US2018019412A1PendingUtilityA1
Compositions Comprising A Polypeptide Having Cellulolytic Enhancing Activity And A Quinone Compound And Uses Thereof
Est. expiryAug 12, 2030(~4 yrs left)· nominal 20-yr term from priority
C07D 495/04Y02E10/549H01L 51/424H01L 51/0068H01L 51/0074H10K 85/6576H10K 85/621H10K 2102/103H10K 30/152H10K 85/655H10K 85/113Y02E50/10C07D 333/72C07D 333/76C07D 333/52C12N 9/96C12P 7/40C12P 5/02C12P 19/02C12P 7/10C12P 7/28C12Y 302/01004C12P 2203/00C07K 14/385C12P 2201/00C12P 19/14C12P 5/002C12N 9/2437C12Y 302/01021C12N 9/2445C07K 14/38C12P 19/12C12P 7/26C12P 13/04C07K 14/37C12P 5/026
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Claims
Abstract
The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a quinone compound. The present invention also relates to methods of using the compositions.
Claims
exact text as granted — not AI-modified1 - 15 . (canceled)
16 . An enzyme composition comprising: (a) a GH61 polypeptide having cellulolytic enhancing activity and (b) a quinone compound, wherein the combination of the GH61 polypeptide having cellulolytic enhancing activity and the quinone compound enhances hydrolysis of a cellulosic material by the enzyme composition compared to the GH61 polypeptide alone or the quinone compound alone according to the following formula with a ratio greater than 1:
GH
61
effect
=
%
conversion
(
+
GH
61
+
quinone
compound
)
%
conversion
(
no
GH
61
+
quinone
compound
)
;
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation.
17 . The enzyme composition of claim 16 , wherein the quinone compound is a compound of formula (I) or (II):
wherein R 1 , R 2 , R 3 , and R 4 are independently hydrogen, halogen, —OH, —OR 8 , —NO 2 , —N(R)(R 10 ), —CN, —C(O)R 5 , —C(O)OR 6 , —C(O)NHR 7 , —OC(O)R 11 , —NHC(O)R 12 , —OC(O)OR 13 , —NHC(O)OR 14 , —OC(O)NHR 15 , —NHC(O)NHR 16 , —SO 2 R 17 , —SO 2 N(R 18 )(R 19 ), —SR 20 , —NC(R 21 )(R 22 ), or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , R 12 , R 13 , R 14 , R 15 , R 16 , R 18 , R 19 , R 20 , R 21 , and R 22 are independently hydrogen, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl; and
R 17 is an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl; and
wherein each pair of R 1 and R 2 , R 2 and R 3 , and R 3 and R 4 may combine to form an optionally substituted fused ring;
or a salt or solvate thereof.
18 . The enzyme composition of claim 17 , wherein the quinone compound is selected from the group consisting of: (I-1): 1,4-benzoquinone; (I-2): 1,4-naphthoquinone; (I-3): 2-hydroxy-1,4-naphthoquinone; (I-4): 2,3-dimethoxy-5-methyl-1,4-benzoquinone or coenzyme Q 0 ; (I-5): 2,3,5,6-tetramethyl-1,4-benzoquinone or duroquinone; (I-6): 1,4-dihydroxyanthraquinone; (II-1): 3-hydroxy-1-methyl-5,6-indolinedione or adrenochrome; (II-2): 4-tert-butyl-5-methoxy-1,2-benzoquinone; and (11-3): pyrroloquinoline quinone; or a salt or solvate thereof.
19 . The enzyme composition of claim 16 , wherein the enzyme composition further comprises one or more enzymes selected from the group consisting of a cellulase, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.
20 . The enzyme composition of claim 19 , wherein the cellulase is one or more enzymes selected from the group consisting of an endoglucanase, a cellobiohydrolase, and a beta-glucosidase.
21 . The enzyme composition of claim 19 , wherein the hemicellulase is one or more enzymes selected from the group consisting of a xylanase, an acetyxylan esterase, a feruloyl esterase, an arabinofuranosidase, a xylosidase, and a glucuronidase.
22 . The enzyme composition of claim 16 , wherein an effective amount of the quinone compound to cellulosic material as a molar ratio to glucosyl units of cellulose is about 10 −6 to about 10.
23 . A method of fermenting a cellulosic material, comprising: fermenting the cellulosic material with one or more fermenting microorganisms, wherein the cellulosic material is saccharified with the enzyme composition of claim 16 .
24 . The method of claim 23 , wherein the quinone compound is a compound of formula (I) or (II):
wherein R 1 , R 2 , R 3 , and R 4 are independently hydrogen, halogen, —OH, —OR 8 , —NO 2 , —N(R)(R 10 ), —CN, —C(O)R 5 , —C(O)OR 6 , —C(O)NHR 7 , —OC(O)R 11 , —NHC(O)R 12 , —OC(O)OR 13 , —NHC(O)OR 14 , —OC(O)NHR 15 , —NHC(O)NHR 10 , —SO 2 R 17 , —SO 2 N(R 18 )(R 19 ), —SR 20 , —NC(R 21 )(R 22 ), or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , R 12 , R 13 , R 14 , R 15 , R 16 , R 18 , R 10 , R 20 , R 21 , and R 22 are independently hydrogen, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl; and
R 17 is an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl; and
wherein each pair of R 1 and R 2 , R 2 and R 3 , and R 3 and R 4 may combine to form an optionally substituted fused ring;
or a salt or solvate thereof.
25 . The method of claim 24 , wherein the quinone compound is selected from the group consisting of: (I-1): 1,4-benzoquinone; (I-2): 1,4-naphthoquinone; (I-3): 2-hydroxy-1,4-naphthoquinone; (I-4): 2,3-dimethoxy-5-methyl-1,4-benzoquinone or coenzyme Q 0 ; (I-5): 2,3,5,6-tetramethyl-1,4-benzoquinone or duroquinone; (I-6): 1,4-dihydroxyanthraquinone; (II-1): 3-hydroxy-1-methyl-5,6-indolinedione or adrenochrome; (II-2): 4-tert-butyl-5-methoxy-1,2-benzoquinone; and (11-3): pyrroloquinoline quinone; or a salt or solvate thereof.
26 . The method of claim 23 , wherein the cellulosic material is pretreated
27 . The method of claim 23 , wherein the enzyme composition further comprises one or more enzymes selected from the group consisting of a cellulase, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.
28 . The enzyme composition of claim 27 , wherein the cellulase is one or more enzymes selected from the group consisting of an endoglucanase, a cellobiohydrolase, and a beta-glucosidase.
29 . The enzyme composition of claim 27 , wherein the hemicellulase is one or more enzymes selected from the group consisting of a xylanase, an acetyxylan esterase, a feruloyl esterase, an arabinofuranosidase, a xylosidase, and a glucuronidase.
30 . The method of claim 23 , wherein the fermenting of the cellulosic material produces a fermentation product.
31 . The method of claim 30 , further comprising recovering the fermentation product from the fermentation.
32 . The method of claim 31 , wherein the fermentation product is an alcohol, an alkane, a cycloalkane, an alkene, an amino acid, a gas, isoprene, a ketone, an organic acid, or polyketide.
33 . The method of claim 23 , wherein an effective amount of the quinone compound to cellulosic material as a molar ratio to glucosyl units of cellulose is about 10 −6 to about 10.Join the waitlist — get patent alerts
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