Dna sample preparation and sequencing

Abstract

This disclosure describes, in one aspect, a method for preparing DNA molecule for sequencing. Generally, the method includes fragmenting the DNA molecule into double-stranded fragments; amplifying at least a portion of the double-stranded fragments; circularizing the fragments so that the first end of the fragment comprises a first loop connecting the strands and the second end of the fragment comprises a second loop connecting the strands; annealing a first sequencing primer to the first loop oriented to sequence at least a portion of one strand of the fragment; and annealing a second sequencing primer to the second loop oriented to sequence at least a portion of the other strand of the fragment. In another aspect, this disclosure describes a method for sequencing a DNA molecule. Generally, the method includes fragmenting the DNA molecule into double-stranded fragments; amplifying at least a portion of the double-stranded fragments; circularizing the fragments so that the first end of the fragment comprises a first loop connecting the strands and the second end of the fragment comprises a second loop connecting the strands; and sequencing at least one of the DNA strands.

1 . A method for preparing DNA molecule for sequencing, the method comprising:
 fragmenting the DNA molecule into double-stranded fragments;   amplifying at least a portion of the double-stranded fragments;   circularizing the fragments so that the first end of the fragment comprises a first loop connecting the strands and the second end of the fragment comprises a second loop connecting the strands;   annealing a first sequencing primer to the first loop oriented to sequence at least a portion of one strand of the fragment; and   annealing a second sequencing primer to the second loop oriented to sequence at least a portion of the other strand of the fragment.   
     
     
         2 - 17 . (canceled)