Thermus scotoductus nucleic acid polymerases

Abstract

The invention provides nucleic acids and polypeptides for a nucleic acid polymerase from a thermophilic organism, Thermus scotoductus . The invention also provides methods for using these nucleic acids and polypeptides.

1 . (canceled) 
     
     
         2 . (canceled) 
     
     
         3 . An isolated nucleic acid encoding a nucleic acid polymerase comprising any one of amino acid sequences SEQ ID NO:13-28. 
     
     
         4 . The isolated nucleic acid of  claim 3 , having a mutation that decreases 5-3′ exonuclease activity. 
     
     
         5 . (canceled) 
     
     
         6 . The isolated nucleic acid of  claim 3 , having a mutation that reduces discrimination against dideoxynucleotide triphosphates. 
     
     
         7 .- 35 . (canceled) 
     
     
         36 . An isolated nucleic acid polymerase comprising any one of amino acid sequences SEQ ID NO:13-28. 
     
     
         37 . The isolated nucleic acid polymerase of  claim 36 , having a mutation that decreases 5-3′ exonuclease activity. 
     
     
         38 . (canceled) 
     
     
         39 . The isolated nucleic acid polymerase of  claim 36 , having a mutation that reduces discrimination against dideoxynucleotide triphosphates. 
     
     
         40 .- 47 . (canceled) 
     
     
         48 . A method for thermocyclic amplification of nucleic acid comprising:
 (a) contacting a nucleic acid with a thermostable polypeptide having any one of SEQ ID NO: 13-28 under conditions suitable for amplification of said nucleic acid; and   (b) amplifying the nucleic acid.   
     
     
         49 . The method of  claim 48  wherein the thermocyclic amplification of the nucleic acid includes cycles of denaturation, primer annealing and primer extension. 
     
     
         50 . The method of  claim 48  wherein the thermocyclic amplification of the nucleic acid is performed by Strand Displacement Amplification. 
     
     
         51 . The method of  claim 48  wherein thermocyclic amplification of the nucleic acid is performed by Polymerase Chain Reaction. 
     
     
         52 . (canceled) 
     
     
         53 . The method of  claim 48  wherein the nucleic acid is DNA. 
     
     
         54 . The method of  claim 48  wherein the thermocyclic amplification is done to sequence the nucleic acid. 
     
     
         55 . (canceled)