Methods for cellobiosan utilization

Abstract

Disclosed herein are enzymes useful for the degradation of cellobiosan in materials such a pyrolysis oils. Methods of degrading cellobiosan using enzymes or organisms expressing the same are also disclosed.

1 - 20 . (canceled) 
     
     
         21 . A method for degrading cellobiosan, comprising contacting a cellobiosan containing material with a microbial β-glucosidase. 
     
     
         22 . The method of  claim 21 , wherein the (β-glucosidase is from a bacterium or a fungus. 
     
     
         23 . The method of  claim 22 , wherein the (β-glucosidase is from a bacterium. 
     
     
         24 . The method of  claim 23 , wherein the β-glucosidase comprises a Glycoside Hydrolase Family 1 (GH1) domain. 
     
     
         25 . The method of  claim 24 , wherein the bacterium is from the genus  Agrobacterium  or the genus  Thermotoga.    
     
     
         26 . The method of  claim 22 , wherein the β-glucosidase is from a fungus. 
     
     
         27 . The method of  claim 26 , wherein the β-glucosidase comprises a Glycoside Hydrolase Family 3 (GH3) domain. 
     
     
         28 . The method of  claim 27 , wherein the fungus is from the genus  Aspergillus  or the genus  Phanerochaete.    
     
     
         29 . The method of  claim 21 , wherein the cellobiosan containing material is a pyrolysis oil obtained from biomass. 
     
     
         30 . The method of  claim 21 , wherein the cellobiosan containing material is a fraction obtained by fractionating a pyrolysis oil. 
     
     
         31 . The method of  claim 30 , wherein the pyrolysis oil fraction is an aqueous fraction. 
     
     
         32 . The method of  claim 21 , wherein the cellobiosan containing material is also contacted with a levoglucosan kinase. 
     
     
         33 . The method of  claim 32 , wherein the levoglucosan kinase is LGK from  Lipomyces starkeyi.    
     
     
         34 . The method of  claim 21 , wherein the β-glucosidase is expressed by a microorganism. 
     
     
         35 . The method of  claim 34 , wherein the microorganism is a bacterium. 
     
     
         36 . The method of  claim 35 , wherein the bacterium is from the genus  Pseudomonas.    
     
     
         37 . The method of  claim 36 , wherein the bacterium is  P. putida.    
     
     
         38 . The method of  claim 34 , wherein the cellobiosan containing material is also contacted with a levoglucosan kinase. 
     
     
         39 . The method of  claim 38 , wherein the levoglucosan kinase is LGK from  Lipomyces starkeyi.    
     
     
         40 . A method for producing levoglucosan and glucose from cellobiosan, comprising:
 a) contacting the cellobiosan with at least one microbial β-glucosidase.   b) isolating at least one of the levoglucosan or glucose products.