:
 water;   vitamin A; and   vitamin C.

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