Multipotent Adult Stem Cells and Methods for Isolation

Abstract

The invention provides isolated stem cells of non-embryonic origin that can be maintained in culture in the undifferentiated state or differentiated to form cells of multiple tissue types. Also provided are methods of isolation and culture, as well as therapeutic uses for the isolated cells.

1 - 63 . (canceled) 
     
     
         64 . A method for treating parietal infarction in a subject, the method comprising administering isolated expanded human non-embryonic non-germ cells, the cells having undergone at least 10-40 cell doublings in culture, wherein the cells express telomerase and have a normal karyotype, to the subject in a therapeutically effective amount and for a sufficient time to treat parietal infarction in the subject. 
     
     
         65 . The method of  claim 64  wherein stroke is treated in the subject. 
     
     
         66 . The method of  claim 64  wherein the non-embryonic, non-germ cells further express oct4. 
     
     
         67 . The method of  claim 64  wherein the non-embryonic, non-germ cells can differentiate into at least one cell type of at least two of the endodermal, ectodermal, and mesodermal embryonic lineages. 
     
     
         68 . The method of  claim 67  wherein the non-embryonic, non-germ cells can differentiate into at least one cell type of each of the endodermal, ectodermal, and mesodermal embryonic lineages. 
     
     
         69 . The method of  claim 66  wherein the non-embryonic, non-germ cells can differentiate into at least one cell type of at least two of the endodermal, ectodermal, and mesodermal embryonic lineages. 
     
     
         70 . The method of  claim 69  wherein the non-embryonic, non-germ cells can differentiate into at least one cell type of each of the endodermal, ectodermal, and mesodermal embryonic lineages. 
     
     
         71 . The method of  claim 64  wherein the non-embryonic, non-germ cells have undergone greater than 40 cell doublings. 
     
     
         72 . The method of  claim 66  wherein the non-embryonic, non-germ cells have undergone greater than 40 cell doublings. 
     
     
         73 . The method of  claim 67  wherein the non-embryonic, non-germ cells have undergone greater than 40 cell doublings. 
     
     
         74 . The method of  claim 68  wherein the non-embryonic, non-germ cells have undergone greater than 40 cell doublings. 
     
     
         75 . The method of  claim 69  wherein the non-embryonic, non-germ cells have undergone greater than 40 cell doublings. 
     
     
         76 . The method of  claim 70  wherein the non-embryonic, non-germ cells have undergone greater than 40 cell doublings. 
     
     
         77 . The method of  claim 64  wherein said cells are derived from bone marrow. 
     
     
         78 . The method of  claim 64  wherein said cells are human. 
     
     
         79 . The method of  claim 64  wherein said subject is human. 
     
     
         80 . The method of  claim 78  wherein said subject is human. 
     
     
         81 . The method of  claim 64  wherein the cells are administered by localized administration. 
     
     
         82 . The method of  claim 64  wherein the cells are administered by systemic administration. 
     
     
         83 . The method of  claim 64  wherein the cells are administered in a matrix.