Method for producing cell culture vessel

Abstract

This invention provides a cost-effective technique for mass-production of a cell culture vessel that allows mass cell culture to be performed in a manner that allows cells to be stably detached from the culture vessel. Specifically, the invention provides a method for producing a cell culture vessel suitable for large-capacity culture comprising cutting a long-sized cell support film to obtain a sheet-like cell support film, fixing the film to a first member, which is not closed, and bonding other members thereto. Thus, a cell culture vessel comprising a container section in which a cell support film is fixed to an inner wall surface can be produced.

We claim: 
     
         1 . A cell support film provided with a half-cut long-sized detachable film comprising:
 a long-sized cell support film comprising a film substrate layer, and a stimuli-responsive polymer layer disposed on the film substrate layer,   a long-sized detachable film layer formed on a surface of the film substrate layer of the cell support film opposite from the stimuli-responsive polymer layer,   the cell support film comprising a plurality of regions surrounded with cut lines and separable as sheets, and   each cut line being a half-cut line reaching to a depth equivalent to the thickness of the cell support film, so that the cell support film is cut in the direction of film thickness, but the detachable film is not completely cut off.   
     
     
         2 . A sheet-like cell support film comprising:
 a film substrate layer and,   a stimuli-responsive polymer layer disposed on the film substrate layer,   wherein the sheet-like cell support film being in a shape such that it is able to cover approximately the entire area of the bottom inner surface of the container section of the flask-type cell culture vessel.