Analyte Detection

Abstract

The present disclosure provides methods and/or kits for detecting an analyte in a sample. Some embodiments provide a method for detecting a non-nucleic acid analyte in a sample using a solid substrate comprising a bound immobilisation agent and an antibody capture agent and a detectable agent, which can bind to the analyte. The antibody capture agent comprises, at a plurality of sites, a ligand for the immobilisation agent. A complex between the analyte, the antibody capture agent and a detectable agent is formed and immobilised on the solid substrate by binding between the immobilisation agent and the ligand. In some embodiments, the ligand and the immobilisation agent are a binding pair comprising a peptide tag and an anti-peptide tag antibody.

1 - 25 . (canceled) 
     
     
         26 . A method for preparing a series of assay kits, comprising:
 i) preparing a series of substrates comprising a quantity of antibodies, the antibodies configured to selectively bind with the a certain type of peptide;   ii) further preparing a series of solutions comprising a quantity of antibody capture agents covalently conjugated to at least one of the certain type of peptide; and   iii) assembling the series of assay kits, the series of assay kits comprising:
 a) at least one substrate from the series of substrates, and 
 b) at least one solution from the series of solutions. 
   
     
     
         27 . The method of  claim 26 , wherein the series of assay kits comprise:
 a) a first assay kit comprising a first substrate of the series of substrates and a first solution of the series of solutions, the first solution comprising a quantity of first antibody capture agents; and   b) a second assay kit comprising a second substrate of the series of substrates and second solution of the series of solutions, the second solution comprising   a quantity of second antibody capture agents.   
     
     
         28 . The method of  claim 27 , wherein the quantity of first antibody capture agents comprises a first type of antibody and the quantity of second antibody capture agents comprises a second type of antibody, the first type of antibody different from the second type of antibody. 
     
     
         29 . The method of  claim 27 , wherein each first antibody capture agent present in at least a portion of the quantity of first antibody capture agents is covalently conjugated to a plurality of the certain type of peptide. 
     
     
         30 . The method of  claim 26 , wherein the quantity of antibodies is obtained from a single batch of antibodies. 
     
     
         31 . The method of  claim 26 , wherein the certain type of peptide comprises the amino acid sequence DYKDDDDK (SEQ ID NO: 1). 
     
     
         32 . The method of  claim 26 , wherein at least one of the series of substrates is an assay plate. 
     
     
         33 . The method of  claim 29 , wherein the assay plate is a multi-well plate. 
     
     
         34 . The method of  claim 26 , wherein at least one of the series of substrates is a microfluidic device. 
     
     
         35 . The method of  claim 26 , wherein at least one of the series of substrates is a multi-well strip. 
     
     
         36 . The method of  claim 26 , wherein at least one of the series of substrates is a bead. 
     
     
         37 . The method of  claim 26 , wherein at least one of the series of substrates is a dip stick. 
     
     
         38 . A series of analyte detection kits, comprising:
 i) a series of substrates comprising a quantity of anti-peptide antibodies, the anti-peptide antibodies configured to selectively bind with the a certain type of peptide; and   ii) a series of solutions comprising quantities of at least two different types of antibody capture agents, each of the at least two different types of antibody capture agents covalently conjugated a plurality of the certain type of peptide.   
     
     
         39 . The series of analyte detection kits of  claim 38 , wherein at least one of the series of substrates is a multi-well plate comprising a plurality of reaction vessels coated therein with at least a portion of the quantity of anti-peptide antibodies. 
     
     
         40 . The series of analyte detection kits of  claim 38 , wherein at least a portion of the quantity of anti-peptide antibodies is coated at a concentration of 25 μg/ml or less. 
     
     
         41 . The series of analyte detection kits of  claim 38 , wherein the series of analyte detection kits are configured to detect analytes in a time of 2 hours or less. 
     
     
         42 . The series of analyte detection kits of  claim 38 , wherein the series of analyte detection kits is configured to detect analytes after no more than one washing. 
     
     
         43 . The series of analyte detection kits of  claim 38 , comprising a further series of solutions, the further series of solutions comprising detectable agents. 
     
     
         44 . The plural analyte detection kits of  claim 43 , wherein the detectable agents comprise detectable tags. 
     
     
         45 . A method for preparing a series of assay kits, comprising:
 i) procuring a series of substrates comprising a quantity of antibodies, the antibodies configured to selectively bind with the a certain type of peptide;   ii) further procuring a series of solutions comprising a quantity of antibody capture agents covalently conjugated to at least one of the certain type of peptide; and   iii) assembling the series of assay kits, each one of the series of assay kits comprising:
 a) at least one substrate from the series of substrates, and 
 b) at least one solution from the series of solutions.