US2017269083A1PendingUtilityA1

Sample Collection and Analysis

59
Assignee: IDEXX LAB INCPriority: Mar 11, 2013Filed: May 31, 2017Published: Sep 21, 2017
Est. expiryMar 11, 2033(~6.5 yrs left)· nominal 20-yr term from priority
Inventors:Matthew Howard Myles
G01N 1/30G01N 2469/10Y10S436/811G01N 33/56983G01N 33/5695G01N 33/56911G01N 33/569
59
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Claims

Abstract

Management of the health status of an animal colony using a plurality of blood collection cards and the analysis of dried blood from members of the colony that has been collected on the cards. Members of the colony may be removed from the colony as a result of the analysis.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 . A method of determining a presence or absence of an infectious disease in a population of rodents, the method comprising:
 (a) receiving a plurality of absorbent blood collection materials from a user responsible for a population of rodents, wherein the blood collection materials have dried rodent blood;   (b) extracting the blood from the materials;   (c) conducting an immunoassay for analyzing the extracted blood for a presence or absence of at least one antibody for an infectious agent indicative of an infectious disease, thereby determining the presence or absence of the infectious disease in the rodent population; and   (d) reporting the results of the presence or absence of the infectious disease to the user.   
     
     
         2 . The method of  claim 1 , wherein the immunoassay comprises contacting the extracted blood with a fluorescently labeled binding partner for the antibody. 
     
     
         3 . The method of  claim 1 , wherein the immunoassay is a multiplex fluorescence immunoassay. 
     
     
         4 . The method of  claim 1 , where in the extracting comprises extracting the blood with a buffer comprising EDTA. 
     
     
         5 . The method of  claim 5 , wherein the immunoassay is a multiplex fluorescence immunoassay. 
     
     
         6 . The method of  claim 1 , wherein the members of the population of rodents are mice. 
     
     
         7 . The method of  claim 1 , wherein the members of the population of rodents are rats. 
     
     
         8 . The method of  claim 1 , wherein the material is associated with blood collection cards each have at least one collection area suitable for holding about 10-40 μL of whole blood. 
     
     
         9 . The method of  claim 8 , wherein the blood collection cards each have at least two collection areas. 
     
     
         10 . The method of  claim 9 , wherein the at least two collection areas are separated by a perforation that allows separation of the cards such that a single sample area is on each separated card. 
     
     
         11 . The method of  claim 6 , wherein the step of analyzing the extracted blood comprises analyzing for the presence or absence of seven or more diseases selected from the group consisting of:
 a. MHV;   b. MVN (MMV);   c. NS1 (Generic Parvovirus);   d. MPV (MPV1-5);   e. MNV;   f. TMEV;   g. EDIM;   h. Sendai virus;   i.  Mycoplasma pulmonis;      j. PVM;   k. REO3;   l. LCMV;   m. Ectromelia virus;   n. MAD1;   o. MAD2;   p. Polyoma virus;   q.  Encephalitozoon cuniculi;      r. CARB;   s.  Clostridium piliforme;      t. MCMV;   u. K virus;   v. Hantaan virus;   w. Lactate dehydrogenase-elevating virus; and   x. MTV.   
     
     
         12 . The method of  claim 7 , wherein the step of analyzing the extracted blood comprises analyzing for the presence or absence of seven or more diseases selected from the group consisting of:
 a. RCV;   b. NS1 (Generic Parvovirus);   c. RPV;   d. RMV;   e. KRV;   f. H-1;   g. RTV (Rat theilovirus);   h. Sendai virus;   i. PVM;   j.  Mycoplasma pulmonis;      k. REO3;   l. LCMV;   m. CARB;   n. Hantaan virus;   o.  Clostridim piliforme;      p. MAD1;   q. MAD2;   r.  Encephalitozoom cuniculi;  and   s. IDIR.   
     
     
         13 . The method of  claim 3 , wherein the members of the population of rodents are mice. 
     
     
         14 . The method of  claim 3 , wherein the blood on the materials was obtained from a facial vein. 
     
     
         15 . The method of  claim 3 , wherein the members of the population of rodents are rats. 
     
     
         16 . The method of  claim 3 , wherein the identity of the at least one of the rodent population and an individual member of the population is associated with each of the plurality of blood collection materials. 
     
     
         17 . The method of  claim 3 , wherein the material is associated with blood collection cards each have at least one collection area suitable for holding about 10-40 μL of whole blood. 
     
     
         18 . The method of  claim 17 , wherein the blood collection cards each have at least two collection areas. 
     
     
         19 . The method of  claim 18 , wherein the at least two collection areas are separated by a perforation that allows separation of the cards such that a single sample area is on each separated card. 
     
     
         20 . The method of  claim 3 , wherein the identity of at least one of the rodent population and an individual member of the population is associated with each blood collection material. 
     
     
         21 . The method of  claim 13 , wherein the step of analyzing the extracted blood comprises analyzing for the presence or absence of seven or more diseases selected from the group consisting of:
 a. MHV;   b. MVN (MMV);   c. NS1 (Generic Parvovirus);   d. MPV (MPV1-5);   e. MNV;   f. TMEV;   g. EDIM;   h. Sendai virus;   i.  Mycoplasma pulmonis;      j. PVM;   k. REO3;   l. LCMV;   m. Ectromelia virus;   n. MAD1;   o. MAD2;   p. Polyoma virus;   q.  Encephalitozoon cuniculi;      r. CARB;   s.  Clostridium piliforme;      t. MCMV;   u. K virus;   v. Hantaan virus;   w. Lactate dehydrogenase-elevating virus; and   x. MTV.   
     
     
         22 . The method of  claim 15 , wherein the step of analyzing the extracted blood comprises analyzing for the presence or absence of seven or more diseases selected from the group consisting of:
 a. RCV;   b. NS1 (Generic Parvovirus);   c. RPV;   d. RMV;   e. KRV;   f. H-1;   g. RTV (Rat theilovirus);   h. Sendai virus;   i. PVM;   j. Mycoplasma pulmonis;   k. REO3;   l. LCMV;   m. CARB;   n. Hantaan virus;   o.  Clostridim piliforme;      p. MAD1;   q. MAD2;   r.  Encephalitozoom cuniculi;  and   s. IDIR.   
     
     
         23 . The method of  claim 1 , wherein the dried rodent blood of the materials comprises rodent blood allowed to dry for about two hours.

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