Polypeptides Having Endoglucanase Activity And Polynucleotides Encoding Same

Abstract

The present invention relates to isolated polypeptides having endoglucanase activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.

1 - 20 . (canceled) 
     
     
         21 . A method for degrading a cellulosic material, comprising: treating the cellulosic material with an enzyme composition comprising a polypeptide having endoglucanase activity, wherein the polypeptide having endoglucanase activity is selected from the group consisting of:
 (a) a polypeptide having endoglucanase activity having at least 95% sequence identity to amino acids 18 to 419 of SEQ ID NO: 2; and   (b) a polypeptide having endoglucanase activity encoded by a polynucleotide having at least 95% sequence identity to nucleotides 51 to 1257 of SEQ ID NO: 1;   (c) a polypeptide comprising a catalytic domain having endoglucanase activity having at least 95% sequence identity to amino acids 93 to 419 of SEQ ID NO: 2; and   (d) a polypeptide comprising a catalytic domain having endoglucanase activity encoded by a polynucleotide having at least 95% sequence identity to nucleotides 277 to 1317 of SEQ ID NO: 1.   
     
     
         22 . The method of  claim 21 , wherein the polypeptide having endoglucanase activity has at least 96% sequence identity to amino acids 18 to 419 of SEQ ID NO: 2. 
     
     
         23 . The method of  claim 21 , wherein the polypeptide having endoglucanase activity has at least 97% sequence identity to amino acids 18 to 419 of SEQ ID NO: 2. 
     
     
         24 . The method of  claim 21 , wherein the polypeptide having endoglucanase activity has at least 98% sequence identity to amino acids 18 to 419 of SEQ ID NO: 2. 
     
     
         25 . The method of  claim 21 , wherein the polypeptide having endoglucanase activity has at least 99% sequence identity to amino acids 18 to 419 of SEQ ID NO: 2. 
     
     
         26 . The method of  claim 21 , wherein the polypeptide having endoglucanase activity comprises amino acids 18 to 419 of SEQ ID NO: 2. 
     
     
         27 . The method of  claim 21 , wherein the catalytic domain has at least 96% sequence identity to amino acids 93 to 419 of SEQ ID NO: 2. 
     
     
         28 . The method of  claim 21 , wherein the catalytic domain has at least 97% sequence identity to amino acids 93 to 419 of SEQ ID NO: 2. 
     
     
         29 . The method of  claim 21 , wherein the catalytic domain has at least 98% sequence identity to amino acids 93 to 419 of SEQ ID NO: 2. 
     
     
         30 . The method of  claim 21 , wherein the catalytic domain has at least 99% sequence identity to amino acids 93 to 419 of SEQ ID NO: 2. 
     
     
         31 . The method of  claim 21 , wherein the catalytic domain comprises amino acids 93 to 419 of SEQ ID NO: 2. 
     
     
         32 . The method of  claim 21 , wherein the cellulosic material is pretreated. 
     
     
         33 . The method of  claim 21 , wherein the enzyme composition further comprises one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin. 
     
     
         34 . The method of  claim 33 , wherein the cellulase is one or more enzymes selected from the group consisting of an endoglucanase, a cellobiohydrolase, and a beta-glucosidase. 
     
     
         35 . The method of  claim 33 , wherein the hemicellulase is one or more enzymes selected from the group consisting of a xylanase, an acetylxylan esterase, a feruloyl esterase, an arabinofuranosidase, a xylosidase, and a glucuronidase. 
     
     
         36 . The method of  claim 21 , further comprising recovering the degraded cellulosic material. 
     
     
         37 . The method of  claim 36 , wherein the degraded cellulosic material is a sugar. 
     
     
         38 . The method of  claim 37 , wherein the sugar is selected from the group consisting of glucose, xylose, mannose, galactose, and arabinose.