Cell trapping method, method for producing specific cell- trapping device, and method for producing specific cell-containing solution

Abstract

Provided is a cell trapping method for selectively trapping a specific cell included in a cell-containing solution at a filter surface by filtering a liquid, and the method includes a step of draining a liquid for n (n is a natural number) times, in which from the first step of draining the liquid to the n-th step of draining the liquid, a liquid surface of the liquid in the introduction region on the filter is maintained at a predetermined liquid surface height, and when the n-th step of draining the liquid is completed, the discharging of the liquid from the cell trapping device is stopped in a state where the liquid surface is at a predetermined height in the filter.

1 . A cell trapping method for selectively trapping a specific cell included in a cell-containing solution at a filter surface by filtering a liquid using a cell trapping device, the cell trapping device including a housing which has an introduction region having a predetermined volume and for introducing a liquid selected from the cell-containing solution containing two or more types of cells and a treating solution for treating the cells in the cell-containing solution into an interior, and a discharge flow path for discharging the liquid to the exterior; and a filter which is provided on a flow path between the introduction region and the discharge flow path and in which a plurality of through holes for the liquid to flow through is formed in the thickness direction, the method comprising:
 a step of draining a liquid to the cell trapping device for n (n is a natural number) times,   wherein from the first step of draining the liquid to the n-th step of draining the liquid, a liquid surface of the liquid in the introduction region on the filter is maintained at a predetermined liquid surface height, and when the n-th step of draining the liquid is completed, the discharging of the liquid from the cell trapping device is stopped in a state where the liquid surface is at a predetermined height in the filter.   
     
     
         2 . The cell trapping method according to  claim 1 ,
 wherein the housing includes an upper lid disposed above the introduction region and an introduction flow path of the liquid.   
     
     
         3 . The cell trapping method according to  claim 1 ,
 wherein before the first step of draining a liquid, a washing solution fills in the upstream side than the filter including the interior of the introduction region of the housing in advance.   
     
     
         4 . The cell trapping method according to  claim 1 ,
 wherein the step of draining a liquid includes at least one step of a step of draining a fixing solution, a step of draining a permeating solution, a step of draining a staining solution, and a step of draining a washing solution; and the step of draining a cell-containing solution.   
     
     
         5 . The cell trapping method according to  claim 1 ,
 wherein the n is 1 and the step of draining a liquid is the step of draining a cell-containing solution.   
     
     
         6 . The cell trapping method according to  claim 1 ,
 wherein in the step of draining a liquid, the liquid to be drained for the first time is a cell-containing solution, and the liquid to be drained for the second time is a washing solution.   
     
     
         7 . The cell trapping method according to  claim 1 ,
 wherein in the step of draining a liquid, at least part of the trapping target cell is immersed in the liquid in the introduction region on the filter.   
     
     
         8 . The cell trapping method according to  claim 1 ,
 wherein in the step of draining a liquid, the height of the liquid surface of the liquid in the introduction region on the filter is equal to or greater than the size of the trapping target cell.   
     
     
         9 . The cell trapping method according to  claim 1 ,
 wherein in the step of draining a liquid, the height of the liquid surface of the liquid in the introduction region on the filter is 50 μm or greater.   
     
     
         10 . The cell trapping method according to  claim 1 ,
 wherein a solvent of the cell-containing solution includes an aqueous solution containing whole blood, serum, or plasma of a mammal, or a protein originated therefrom.   
     
     
         11 . The cell trapping method according to  claim 1 ,
 wherein the filter is processed by using a polymeric material or a metal.   
     
     
         12 . The cell trapping method according to  claim 1 ,
 wherein in the step of draining a liquid, a rate of introducing the liquid to the cell trapping device is 50 to 1000 μL/min.   
     
     
         13 . A method for producing a specific cell-trapping device for producing a specific cell-trapping device which selectively traps a specific cell included in a cell-containing solution at a filter surface by filtering a liquid using a cell trapping device, the cell trapping device including a housing which has an introduction region having a predetermined volume and for introducing a liquid selected from the cell-containing solution containing two or more types of cells and a treating solution for treating the cells in the cell-containing solution into an interior, and a discharge flow path for discharging the liquid to the exterior; and a filter which is provided on a flow path between the introduction region and the discharge flow path and in which a plurality of through holes for the liquid to flow through is formed in the thickness direction, the method comprising:
 a step of draining a liquid to the cell trapping device for n (n is a natural number) times,   wherein from the first step of draining the liquid to the n-th step of draining the liquid, a liquid surface of the liquid in the introduction region on the filter is maintained at a predetermined liquid surface height, and when the n-th step of draining the liquid is completed, the discharging of the liquid from the cell trapping device is stopped in a state where the liquid surface is at a predetermined height in the filter.   
     
     
         14 - 24 . (canceled) 
     
     
         25 . A method for producing a specific cell-containing solution obtained by selectively trapping a specific cell included in a cell-containing solution at a filter surface by filtering a liquid using a cell trapping device, the cell trapping device including a housing which has an introduction region having a predetermined volume and for introducing a liquid selected from the cell-containing solution containing two or more types of cells and a treating solution for treating the cells in the cell-containing solution into an interior, and a discharge flow path for discharging the liquid to the exterior; and a filter which is provided on a flow path between the introduction region and the discharge flow path and in which a plurality of through holes for the liquid to flow through is formed in the thickness direction, the method comprising:
 a step of draining a liquid to the cell trapping device for n (n is a natural number) times,   wherein from the first step of draining the liquid to the n-th step of draining the liquid, a liquid surface of the liquid in the introduction region on the filter is maintained at a predetermined liquid surface height, and when the n-th step of draining the liquid is completed, the discharging of the liquid from the cell trapping device is stopped in a state where the liquid surface is at a predetermined height in the filter, and   wherein after the step of draining a liquid to the cell trapping device, a step of collecting a liquid existing in the housing is included.   
     
     
         26 - 39 . (canceled)