Methods for diagnosing diseases associated with human signal peptide-containing molecules

Abstract

The invention provides human signal peptide-containing proteins (HSPP) and polynucleotides which identify and encode HSPP. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating, or preventing disorders associated with expression of HSPP.

We claim: 
     
         1 . An isolated polynucleotide encoding a polypeptide comprising an amino acid sequence having at least about 95% sequence identity to an amino acid sequence of SEQ ID NO: 43, wherein the homologous polypeptide has a silent change in its amino acid sequence relative to the polypeptide represented by SEQ ID NO: 43. 
     
     
         2 . The isolated polynucleotide of  claim 1 , wherein the polynucleotide encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 43. 
     
     
         3 . The isolated polynucleotide of  claim 1 , comprising a polynucleotide sequence of SEQ ID NO: 177. 
     
     
         4 . A recombinant polynucleotide comprising a promoter sequence operably linked to the polynucleotide of  claim 1 . 
     
     
         5 . An isolated cell transformed with the recombinant polynucleotide of  claim 4 . 
     
     
         6 . A method of producing a polypeptide comprising:
 a) culturing a cell under conditions suitable for expression of the polypeptide, wherein the cell is transformed with a recombinant polynucleotide, and the recombinant polynucleotide comprises a promoter sequence operably linked to the polynucleotide of  claim 1 , and   b) recovering the polypeptide so expressed.   
     
     
         7 . The method of  claim 6 , wherein the recombinant polynucleotide comprises the polynucleotide sequence of SEQ ID NO: 177. 
     
     
         8 . An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of:
 a) a polynucleotide sequence having at least about 95% sequence identity to a polynucleotide sequence of SEQ ID NO: 177, wherein the homologous polynucleotide encodes a polypeptide with at least one functional characteristic of the polypeptide encoded by the polynucleotide sequence of SEQ ID NO: 177;   b) a polynucleotide sequence complementary to the polynucleotide sequence of a); and   c) an RNA equivalent of the polynucleotide sequence of a) or b).   
     
     
         9 . The isolated polynucleotide of  claim 8 , comprising the polynucleotide sequence of SEQ ID NO: 177. 
     
     
         10 . An isolated polypeptide selected from the group consisting of:
 a) a polypeptide comprising the amino acid sequence of SEQ ID NO: 43;   b) a polypeptide comprising a naturally occurring amino acid sequence at least 90% identical to the amino acid sequence of SEQ ID NO: 43;   c) a biologically active fragment of a polypeptide having the amino acid sequence of SEQ ID NO: 43; and   d) an immunogenic fragment of a polypeptide having the amino acid sequence of SEQ ID NO: 43.   
     
     
         11 . The isolated polypeptide of  claim 10 , comprising a naturally occurring amino acid sequence at least 95% identical to the amino acid sequence of SEQ ID NO: 43. 
     
     
         12 . The isolated polypeptide of  claim 10 , comprising an amino acid sequence of SEQ ID NO: 43.