Novel assay to detect human periostin

Abstract

This disclosure provides a robust, sensitive, and specific assay for the detection and measurement of periostin levels in samples obtained from human patients having, or suspected of having an IL-13-mediated disease or disorder. The disclosure further provides novel antiperiostin monoclonal antibodies that recognize at least isoforms 1, 2, 3, 4, 7, and 8 of human periostin, and assay kits comprising one or more of these antibodies.

1 - 18 . (canceled) 
     
     
         19 . An isolated nucleic acid molecule encoding an isolated antibody or antigen-binding fragment thereof which binds to the same periostin epitope as monoclonal antibody 4B4.B11 produced from a hybridoma deposited at the American Type Culture Collection (ATCC) under Deposit No. PTA-120210. 
     
     
         20 . A vector comprising the isolated nucleic acid molecule of  claim 19 . 
     
     
         21 . A host cell comprising the vector of  claim 20 . 
     
     
         22 . (canceled) 
     
     
         23 . The host cell of  claim 21 , which is an  E. coli  cell. 
     
     
         24 . (canceled) 
     
     
         25 . The host cell of  claim 21 , which is a eukaryotic cell selected from protist cells, animal cells, plants cells, and fungal cells. 
     
     
         26 . The host cell of  claim 25 , which is an animal cell selected from a mammalian cell, an avian cell, and an insect cell. 
     
     
         27 . The host cell of  claim 25 , which is a CHO cell, a COS cell, a NSO cell, or a yeast cell. 
     
     
         28 . A kit comprising the isolated nucleic acid molecule of  claim 19  and instructions for use. 
     
     
         29 . A kit comprising the vector of  claim 20  and instructions for use. 
     
     
         30 . A kit comprising the host cell of  claim 21  and instructions for use. 
     
     
         31 . A kit comprising the host cell of  claim 27  and instructions for use. 
     
     
         32 . An isolated nucleic acid molecule encoding an isolated antibody or antigen-binding fragment thereof which binds to the same periostin epitope as monoclonal antibody 7B5.C4 produced from a hybridoma deposited at the ATCC under Deposit No. PTA-120211. 
     
     
         33 . A vector comprising the isolated nucleic acid molecule of  claim 32 . 
     
     
         34 . A host cell comprising the vector of  claim 33 . 
     
     
         35 . The host cell of  claim 34 , which is an  E. coli  cell. 
     
     
         36 . The host cell of  claim 34 , which is a eukaryotic cell selected from protist cells, animal cells, plants cells, and fungal cells. 
     
     
         37 . The host cell of  claim 36 , which is an animal cell selected from a mammalian cell, an avian cell, and an insect cell. 
     
     
         38 . The host cell of  claim 36 , which is a CHO cell, a COS cell, a NSO cell, or a yeast cell. 
     
     
         39 . A kit comprising the isolated nucleic acid molecule of  claim 32  and instructions for use. 
     
     
         40 . A kit comprising the vector of  claim 33  and instructions for use. 
     
     
         41 . A kit comprising the host cell of  claim 34  and instructions for use. 
     
     
         42 . A kit comprising the host cell of  claim 38  and instructions for use. 
     
     
         43 . An isolated nucleic acid molecule encoding an isolated antibody or antigen-binding fragment thereof which binds to the same periostin epitope as monoclonal antibody 3C11.G5 produced from a hybridoma deposited at the ATCC under Deposit No. PTA-120209. 
     
     
         44 . A vector comprising the isolated nucleic acid molecule of  claim 43 . 
     
     
         45 . A host cell comprising the vector of  claim 44 . 
     
     
         46 . The host cell of  claim 45 , which is an  E. coli  cell. 
     
     
         47 . The host cell of  claim 45 , which is a eukaryotic cell selected from protist cells, animal cells, plants cells, and fungal cells. 
     
     
         48 . The host cell of  claim 47 , which is an animal cell selected from a mammalian cell, an avian cell, and an insect cell. 
     
     
         49 . The host cell of  claim 47 , which is a CHO cell, a COS cell, a NSO cell, or a yeast cell. 
     
     
         50 . A kit comprising the isolated nucleic acid molecule of  claim 43  and instructions for use. 
     
     
         51 . A kit comprising the vector of  claim 44  and instructions for use. 
     
     
         52 . A kit comprising the host cell of  claim 45  and instructions for use. 
     
     
         53 . A kit comprising the host cell of  claim 49  and instructions for use.