Methods of treating cancer

Abstract

This invention relates to methods of treating cancer in a subject in need thereof, e.g., in a human in need thereof, comprising determining at least one of the following in a sample from said human: (a) the presence or absence of a mutation at the alanine 677 (A677) residue in EZH2 in a sample from said human; or (b) the presence or absence of a mutation at the tyrosine 641 (Y641) residue in EZH2; or (c) the presence or absence of an increased level of H3K27me3 as compared to a control, and administering to said human an effective amount of an EZH2 inhibitor or a pharmaceutically acceptable salt thereof if at least one of said A677 mutation, Y641 mutation, or increased level of H3K27me3 is present in said sample

What is claimed is: 
     
         1 . A kit for the treatment of cancer comprising a kit for determining one or more of the following in a sample from a human:
 a. the presence or absence of a mutation at the alanine 677 (A677) residue in EZH2 Enhancer of Zeste Homolog 2 (EZH2) in a sample from said human; or   b. the presence or absence of a mutation at the tyrosine 641 (Y641) residue in EZH2; or   c. the presence or absence of an increased level of H3K27me3 as compared to a control,   and a means for determining one or more of a, b, and c.   
     
     
         2 . The kit of  claim 1 , wherein said means is selected from the group consisting of primers, probes, and antibodies.