Polypeptides Having Peroxygenase Activity

Abstract

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

1 .- 43 . (canceled) 
     
     
         44 . A nucleic acid construct or expression vector comprising a polynucleotide encoding polypeptide having peroxygenase activity, wherein the polypeptide has at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 2 and the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in an expression host. 
     
     
         45 . The nucleic acid construct or expression vector of  claim 44 , wherein the polypeptide has at least 85% sequence identity to the mature polypeptide of SEQ ID NO: 2. 
     
     
         46 . The nucleic acid construct or expression vector of  claim 44 , wherein the polypeptide has at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2. 
     
     
         47 . The nucleic acid construct or expression vector of  claim 44 , wherein the polypeptide has at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2. 
     
     
         48 . The nucleic acid construct or expression vector of  claim 44 , wherein the polypeptide is encoded by a polynucleotide that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii). 
     
     
         49 . The nucleic acid construct or expression vector of  claim 44 , wherein the polypeptide is encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1, or the cDNA sequence thereof. 
     
     
         50 . The nucleic acid construct or expression vector of  claim 44 , wherein the polypeptide is a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions. 
     
     
         51 . The nucleic acid construct or expression vector of  claim 44 , wherein the polypeptide is a fragment of the mature polypeptide of SEQ ID NO: 2 that has peroxygenase activity. 
     
     
         52 . The nucleic acid construct or expression vector of  claim 44 , comprising or consisting of SEQ ID NO: 2, or the mature polypeptide of SEQ ID NO: 2. 
     
     
         53 . The nucleic acid construct or expression vector of  claim 52 , wherein the mature polypeptide is amino acids 1 to 241 of SEQ ID NO: 2. 
     
     
         54 . The nucleic acid construct or expression vector of  claim 44 , which comprises an amino acid sequence including the motif: 
       
         
           
                 
                 
               
                     
                   E-H-D-[G,A]-S-[L,I]-S-R, 
                 
                     
                   or 
                 
                     
                     
                 
                     
                   R-[G,A]-P-C-P-X-[M,L]-N-[S,T]-L. 
                 
             
                
                
                
                
               
            
           
         
       
     
     
         55 . A recombinant host cell comprising the nucleic acid construct of  claim 44 .