Tissue transplantation involving, and tissues modified by decellularization and recellularization of donor tissues for minimized or obviated rejection reactions

Abstract

The method of tissue transplantation involving recellularizing of a donor organ with the utilization of the recipient's cytokines collected from the recipient's blood plasma at less than systemic pressure, and at the temperature greater than freezing and less than normal systemic temperature of the recipient's blood. Specifically, a method of harvesting a platelet-derived growth factors from platelet rich plasma (PRP), the growth factors having increased weight.

We claim: 
     
         1 . A method for adapting tissue for trans-organism implantation comprising the steps of:
 selecting donor tissue from a first organism;   decellularizing said donor tissue;   selecting stem cells and cytokines from a second, recipient organism;   infusing said donor tissue with said stem cells and said cytokines;   implanting said donor tissue in said second, recipient organism; and   injecting autologous plasma into said second, recipient organism.   
     
     
         2 . The method of  claim 1  wherein said trans-organism transplantation involves donor tissue from a said first organism of a first species and said second organism is from the same species. 
     
     
         3 . The method of  claim 1  wherein said trans-organism transplantation involves donor tissue from a said first organism of a first species and said second organism is from a second species. 
     
     
         4 . The method of  claim 1  wherein said donor tissue is an organ 
     
     
         5 . The method of  claim 4  wherein said donor tissue is a kidney. 
     
     
         6 . The method of  claim 3  wherein said donor tissue is an organ. 
     
     
         7 . The method of  claim 6  wherein said donor tissue is a kidney 
     
     
         8 . The method of  claim 1  wherein said cytokines being extracted is performed when said blood of said second organism is at a pressure less than ambient pressure. 
     
     
         9 . The method of  claim 1  wherein said cytokines being extracted is performed when said blood of said second organism is at a temperature greater than freezing and less than normal systemic temperature of said second organism. 
     
     
         10 . Modified tissue for trans-organism implantation produced through a process comprising the steps of:
 selecting donor tissue from a first organism;   decellularizing said donor tissue;   selecting stem cells and cytokines from a second, recipient organism;   infusing said donor tissue with said stem cells and said cytokines;   implanting said donor tissue in said second, recipient organism; and   injecting autologous plasma into said second, recipient organism.   
     
     
         11 . The modified tissue of of  claim 10  wherein said trans-organism transplantation involves donor tissue from a said first organism of a first species and said second organism is from the same species. 
     
     
         12 . The modified tissue of of  claim 10  wherein said trans-organism transplantation involves donor tissue from a said first organism of a first species and said second organism is from a second species. 
     
     
         13 . The modified tissue of of  claim 10  wherein said donor tissue is an organ 
     
     
         14 . The modified tissue of of  claim 12  wherein said donor tissue is a kidney. 
     
     
         15 . The modified tissue of of  claim 12  wherein said donor tissue is an organ. 
     
     
         16 . The modified tissue of of  claim 15  wherein said donor tissue is a kidney 
     
     
         17 . The modified tissue of of  claim 10  wherein said cytokines being extracted is performed when said blood of said second organism is at a pressure less than ambient pressure. 
     
     
         18 . The modified tissue of of  claim 10  wherein said cytokines being extracted is performed when said blood of said second organism is at a temperature greater than freezing and less than normal systemic temperature of said second organism.