Chloroplast Expressing Colostrum or Milk Polypeptides

Abstract

Provided are chloroplasts engineered to recombinantly express mammalian colostrum and milk polypeptides photosynthetic organisms containing such chloroplasts, and compositions comprising such organisms and methods for producing such organisms. In certain embodiments, provided is a chloroplast comprising one or more polynucleotides encoding one or more mammalian milk or colostrum polypeptides selected from osteopontin, lactadherin, cathelicidin-1, lysozyme, lactoperoxidase, lingual antimicrobial peptide (LAP), alpha-lactalbumin, and soluble CD14.

1 - 73 . (canceled) 
     
     
         74 . A microalgal chloroplast comprising a polynucleotide encoding mammalian lactadherin integrated into the chloroplast genome, wherein the lactadherin polypeptide comprises at least about 85%, 90%, 95%, 98% or 99% sequence identity to SEQ ID NO:10, wherein the chloroplast expresses a bioactive lactadherin polypeptide that is not glycosylated. 
     
     
         75 . The chloroplast of  claim 74 , wherein the chloroplast is from a green algae. 
     
     
         76 . The chloroplast of  claim 75 , wherein the green algae is selected from the group consisting of  Chlamydomonas, Dunaliella, Haematococcus, Chlorella,  and  Scenedesmaceae.    
     
     
         77 . The chloroplast of  claim 74 , wherein the microalgal chloroplast is from a microalga selected from the group consisting of  Chlamydomonas reinhartdii, Dunaliella salina, Haematococcus pluvialis, Chlorella vulgaris, Acutodesmus obliquus, and Scenedesmus dimorphus.    
     
     
         78 . The chloroplast of  claim 74 , wherein the lactadherin polypeptide is from a mammal selected from the group consisting of human, canine, feline, bovine, porcine, ovine, and caprine. 
     
     
         79 . The chloroplast of  claim 74 , wherein the polynucleotide encoding mammalian lactadherin comprises a polynucleotide having at least about 85%, 90%, 95%, 98% or 99% sequence identity sequence identity to SEQ ID NO:9. 
     
     
         80 . A cell comprising the chloroplast of  claim 74 . 
     
     
         81 . The cell of  claim 80 , wherein the cell is intact. 
     
     
         82 . The cell of  claim 80 , wherein the cell is dried. 
     
     
         83 . The cell of  claim 82 , wherein the cell is freeze-dried. 
     
     
         84 . The cell of  claim 80 , wherein the cell is a microalgal cell. 
     
     
         85 . The cell of  claim 80 , wherein the cell is from a green algae selected from the group consisting of  Chlamydomonas, Dunaliella, Haematococcus, Chlorella,  and  Scenedesmaceae.    
     
     
         86 . A method for producing one or more mammalian colostrum or milk proteins, comprising culturing the cell of  claim 80 . 
     
     
         87 . A composition edible by a mammal and/or a chicken comprising one or more populations of cells of  claim 80 . 
     
     
         88 . The composition of  claim 87 , wherein the composition is selected from a beverage, a food, a feed, a food supplement, and a nutraceutical. 
     
     
         89 . The composition of  claim 87 , wherein the composition is selected from the group consisting of a compressed algal cake, an algal paste, and an algal powder. 
     
     
         90 . The composition of  claim 87 , wherein the composition is dried. 
     
     
         91 . The composition of  claim 90 , wherein the composition is freeze-dried, lyophilized, or spray-dried.