Bioprobes and methods of use thereof

Abstract

Disclosed are biomolecule based bioprobes that exhibit improved water solubility and mono layer-forming properties with substantially little or no aggregation that can appreciably interfere with binding of the bioprobes to a target nucleotide. The bioprobes may be used in conjunction with a suitable reporter system to detect very small quantities of biological markers. The bio-probes comprise a nucleobase sequence capable of hybridizing to a target nucleotide; and at least one charged functional group attached to said nucleobase sequence. Also disclosed are biosensors, and sensing devices that comprise the bin-probe. Further disclosed are suitable electrochemical reporter systems for use with the bioprobes. Methods of use of these devices and probes, including for the detection of target biomarkers, including biomarkers for cancer cells or pathogens, are also included.

1 . A bio-probe comprising:
 a nucleobase sequence capable of hybridizing to a target nucleotide; at least one charged functional group attached to said nucleobase sequence, wherein said charged functional group comprises a cationic functional group, an anionic functional group, a charged amino acid, or a combination thereof; and   wherein attachment of said charged functional group to said nucleobase results in lesser aggregation of a plurality of bio-probes, as compared to bio-probes not comprising a charged functional group attached to said nucleobase.   
     
     
         2 - 116 . (canceled)