US2017349885A1PendingUtilityA1

Protein complex capable of catalyzing asymmetric oxidation reaction and method for producing same

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Assignee: SANYO FOODS CO LTDPriority: Nov 9, 2012Filed: Jun 12, 2017Published: Dec 7, 2017
Est. expiryNov 9, 2032(~6.3 yrs left)· nominal 20-yr term from priority
Inventors:Hiroyuki Nagaoka
C12N 9/0065C12P 41/002C12N 9/0053C12N 9/0006C12P 7/22C12Y 111/01C12Y 109/03001C12Y 101/05
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Claims

Abstract

Provided are: a protein complex capable of selectively and asymmetrically oxidizing an enantiomer of a secondary alcohol without adding a coenzyme and having an asymmetric oxidation activity in a water-soluble solvent system in the presence of oxygen; a method for producing the same; and a method for coating the protein complex with a high molecular weight compound. The method for producing the protein complex includes: (1) enclosing a crude water-soluble protein in a gel, air-oxidizing the gel, and eluting the protein complex into an aqueous solution; and (2) applying gravity to concentrate and precipitate the protein complex, redissolving the precipitate in an aqueous glycine sodium hydroxide solution of about 0.5 mM and allowing the same to homogeneously coexist with a high molecular weight compound, and re-precipitating the solution and dehydrating and drying the same to yield a protein complex coated with a high molecular weight compound.

Claims

exact text as granted — not AI-modified
1 - 21 . (canceled) 
     
     
         22 . A process for producing an optically active alcohol, comprising: selectively obtaining one enantiomer of a racemic alcohol by allowing an iron-containing protein complex derived from an animal or plant, which has reactivity with hydrogen peroxide even in the absence of NAD(H) and/or NADP(H), to act on a substrate in the form of the racemic alcohol. 
     
     
         23 . The process for producing an optically active alcohol according to  claim 22 , wherein the other enantiomer not involved in the reaction is selectively obtained by selectively asymmetrically oxidizing the one enantiomer of the racemic alcohol to a ketone. 
     
     
         24 . The process for producing an optically active alcohol according to  claim 23 , wherein the protein complex is coated with a polymer compound and water is used for the solvent in the reaction system. 
     
     
         25 . The process for producing an optically active alcohol according to  claim 24 , wherein the protein complex is subjected to chemical modification treatment, and glycine-NaOH buffer (50 mM, pH 9.0) is used for the solvent in the reaction system. 
     
     
         26 . The process for producing an optically active alcohol according to  claim 22 , wherein catalyst activity of the protein complex is not impaired even in the case of using the protein complex coated with a polymer compound and using any solvent selected from the group consisting of DMSO, IPA, ethanol and water as a cosolvent in the reaction system. 
     
     
         27 . The process for producing an optically active alcohol according to  claim 24 , wherein the protein complex is subjected to chemical modification treatment, and DMSO (<2% (v/v)) is used as a cosolvent in the reaction system. 
     
     
         28 . The process for producing an optically active alcohol according to  claim 22 , wherein a catalytic reaction is carried out in an uncapped or unsealed mild oxygen supply system. 
     
     
         29 . The process for producing an optically active alcohol according to  claim 22 , wherein a catalyst and/or protein complex in the reaction system carries out an asymmetric oxidation reaction accompanying an iron and oxygen electron transfer system instead of an asymmetric oxidation/reduction reaction accompanying a zinc and NAD(P) electron transfer system. 
     
     
         30 - 40 . (canceled) 
     
     
         41 . The process for producing an optically active alcohol according to  claim 24 , wherein the polymer compound has a molecular weight of 4000 or more. 
     
     
         42 . The process for producing an optically active alcohol according to  claim 24 , wherein the polymer compound is polyethylene glycol. 
     
     
         43 . The process for producing an optically active alcohol according to  claim 24 , wherein the polymer compound is a mixture of polymer compounds having different molecular weights, and
 the mixture is composed of a polymer compound having a molecular weight of 4000 or more and a polymer compound having a molecular weight of 1000 or less, and contains one-third or less of the low molecular weight polymer compound.   
     
     
         44 . The process for producing an optically active alcohol according to  claim 43 , wherein the polymer compound is polyethylene glycol.

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