Oxyfluorfen resistant rice lines

Abstract

The present invention is directed toward rice lines containing mutant allele ROXY that exhibit non-transgenic resistance to the herbicide oxyfluorfen. The invention relates to the seeds, plants and plant parts of rice plants containing mutant allele ROXY and to methods for producing a rice plant by crossing rice plants containing mutant allele ROXY with itself or another rice cultivar. The invention further relates to methods for producing a rice plant containing mutant allele ROXY containing in its genetic material one or more transgenes and to the transgenic rice plants and plant parts produced by those methods. This invention also relates to rice cultivars or breeding cultivars and plant parts derived from rice plants containing mutant allele ROXY, to methods for producing other rice cultivars, lines or plant parts derived from rice plants containing mutant allele ROXY and to the rice plants, varieties, and their parts derived from the use of those methods. The invention further relates to transferring mutant allele ROXY to different genetic backgrounds.

1 . A rice plant having non-transgenic resistance to the herbicide oxyfluorfen. 
     
     
         2 . A rice plant, a plant part thereof, or a rice seed having non-transgenic resistance to the herbicide oxyfluorfen, wherein said non-transgenic resistance to oxyfluorfen is conferred by mutant allele ROXY. 
     
     
         3 . A rice plant, a plant part thereof, or a rice seed having non-transgenic resistance to the herbicide oxyfluorfen conferred by mutant allele ROXY, wherein a representative sample of seed containing said mutant allele ROXY was deposited under ATCC Accession No. PTA-123525. 
     
     
         4 . A tissue culture of cells or protoplasts produced from the plant of  claim 3 , wherein said cells or protoplasts of the tissue culture are produced from a plant part selected from the group consisting of leaf, pollen, embryo, cotyledon, hypocotyl, meristematic cell, root, root tip, pistil, anther, flower, stem, glume and panicle. 
     
     
         5 . A rice plant regenerated from the tissue culture of  claim 4 , wherein the plant contains mutant allele ROXY. 
     
     
         6 . A method for producing an F 1  hybrid rice seed containing mutant allele ROXY, wherein the method comprises crossing the plant of  claim 3  with a different rice plant and harvesting the resultant F 1  hybrid rice seed. 
     
     
         7 . A hybrid rice seed produced by the method of  claim 6 . 
     
     
         8 . A hybrid rice plant, or a plant part thereof, produced by growing said hybrid seed of  claim 7 , wherein said plant or plant part thereof contains mutant allele ROXY. 
     
     
         9 . A method of producing an herbicide resistant rice plant wherein the method comprises transforming the rice plant of  claim 3  with a transgene wherein the transgene confers resistance to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine, benzonitrile and acetyl CoA carboxylase inhibitors. 
     
     
         10 . An herbicide resistant rice plant produced by the method of  claim 9 . 
     
     
         11 . A method of producing an insect resistant rice plant wherein the method comprises transforming the rice plant of  claim 3  with a transgene that confers insect resistance. 
     
     
         12 . An insect resistant rice plant produced by the method of  claim 11 . 
     
     
         13 . The rice plant of  claim 12 , wherein the transgene encodes a  Bacillus thuringiensis  endotoxin. 
     
     
         14 . A method of producing a disease resistant rice plant wherein the method comprises transforming the rice plant of  claim 3  with a transgene that confers disease resistance. 
     
     
         15 . A disease resistant rice plant produced by the method of  claim 14 . 
     
     
         16 . A method of producing a rice plant with modified fatty acid metabolism or modified carbohydrate metabolism wherein the method comprises transforming the rice plant of  claim 3  with a transgene encoding a protein selected from the group consisting of fructosyltransferase, levansucrase, alpha-amylase, invertase and starch branching enzyme or DNA encoding an antisense of stearyl-ACP desaturase. 
     
     
         17 . A rice plant having modified fatty acid metabolism or modified carbohydrate metabolism produced by the method of  claim 16 . 
     
     
         18 . A method of producing a genetically modified rice plant, wherein the method comprises genome editing of the plant of  claim 3 . 
     
     
         19 . A genetically modified rice plant produced by the method of  claim 18 . 
     
     
         20 . A method of introducing a desired trait into a rice plant having non-transgenic resistance to the herbicide oxyfluorfen conferred by mutant allele ROXY wherein the method comprises:
 (a) crossing a rice plant having non-transgenic resistance to the herbicide oxyfluorfen conferred by mutant allele ROXY, wherein a representative sample of seed containing mutant allele ROXY was deposited under ATCC Accession No. PTA-123525, with a plant of another rice cultivar that comprises a desired trait to produce progeny plants wherein the desired trait is selected from the group consisting of male sterility, herbicide resistance, insect resistance, modified fatty acid metabolism, modified carbohydrate metabolism and resistance to bacterial disease, fungal disease or viral disease;   (b) selecting one or more progeny plants that have the desired trait to produce selected progeny plants;   (c) backcrossing the selected progeny plants with the rice plant containing mutant allele ROXY to produce backcross progeny plants;   (d) selecting for backcross progeny plants that have the desired trait and contain mutant allele ROXY; and   (e) repeating steps (c) and (d) two or more times to produce selected third or higher backcross progeny plants that comprise the desired trait.   
     
     
         21 . A plant produced by the method of  claim 20 , wherein the plant has the desired trait and contains mutant allele ROXY. 
     
     
         22 . The plant of  claim 21 , wherein the desired trait is herbicide resistance and the resistance is conferred to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine, benzonitrile and acetyl CoA carboxylase inhibitors. 
     
     
         23 . The plant of  claim 21 , wherein the desired trait is insect resistance and the insect resistance is conferred by a transgene encoding a  Bacillus thuringiensis  endotoxin. 
     
     
         24 . The plant of  claim 21 , wherein the desired trait is modified fatty acid metabolism or modified carbohydrate metabolism and said desired trait is conferred by a nucleic acid encoding a protein selected from the group consisting of fructosyltransferase, levansucrase, alpha-amylase, invertase and starch branching enzyme or DNA encoding an antisense of stearyl-ACP desaturase. 
     
     
         25 . A method of transferring mutant allele ROXY to a different genetic background, wherein the method comprises:
 (a) obtaining the F 1  plant of  claim 8 ;   (b) backcrossing said F 1  plant to a recipient parent plant not having mutant allele ROXY to produce backcross progeny plants;   (c) selecting for backcross progeny plants that contain mutant allele ROXY;   (d) backcrossing said selected backcross progeny plants to said recipient parent;   (e) repeating steps (c) and (d) two or more times in succession to produce selected third or higher backcross progeny plants that contain mutant allele ROXY; and harvesting the resultant seed.   
     
     
         26 . A plant produced from the seed of  claim 25 , wherein said plant contains mutant allele ROXY. 
     
     
         27 . A method for controlling or suppressing weeds at a rice production site, the method comprising the steps of:
 (a) providing, at said site, a rice plant, plant part thereof, or rice seed of  claim 2  containing mutant allele ROXY; and   (b) applying to said site an amount of herbicide effective to control or suppress weeds.   
     
     
         28 . A method for growing a plant of  claim 2  while controlling or suppressing weeds in the vicinity of said plant, said method comprising the steps of:
 (a) growing said plant; and 
 (b) applying herbicide to said plant and weeds at a level of herbicide that would inhibit the growth of a corresponding rice plant not containing mutant allele ROXY. 
 
     
     
         29 . A method for controlling or suppressing weeds at a rice production site, the method comprising the steps of:
 (a) applying to said site an amount of herbicide effective to control or suppress weeds; and   (b) providing, at said site, the rice plant, plant part thereof, or rice seed of  claim 2  containing mutant allele ROXY.   
     
     
         30 . A method for growing a plant of  claim 2  while controlling or suppressing weeds in the vicinity of said plant, said method comprising the steps of:
 (a) applying herbicide in the vicinity where said plant will grow, at a level of herbicide that would inhibit the growth of a corresponding rice plant not containing mutant allele ROXY; and 
 (b) growing said plant. 
 
     
     
         31 . The rice plant, plant part thereof, or rice seed of  claim 2 , wherein said rice plant, plant part thereof, or rice seed has resistance to PPO inhibiting herbicides, herbicide mixtures or herbicide combinations with oxyfluorfen. 
     
     
         32 . The rice plant, plant part thereof, or rice seed of  claim 31 , wherein said herbicide mixtures or herbicides used in combination with oxyfluorfen are selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine, benzonitrile and acetyl CoA carboxylase inhibitors.