Method for measuring blood components and biosensor and measuring instrument for use therein

Abstract

The present invention provides a method of measuring a component in blood, by which the amounts of blood cells and an interfering substance can be measured with high accuracy and high reliability and the amount of the component can be corrected accurately based on the amounts of the blood cells and the interfering substance. In a sensor for measuring a blood component, a first working electrode 13 measures a current that flows during a redox reaction of a blood component, a second working electrode 17 measures the amount of blood cells, and a third working electrode 12 measures the amount of an interfering substance. Next, based on the measurement results, the amount of the blood component to be measured is corrected. Thus, more accurate and precise measurement of the amount of the blood component can be realized.

1 - 25 . (canceled) 
     
     
         26 . A method of measuring a glucose in blood by using a biosensor,
 the biosensor comprising:   an electrode system comprising an electrode on which a reagent is disposed and an electrode on which no reagent is disposed, said reagent including an oxidoreductase that acts upon the glucose and a mediator,   
       the method comprising:
 a first step of obtaining a first current value by applying a first voltage to the electrode system, said first current value including an effect of an interfering substance and an effect of blood cells or hematocrit (Hct), 
 a second step of obtaining a second current value by applying a second voltage to the electrode system, said second current value determining the effect of blood cells or hematocrit (Hct), 
 
       and thereafter,
 a third step of obtaining a corrected glucose value by using the first current value and the second current value. 
 
     
     
         27 . The method of measuring a glucose in blood by using a biosensor according to  claim 26 , wherein the first voltage is applied for a time of 0.01 to 5 seconds. 
     
     
         28 . The method of measuring a glucose in blood by using a biosensor according to  claim 26 , wherein the second voltage is applied for a time of 0.01 to 5 seconds. 
     
     
         29 . The method of measuring a glucose in blood by using a biosensor according to  claim 26 , wherein the first step is performed before the second step.