Anti-microbial agent from paenibacillus sp. and methods and uses thereof

Abstract

The present invention provides, in part, a Paenibacillus sp. isolate, designated Paenibacillus polymyxa JB05-01-1, as well as an anti-microbial agent obtained from the bacterium or cell culture supernatant thereof. Compositions, methods and uses are also provided.

What is claimed is: 
     
         1 . A method of inhibiting growth of a Gram-negative staining bacterium in a subject or substance in need thereof, the method comprising administering or applying an effective amount of an anti-microbial composition to the subject or substance, wherein the anti-microbial composition comprises:
 (a) an isolated  Paenibacillus polymyxa  bacterium which has a 16S ribosomal RNA (16SrRNA) gene containing a DNA comprising the base sequence of SEQ ID NO: 1;   (b) an isolated  Paenibacillus polymyxa  bacterium deposited at the ATCC® under the terms of the Budapest Treaty and designated Accession Number PTA-10436;   (c) a cell culture comprising the  Paenibacillus polymyxa  bacterium;   (d) a cell culture supernatant derived from growing the  Paenibacillus polymyxa  bacterium in a cell culture medium; or   (e) an anti-microbial agent isolated from the  Paenibacillus polymyxa  bacterium, the cell culture or the cell culture supernatant.   
     
     
         2 . The method of  claim 1 , wherein the Gram-negative staining bacterium is selected from the group consisting of  Escherichia  sp.,  Pantoea  sp.,  Pseudomonas  sp.,  Butyrivibrio  sp.,  Fibrobacter  sp.,  Salmonella  sp.,  Shigella  sp.,  Helicobacter  sp., and  Campylobacter  sp. 
     
     
         3 . The method of  claim 1 , wherein the Gram-negative staining bacterium is selected from the group consisting of  Escherichia coli  RR1,  Escherichia coli  TB1,  Escherichia coli  O157:H7,  Pantoea agglomerans  BC1,  Pseudomonas fluorescens  R73,  Butyrivibrio fibrisolvens  OR85 , Fibrobacter succinogenes  and  Pseudomonas aeruginosa.    
     
     
         4 . The method of  claim 1 , which does not inhibit the growth of a Gram-positive staining bacterium. 
     
     
         5 . The method of  claim 1 , wherein the anti-microbial agent comprises a peptide. 
     
     
         6 . The method of  claim 1 , wherein the isolated  Paenibacillus polymyxa  bacterium DNA comprises at least one gene needed for the production of a bacitracin, polymyxin, or fusaricidin. 
     
     
         7 . The method of  claim 1 , wherein the anti-microbial agent has a molecular weight between about 1000 daltons to about 2500 daltons. 
     
     
         8 . The method of  claim 1 , wherein the anti-microbial agent comprises a polymyxin. 
     
     
         9 . The method of  claim 1 , wherein the anti-microbial agent comprises at least one of colistin A and colistin B. 
     
     
         10 . The method of  claim 1 , wherein the subject is a human, a pet or an agricultural animal. 
     
     
         11 . The method of  claim 1 , wherein the anti-microbial composition is used as a probiotic. 
     
     
         12 . The method of  claim 1 , wherein the substance is selected from the group consisting of a cosmetic product, a hygiene product, a feed product, a food product and packaging material thereof. 
     
     
         13 . The method of  claim 12  wherein the food product is a dry feed product. 
     
     
         14 . A method of producing an anti-microbial agent comprising growing or culturing an isolated  Paenibacillus polymyxa  bacterium which has a 16S ribosomal RNA (16SrRNA) gene containing a DNA comprising the base sequence of SEQ ID NO: 1, or an isolated  Paenibacillus polymyxa  bacterium deposited at the ATCC® under the terms of the Budapest Treaty and designated Accession Number PTA-10436, in a cell culture medium to produce a cell culture supernatant comprising the anti-microbial agent. 
     
     
         15 . The method of  claim 14 , further comprising isolating or purifying the anti-microbial agent from the cell culture supernatant. 
     
     
         16 . A method of producing an anti-microbial agent comprising growing or culturing an isolated  Paenibacillus polymyxa  bacterium which has a 16S ribosomal RNA (16SrRNA) gene containing a DNA comprising the base sequence of SEQ ID NO: 1, or an isolated  Paenibacillus polymyxa  bacterium deposited at the ATCC® under the terms of the Budapest Treaty and designated Accession Number PTA-10436, in a cell culture medium to produce a cell culture comprising the anti-microbial agent. 
     
     
         17 . The method of  claim 16 , further comprising isolating or purifying the anti-microbial agent from the cell culture. 
     
     
         18 . A method of reducing mortality or morbidity of an animal comprising administering an effective amount of an anti-microbial composition to the animal, wherein the anti-microbial composition comprises:
 (a) an isolated  Paenibacillus polymyxa  bacterium which has a 16S ribosomal RNA (16SrRNA) gene containing a DNA comprising the base sequence of SEQ ID NO: 1;   (b) an isolated  Paenibacillus polymyxa  bacterium deposited at the ATCC® under the terms of the Budapest Treaty and designated Accession Number PTA-10436;   (c) a cell culture comprising the  Paenibacillus polymyxa  bacterium;   (d) a cell culture supernatant derived from growing the  Paenibacillus polymyxa  bacterium in a cell culture medium; or   (e) an anti-microbial agent isolated from the  Paenibacillus polymyxa  bacterium, the cell culture or the cell culture supernatant.   
     
     
         19 . The method of  claim 18 , wherein the animal is a human, a pet or an agricultural animal. 
     
     
         20 . The method of  claim 18 , wherein the anti-microbial composition is used as a probiotic or a prebiotic.