Method of measuring hematocrit and method of testing blood

Abstract

A method of measuring hematocrit and a method of testing blood are provided. The method for measuring hematocrit includes the following steps. A test strip is provided. The test strip includes a reaction region and a pair of electrodes disposed in the reaction region. A whole blood sample is entered to the reaction region. After the whole blood sample enters the reaction region, a set of square wave voltages is applied to the pair of electrodes based on a square wave voltammetry method to obtain a feedback related to hematocrit. A difference between an initial time when the whole blood sample enters the reaction region and an initial time when the set of square wave voltage is applied ranges from 0.1 seconds to 200 seconds. A hematocrit value is calculated according to the feedback.

What is claimed is: 
     
         1 . A method of measuring hematocrit, comprising:
 providing a test strip comprising a reaction region and a pair of electrodes disposed in the reaction region;   entering a whole blood sample into the reaction region;   applying a set of square wave voltages to the pair of electrodes based on a square wave voltammetry method after the whole blood sample enters the reaction region to obtain a feedback related to hematocrit, wherein an interval between an initial time when the whole blood sample enters the reaction region and an initial time when the set of square wave voltages is applied ranges from 0.1 seconds to 200 seconds; and   calculating a hematocrit value based on the feedback.   
     
     
         2 . The method of measuring hematocrit as claimed in  claim 1 , wherein the pair of electrodes comprise a working electrode and a reference electrode, a distance between the working electrode and the reference electrode ranges from 0.01 mm to 5 mm, and/or a ratio of an area of the working electrode to an area the reference electrode range from 1 to 1.5. 
     
     
         3 . The method of measuring hematocrit as claimed in  claim 1 , wherein a frequency of the set of square wave voltages ranges from 100 Hz to 4000 Hz, an amplitude of the set of square wave voltages is greater than or equal to 0.01 V, and/or a voltage increment in the set of square wave voltages ranges from 0.01 V to 0.4 V. 
     
     
         4 . The method of measuring hematocrit as claimed in  claim 1 , wherein a voltage sweep range of the set of square wave voltages ranges from 0 V to 0.8 V, and/or a duration of application of the set of square wave voltages ranges from 0.01 seconds to 4 seconds. 
     
     
         5 . A method of testing blood, comprising:
 providing a test strip comprising a first reaction region, a pair of first electrodes disposed in the first reaction region, a second reaction region, and a pair of second electrodes disposed in the second reaction region;   entering a whole blood sample into the first reaction region and the second reaction region;   applying two sets of square wave voltages to the pair of first electrodes based on a square wave voltammetry method after the whole blood sample enters the first reaction region, so as to respectively obtain a first feedback and a second feedback related to hematocrit;   applying a voltage to the pair of second electrodes to obtain a third feedback and thereby calculate a concentration of a target analyte in the whole blood sample;   determining whether a ratio of the first feedback with respect to the second feedback falls within a predetermined range;   adopting the calculated concentration of the target analyte if the ratio of the first feedback with respect to the second feedback falls within the predetermined range, and providing a message of anomalous testing if the ratio of the first feedback with respect to the second feedback does not fall within the predetermined range.   
     
     
         6 . The method of testing blood as claimed in  claim 5 , wherein an interval between an initial time when the whole blood sample enters the reaction region and an initial time of the two sets of square wave voltages ranges from 0.1 seconds to 200 seconds. 
     
     
         7 . The method of testing blood as claimed in  claim 5 , wherein the predetermined range ranges from 0.85 to 1.18. 
     
     
         8 . The method of testing blood as claimed in  claim 5 , wherein calculating the concentration of the target analyte in the whole blood sample based on the third feedback comprises calculating the concentration of the target analyte based on a hematocrit value based on at least one of the first feedback and the second feedback and the third feedback. 
     
     
         9 . The method of testing blood as claimed in  claim 5 , wherein an interval between the two sets of square wave voltages ranges from 0.1 seconds to 200 seconds. 
     
     
         10 . The method of testing blood as claimed in  claim 5 , wherein the target analyte comprises blood glucose, glycated hemoglobin (HbA1c), blood lactic acid, cholesterol, uric acid, triglyceride, coagulation factor, or anticoagulant. 
     
     
         11 . The method of testing blood as claimed in  claim 5 , wherein a reactive reagent is provided on the first reaction region and the second reaction region. 
     
     
         12 . The method of testing blood as claimed in  claim 5 , wherein a reactive reagent is provided on the second reaction region. 
     
     
         13 . A method of measuring hematocrit, comprising:
 providing a test strip comprising a reaction region and a pair of electrodes disposed in the reaction region;   entering a whole blood sample into the reaction region;   continuously applying a plurality of sets of square wave voltages to the pair of electrodes based on a square wave voltammetry method to obtain a plurality of feedbacks related to hematocrit, wherein an interval between two adjacent sets of square wave voltages ranges from 0.1 seconds to 4 seconds;   obtaining a feedback of an n-th set of square wave voltages to calculate a hematocrit value of the whole blood sample, wherein n is a positive integer greater than 1.   
     
     
         14 . A method of testing blood, comprising:
 providing a test strip comprising a first reaction region, a pair of first electrodes disposed in the first reaction region, a second reaction region, and a pair of second electrodes disposed in the second reaction region;   entering a whole blood sample into the first reaction region and the second reaction region;   continuously applying a plurality of sets of square wave voltages to the pair of first electrodes based on a square wave voltammetry method to obtain a plurality of feedbacks related to hematocrit;   applying a voltage to the pair of second electrodes to calculate a concentration of a target analyte in the whole blood sample;   determining whether a ratio of two arbitrary feedbacks of the square wave voltages after the first set falls within a predetermined range;   adopting the calculated concentration of the target analyte if the ratio of the two arbitrary feedbacks of the square wave voltages after the first set falls within the predetermined range, and providing a message of anomalous testing if the ratio of the two arbitrary feedbacks of the square wave voltages after the first set does not fall within the predetermined range.   
     
     
         15 . The method of testing blood as claimed in  claim 14 , wherein the predetermined range ranges from 0.85 to 1.18. 
     
     
         16 . The method of testing blood as claimed in  claim 14 , wherein calculating the concentration of the target analyte in the whole blood sample comprises calculating the concentration of the target analyte based on a hematocrit value based on a feedback of an arbitrary set of square wave voltages after the first set or an average feedback of a plurality of sets of square wave voltages after the first set and a feedback obtained by applying the voltage to the pair of second electrodes. 
     
     
         17 . The method of testing blood as claimed in  claim 14 , wherein an interval between two adjacent sets of square wave voltages ranges from 0.1 seconds to 4 seconds.