Bacillus Sp. Biosurfactants, Composition Including Same, Method for Obtaining Same, and Use Thereof

Abstract

Biosurfactants produced by a strain of Bacillus sp and to uses thereof. A composition comprising the biosurfactants, and a method for producing the biosurfactants. A method for obtaining a biosurfactant, and a device for implementing the method. The production of biopesticides or biosurfactants for the phytosanitary industry, and in the fields of the food, cosmetics pharmaceutical and oil industries and the environment.

1 - 15 . (canceled) 
     
     
         16 . A population of genetically engineered  Bacillus subtilis  bacteria, wherein the promoter of the myc operon has been replaced by a constitutive promoter, and wherein the bacteria produce a mycosubtilin, the fatty acid chain of which comprises 17 carbon atoms and which has glutamine in place of asparagine in position 3 in its peptide cycle (C 17  Gln3 mycosubtilin), represented by the following formula (II): 
       
         
           
           
               
               
           
         
       
     
     
         17 . The population of genetically engineered  Bacillus subtilis  bacteria of  claim 16 , wherein the constitutive promoter is P repU . 
     
     
         18 . The population of genetically engineered  Bacillus subtilis  bacteria of  claim 16 , wherein the bacteria comprise a srfA operon that has been interrupted. 
     
     
         19 . The population of genetically engineered  Bacillus subtilis  bacteria of  claim 18 , which does not produce surfactin. 
     
     
         20 . A method of producing a population of genetically engineered  Bacillus subtilis  bacteria, the method comprising providing a parental strain of  Bacillus subtilis  bacteria, interrupting the srfA operon, and replacing the promoter of the myc operon with a constitutive promoter, such that the engineered bacteria does not produce any surfactin and produces a larger quantity of mycosubtilins as compared to the parental strain. 
     
     
         21 . The method of  claim 20 , wherein the parental strain of  Bacillus subtilis  bacteria is ATCC 6633. 
     
     
         22 . The method of  claim 20 , wherein the engineered bacteria produces C 17  Gln3 mycosubtilin. 
     
     
         23 . A population of genetically engineered  Bacillus subtilis  bacteria produced by the method of  claim 20 . 
     
     
         24 . A  Bacillus subtilis  bacteria strain BBG125, which was deposited in the National Collection of Microorganism Cultures (CNCM) under number CNCM I-4451. 
     
     
         25 . A method of producing a mycosubtilin composition, the method comprising:
 providing the population of genetically engineered  Bacillus subtilis  bacteria of  claim 16 ;   culturing the bacteria in a culture medium comprising an organic substrate to produce a mycosubtilin composition; and   recovering the mycosubtilin composition.   
     
     
         26 . The method of  claim 25 , wherein the mycosubtilin composition comprises 1%-20% C 17  Gln3 mycosubtilin. 
     
     
         27 . The method of  claim 26 , further comprising assaying for the presence of 1%-20% C 17  Gln3 mycosubtilin. 
     
     
         28 . The method of  claim 25 , wherein the organic substrate comprises starch, glucose, saccharose, glycerol, organic acids, amino acids, or a mixture thereof. 
     
     
         29 . The method of  claim 25 , wherein the organic substrate does not comprise xylose.