Polypeptides Having Laccase Activity and Polynucleotides Encoding Same

Abstract

The present invention relates to isolated polypeptides having laccase activity and polynucleotides encoding the polypeptides and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

1 - 11 . (canceled) 
     
     
         12 . A process for degrading a cellulosic material, comprising: treating the cellulosic material with an enzyme composition in the presence of the polypeptide having laccase activity selected from the group consisting of:
 (a) a polypeptide having at least 60% sequence identity to the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO:18, or SEQ ID NO: 22; at least 65% sequence identity to the mature polypeptide of SEQ ID NO: 34 or SEQ ID NO: 36; at least 70% sequence identity to the mature polypeptide of SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 26, SEQ ID NO: 30, or SEQ ID NO: 32; at least 75% sequence identity to the mature polypeptide of SEQ ID NO: 28; or at least 85% sequence identity to the mature polypeptide of SEQ ID NO: 4;   (b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium-high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO:17, SEQ ID NO: 21, SEQ ID NO: 33, or SEQ ID NO: 35, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii); or at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, or SEQ ID NO: 31, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii); or at least very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 3, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);   (c) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO:17, or SEQ ID NO: 21, or the cDNA sequences thereof; at least 65% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 33 or SEQ ID NO: 35, or the cDNA sequences thereof; at least 70% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 25, SEQ ID NO: 29, or SEQ ID NO: 31, or the cDNA sequences thereof; at least 75% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 27 or the cDNA sequence thereof; or at least 85% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 3 or the cDNA sequence thereof;   (d) a variant of the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO: 30, SEQ ID NO: 32, SEQ ID NO: 34, or SEQ ID NO: 36 comprising a substitution, deletion, and/or insertion at one or more (e.g., several) positions; and   (e) a fragment of the polypeptide of (a), (b), (c), or (d) that has laccase activity.   
     
     
         13 - 21 . (canceled) 
     
     
         22 . The process of  claim 12 , wherein the polypeptide having laccase activity comprises or consists of the mature polypeptide of comprising or consisting of the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO: 30, SEQ ID NO: 32, SEQ ID NO: 34, or SEQ ID NO: 36. 
     
     
         23 . The process of  claim 22 , wherein the mature polypeptide is amino acids 21 to 591 of SEQ ID NO: 2, amino acids 26 to 610 of SEQ ID NO: 4, amino acids 20 to 585 of SEQ ID NO: 6, amino acids 24 to 590 of SEQ ID NO: 8, amino acids 20 to 617 of SEQ ID NO: 10, amino acids 24 to 576 of SEQ ID NO: 12, amino acids 22 to 606 of SEQ ID NO: 14, amino acids 17 to 559 of SEQ ID NO: 16, amino acids 24 to 603 of SEQ ID NO: 18, amino acids 21 to 581 of SEQ ID NO: 20, amino acids 20 to 596 of SEQ ID NO: 22, amino acids 22 to 593 of SEQ ID NO: 26, amino acids 23 to 584 of SEQ ID NO: 28, amino acids 23 to 606 of SEQ ID NO: 30, amino acids 22 to 619 of SEQ ID NO: 32, amino acids 18 to 585 of SEQ ID NO: 34, amino acids 22 to 604 of SEQ ID NO: 36.