Recombinant proteins of parapoxvirus ovis and pharmaceutical compositions therefrom

Abstract

The invention relates to polynucleotides coding for the PPVO viral genome, to fragments of the polynucleotides coding for the PPVO genome and to polynucleotides coding for individual open reading frames (ORFs) of the PPVO viral genome. The invention also relates to recombinant proteins expressed from the above mentioned polynucleotides and to fragments of said recombinant proteins, and to the use of said recombinant proteins or fragments for the preparation of pharmaceutical compositions.

1 . A method for inducing an immunomodulatory activity in a subject, consisting of administering to the subject an individual recombinant protein encoded by a polynucleotide selected from the group consisting of:
 (i) a polynucleotide having the sequence consisting of nucleotide residues 31003 to 46845 of SEQ ID NO:1 (PPVO insert of VVOV 96),   (ii) a polynucleotide having the sequence consisting of nucleotide residues 24056 to 33789 of SEQ ID NO:1 (PPVO insert of VVOV 97),   (iii) a polynucleotide having the sequence consisting of nucleotide residues 82324 to 92502 of SEQ ID NO:1 (PPVO insert of VVOV 243),   (iv) a polynucleotide having the sequence consisting of nucleotide residues 74804 to 88576 of SEQ ID NO:1 (PPVO insert of VVOV 285), and   (v) a polynucleotide having the sequence consisting of nucleotide residues 102490 to 108393 of SEQ ID NO:1 (PPVO insert of VVOV 330).   
     
     
         2 . The method of  claim 1 , wherein the immunomodulatory activity results in the induction of interferon-gamma. 
     
     
         3 . The method of  claim 1 , wherein the immunomodulatory activity results in the induction of tumor necrosis factor-alpha. 
     
     
         4 . The method of  claim 1 , wherein the immunomodulatory activity results in the stimulation of MHC-I cross-presentation. 
     
     
         5 . The method of  claim 1 , wherein the recombinant protein is attached to or is a part of a structure selected from the group consisting of: particle-like structure, fusion protein, protein coated particle, and virus-like particle. 
     
     
         6 . A method for inducing an immunomodulatory activity in a subject, consisting of administering to the subject an individual recombinant virus containing a recombinant protein encoded by a polynucleotide selected from the group consisting of:
 (i) a polynucleotide having the sequence consisting of nucleotide residues 31003 to 46845 of SEQ ID NO:1 (PPVO insert of VVOV 96),   (ii) a polynucleotide having the sequence consisting of nucleotide residues 24056 to 33789 of SEQ ID NO:1 (PPVO insert of VVOV 97),   (iii) a polynucleotide having the sequence consisting of nucleotide residues 82324 to 92502 of SEQ ID NO:1 (PPVO insert of VVOV 243),   (iv) a polynucleotide having the sequence consisting of nucleotide residues 74804 to 88576 of SEQ ID NO:1 (PPVO insert of VVOV 285), and   (v) a polynucleotide having the sequence consisting of nucleotide residues 102490 to 108393 of SEQ ID NO:1 (PPVO insert of VVOV 330).   
     
     
         7 . The method of  claim 6  wherein the immunomodulatory activity results in the induction of interferon-gamma. 
     
     
         8 . The method of  claim 6  wherein the immunomodulatory activity results in the induction of tumor necrosis factor-alpha. 
     
     
         9 . The method of  claim 6  wherein the immunomodulatory activity results in the stimulation of MHC-I cross-presentation.