Polypeptides Having N-Acetyl Glucosamine Oxidase Activity

Abstract

The present invention relates to polypeptides having N-acetyl glucosamine oxidase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

1 - 15 . (canceled) 
     
     
         16 . A polypeptide having N-acetyl-D-glucosamine oxidase activity, selected from the group consisting of:
 (a) a polypeptide having at least 75% sequence identity to the mature polypeptide of SEQ ID NO: 2;   (b) a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);   (c) a polypeptide encoded by a polynucleotide having at least 75% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof; and   (d) a fragment of the polypeptide of (a), (b), or (c) that has N-acetyl-D-glucosamine oxidase activity.   
     
     
         17 . The polypeptide of  claim 16 , comprising or consisting of SEQ ID NO: 2 or the mature polypeptide of SEQ ID NO: 2. 
     
     
         18 . The polypeptide of  claim 16 , wherein the mature polypeptide is amino acids 20 to 632 of SEQ ID NO: 2. 
     
     
         19 . The polypeptide of  claim 16 , which has at least 20% of the antimicrobial activity of the mature polypeptide of SEQ ID NO: 2. 
     
     
         20 . The polypeptide of  claim 19 , wherein the antimicrobial activity is assessed using a method described in Example 6, 7, 8, or 9. 
     
     
         21 . The polypeptide of  claim 19 , wherein the antimicrobial activity is assessed as fungicidal or sporicidal effect towards any of the fungal strains  Aspergillus carbonarius  BR00732 (CBS 139193) and  Aspergillus niger  strain BR00883 (accession number CBS 139194). 
     
     
         22 . A cleaning composition consisting of the polypeptide of  claim 16  and one or more ingredients used in cleaning compositions, such as a surfactant. 
     
     
         22 . A method of cleaning and/or disinfecting comprising applying the composition of  claim 21 . 
     
     
         24 . The method of  claim 22 , which is cleaning-in-place. 
     
     
         25 . A polynucleotide encoding the polypeptide of  claim 16 . 
     
     
         26 . A nucleic acid construct or expression vector comprising the polynucleotide of  claim 25 , wherein the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in a host cell. 
     
     
         27 . A recombinant host cell comprising the polynucleotide of  claim 25 , wherein the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in the host cell. 
     
     
         28 . A method of producing a polypeptide having N-acetyl-D-glucosamine oxidase activity, comprising cultivating the host cell of  claim 27  under conditions conducive for production of the polypeptide. 
     
     
         29 . The method of  claim 28 , further comprising recovering the polypeptide.