US2018105840A1PendingUtilityA1
Process for industrial production of chiral-1,1-difluoro-2-propanol
Assignee: PUBLIC UNIV CORPORATION TOYAMA PREFECTURAL UNIVPriority: Mar 3, 2015Filed: Mar 2, 2016Published: Apr 19, 2018
Est. expiryMar 3, 2035(~8.6 yrs left)· nominal 20-yr term from priority
C12N 1/16C07C 19/08C07C 31/10C12P 7/04C12P 41/002C07C 31/38
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Claims
Abstract
The present invention provides a process for industrial production of chiral-1,1-difluoro-2-propanol. More specifically, a microorganism having the activity to cause asymmetric reduction of 1,1-difluoroacetone or an enzyme having the same activity is allowed to act on 1,1-difluoroacetone, whereby chiral-1,1-difluoro-2-propanol can be produced with high optical purity and in good yield. The process for production of the present invention is easy for industrial implementation.
Claims
exact text as granted — not AI-modified1 . A process for production of chiral-1,1-difluoro-2-propanol represented by formula [2], wherein a microorganism having the activity to cause asymmetric reduction of 1,1-difluoroacetone or an enzyme having the same activity is allowed to act on 1,1-difluoroacetone represented by formula [1]:
[wherein the asterisk (*) represents an asymmetric atom].
2 . The process for production according to claim 1 , wherein the microorganism is at least one selected from the group consisting of Candida guilliermondii, Candida parapsilosis, Candida vini, Candida viswanathii, Cryptococcus laurentii, Cryptococcus curvatus, Debaryomyces maramus, Kluyveromyces marxianus, Ogataea polymorpha, Pichia anomala, Pichia farinosa, Pichia haplophila, Pichia minuta, Rhodotorula muculaginosa, Saccharomyces rouxii, Torulaspora delbrueckii, Wickerhamomyces subpelliculosa, and Zygosaccharomyces rouxii.
3 . The process for production according to claim 2 , wherein the microorganism is selected from the microorganisms having Accession Nos. indicated in the table below.
TABLE 1
Accession
Microorganism
No.
Depository institution
Candida guilliermondii
NBRC10279
National Institute of
Technology and Evaluation,
Independent Administrative
Agency
Candida parapsilosis
NBRC0708
National Institute of
Technology and Evaluation,
Independent Administrative
Agency
Candida vini
NBRC1247
National Institute of
Technology and Evaluation,
Independent Administrative
Agency
Candida viswanathii
NBRC10321
National Institute of
Technology and Evaluation,
Independent Administrative
Agency
Cryptococcus laurentii
NBRC0609
National Institute of
Technology and Evaluation,
Independent Administrative
Agency
Cryptococcus curvatus
NBRC1159
National Institute of
Technology and Evaluation,
Independent Administrative
Agency
Debaryomyces maramus
NBRC0668
National Institute of
Technology and Evaluation,
Independent Administrative
Agency
Kluyveromyces marxianus
NBRC10005
National Institute of
Technology and Evaluation,
Independent Administrative
Agency
Ogataea polymorpha
NBRC0799
National Institute of
Technology and Evaluation,
Independent Administrative
Agency
Pichia anomala
NBRC0118
National Institute of
Technology and Evaluation,
Independent Administrative
Agency
Pichia farinosa
NBRC0462
National Institute of
Technology and Evaluation,
Independent Administrative
Agency
Pichia haplophila
NBRC0947
National Institute of
Technology and Evaluation,
Independent Administrative
Agency
Pichia minuta
NBRC0975
National Institute of
Technology and Evaluation,
Independent Administrative
Agency
Rhodotorula muculaginosa
NBRC0001
National Institute of
Technology and Evaluation,
Independent Administrative
Agency
Saccharomyces rouxii
NBRC0493
National Institute of
Technology and Evaluation,
Independent Administrative
Agency
Torulaspora delbrueckii
NBRC0381
National Institute of
Technology and Evaluation,
Independent Administrative
Agency
Wickerhamomyces
NBRC0198
National Institute of
subpelliculosa
Technology and Evaluation,
Independent Administrative
Agency
Zygosaccharomyces rouxii
NBRC10671
National Institute of
Technology and Evaluation,
Independent Administrative
Agency
4 . The process for production according to any one of claims 1 to 3 , wherein the microorganism is allowed to act as whole microbial cells or as a cell extract thereof.
5 . The process for production according to claim 1 , wherein the enzyme is a purified enzyme derived from Ogataea polymorpha, Ogataea parapolymorpha, Pichia anomala, or Pichia minuta.
6 . The process for production according to claim 5 , wherein the Ogataea polymorpha is Ogataea polymorpha strain NBRC0799.
7 . The process for production according to claim 1 , wherein the temperature at which the microorganism or enzyme is allowed to act is 5° C. to 60° C.
8 . The process for production according to claim 1 , wherein the pH at which the microorganism or enzyme is allowed to act is in the range of 4.0 to 8.0.
9 . The process for production according to claim 1 , which further comprises a step where the mixture containing 1,1-difluoro-2-propanol and impurities obtained after the microorganism or enzyme is allowed to act is distilled to separate the impurities from the mixture and thereby purify 1,1-difluoro-2-propanol.Join the waitlist — get patent alerts
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