Nutritional formulations including human milk oligosaccharides and antioxidants and uses thereof

Abstract

Disclosed are nutritional compositions including human milk oligosaccharides in combination with long chain polyunsaturated fatty acids and/or carotenoids that can be administered to preterm infants, term infants, toddlers, and children for reducing inflammation and the incidence of inflammatory diseases.

1 . A nutritional powder comprising a human milk oligosaccharide selected from the group consisting of 6′-sialyllactose, 3′-sialyllactose, 2′-fucosyllactose, 3-fucosyllactose, lacto-N-tetraose, lacto-N-neotetraose, lacto-N-fucopentaose, and combinations thereof and docosahexaenoic acid in an amount of about 3 mg per 100 kcals to about 16 mg per 100 kcals. 
     
     
         2 . The nutritional powder of  claim 1 , wherein the nutritional powder further comprises arachidonic acid in an amount of about 10 mg per 100 kcals to about 31 mg per 100 kcals. 
     
     
         3 . The nutritional powder of  claim 1 , wherein the human milk oligosaccharide is present in an amount of from about 0.01 mg/mL to about 20 mg/mL and is selected from the group consisting of 6′-sialyllactose, 3′-sialyllactose, 2′-fucosyllactose, lacto-N-neotetraose, and combinations thereof. 
     
     
         4 . The nutritional powder of  claim 1 , wherein the nutritional powder further comprises a long chain polyunsaturated fatty acid selected from the group consisting of linoleic acid, linolenic acid, arachidonic acid, eicosapentaenoic acid, docosapentaenoic acid, and combinations thereof. 
     
     
         5 . The nutritional powder of  claim 1 , wherein the human milk oligosaccharide is derived from a fermentation process, an enzymatic process, a chemical process, or a combination thereof. 
     
     
         6 . The nutritional powder of  claim 1 , wherein the human milk oligosaccharide is 6′-sialyllactose. 
     
     
         7 . The nutritional powder of  claim 1 , wherein the human milk oligosaccharide is 2′-fucosyllactose. 
     
     
         8 . The nutritional powder of  claim 7 , wherein the 2′-fucosyllactose is present in a concentration of about 0.01 mg/mL to less than 2 mg/mL. 
     
     
         9 . The nutritional powder of  claim 1 , further comprising about 0.001 μg/mL to about 5 μg/mL of a carotenoid. 
     
     
         10 . (canceled) 
     
     
         11 . The nutritional powder of  claim 9 , wherein the carotenoid is selected from the group consisting of lutein, lycopene, beta-carotene, and combinations thereof. 
     
     
         12 . The nutritional powder of  claim 1  further comprising a nucleotide in an amount of from about 10 mg/L to about 200 mg/L. 
     
     
         13 . The nutritional powder of  claim 12 , wherein the nucleotide is present in an amount of from about 42 mg/L to about 102 mg/L. 
     
     
         14 . A nutritional powder comprising a human milk oligosaccharide selected from the group consisting of 6′-sialyllactose, 3′-sialyllactose, 2′-fucosyllactose, 3-fucosyllactose, lacto-N-tetraose, lacto-N-neotetraose, lacto-N-fucopentaose, and combinations thereof, docosahexaenoic acid in an amount of about 3 mg per 100 kcals to about 16 mg per 100 kcals, and a carotenoid in an amount of about 0.001 μg/mL to about 5 μg/mL. 
     
     
         15 . The nutritional powder of  claim 14 , wherein the human milk oligosaccharide is selected from the group consisting of 6′-sialyllactose, 3′-sialyllactose, 2′-fucosyllactose, lacto-N-neotetraose, and combinations thereof. 
     
     
         16 . The nutritional powder of  claim 14 , wherein the human milk oligosaccharide is derived from a source selected from human, bovine, ovine, porcine, caprine, or combinations thereof. 
     
     
         17 . The nutritional powder of  claim 14  further comprising a nucleotide in an amount of about 10 mg/L to about 200 mg/L. 
     
     
         18 . The nutritional powder of  claim 14 , wherein the nutritional powder further comprises arachidonic acid in an amount of about 10 mg per 100 kcals to about 31 mg per 100 kcals. 
     
     
         19 . (canceled) 
     
     
         20 . The nutritional powder of  claim 14 , wherein the carotenoid is selected from the group consisting of lutein, lycopene, beta-carotene, and combinations thereof. 
     
     
         21 . The nutritional powder of  claim 14 , wherein the human milk oligosaccharide is present in an amount of about 0.01 mg/mL to about 20 mg/mL. 
     
     
         22 . A method of modulating immunity in an infant, toddler, or child in need thereof, the method comprising administering to the infant, child or toddler a nutritional powder comprising a human milk oligosaccharide, docosahexaenoic acid in an amount of from about 3 mg per 100 kcals to about 16 mg per 100 kcals, and from about 0.001 μg/mL to about 5 μg/mL of a carotenoid. 
     
     
         23 . The method of  claim 22 , wherein the nutritional powder further comprises arachidonic acid in an amount of about 10 mg per 100 kcals to about 31 mg per 100 kcals. 
     
     
         24 . The method of  claim 22 , wherein the human milk oligosaccharide is selected from the group consisting of 6′-sialyllactose, 3′-sialyllactose, 2′-fucosyllactose, 3-fucosyllactose, lacto-N-tetraose, lacto-N-neotetraose, lacto-N-fucopentaose, and combinations thereof. 
     
     
         25 . The method of  claim 22 , wherein the human milk oligosaccharide is selected from the group consisting of 6′-sialyllactose, 3′-sialyllactose, 2′-fucosyllactose, lacto-N-neotetraose, and combinations thereof. 
     
     
         26 . (canceled) 
     
     
         27 . The method of  claim 22 , wherein the carotenoid is selected from the group consisting of lutein, lycopene, beta-carotene, and combinations thereof. 
     
     
         28 . The method of  claim 22 , wherein the nutritional powder further comprises a nucleotide in an amount of about 10 mg/L to about 200 mg/L. 
     
     
         29 . A nutritional powder comprising a human milk oligosaccharide selected from the group consisting of 6′-sialyllactose, 3′-sialyllactose, 2′-fucosyllactose, 3-fucosyllactose, lacto-N-tetraose, lacto-N-neotetraose, lacto-N-fucopentaose, and combinations thereof, docosahexaenoic acid in an amount of about 3 mg per 100 kcals to about 16 mg per 100 kcals and arachidonic acid in an amount of about 10 mg per 100 kcals to about 31 mg per 100 kcals. 
     
     
         30 . The nutritional powder of  claim 29  further comprising a nucleotide in an amount of about 10 mg/L to about 200 mg/L.