Two part assembly

Abstract

A device that includes a first portion, the first portion including at least one fluid channel; a fluid actuator; and an introducer; a second portion, the second portion including at least one well, the well containing at least one material, wherein one of the first or second portion is moveable with respect to the other, wherein the introducer is configured to obtain at least a portion of the material from the at least one well and deliver it to the fluid channel, and wherein the fluid actuator is configured to move at least a portion of the material in the fluid channel.

1 . A device comprising:
 a first portion, the first portion comprising:
 at least one fluid channel; 
 a fluid actuator; and 
 an introducer; 
   a second portion, the second portion comprising:
 at least one well, the well containing at least one material, 
   wherein one of the first or second portion is moveable with respect to the other, wherein the introducer is configured to obtain at least a portion of the material from the at least one well and deliver it to the fluid channel, and wherein the fluid actuator is configured to move at least a portion of the material in the fluid channel.   
     
     
         2 . The device according to  claim 1 , wherein the second portion moves with respect to the first portion. 
     
     
         3 . (canceled) 
     
     
         4 . The device according to  claim 1 , wherein there are at least two wells with a first well comprising a buffer composition and a second well comprising a reagent composition. 
     
     
         5 . (canceled) 
     
     
         6 . (canceled) 
     
     
         7 . The device according to  claim 1 , wherein the fluid actuator comprises a port in fluid communication with the fluid channel. 
     
     
         8 . The device according to  claim 7 , wherein the port is configured to be in fluid communication with a pump that is external to the device. 
     
     
         9 . The device according to  claim 1 , wherein the first portion further comprises a sensor within the fluid channel. 
     
     
         10 . (canceled) 
     
     
         11 . The device according to  claim 1 , wherein the fluid channel is configured to be in fluid communication with a sample introduction pathway and the sample introduction pathway comprises a sample introduction chamber. 
     
     
         12 . (canceled) 
     
     
         13 . The device according to  claim 11 , wherein the sample introduction pathway is configured to obtain a sample from the sample introduction chamber and place it in fluid communication with the fluid channel. 
     
     
         14 . (canceled) 
     
     
         15 . The device according to  claim 11 , wherein the second portion comprises at least one empty well and the sample introduction pathway obtains the sample from the sample introduction chamber and places it in the empty well. 
     
     
         16 . The device according to  claim 1 , wherein the first portion comprises a plurality of wells and the introducer can randomly access any of the plurality of wells. 
     
     
         17 . (canceled) 
     
     
         18 . The device according to  claim 9 , wherein the sensor is a thin film bulk acoustic resonance (TFBAR) sensor. 
     
     
         19 . A system comprising:
 a device comprising:
 a first portion, the first portion comprising:
 at least one fluid channel; 
 a fluid actuator; 
 an introducer; and 
 a sensor; 
 
 a second portion, the second portion comprising:
 at least one well, the well containing at least one material, 
 wherein one of the first or second portion is moveable with respect to the other, wherein the introducer is configured to obtain at least a portion of the material from the at least one well and deliver it to the fluid channel, and wherein the fluid actuator is configured to move at least a portion of the material in the fluid channel; and 
 
   an external instrument,the external instrument configured to attain a signal from the sensor.   
     
     
         20 . The system according to  claim 19 , wherein the external instrument is further configured to be in fluid communication with the fluid actuator of the first portion of the sensor assembly. 
     
     
         21 . The system according to  claim 19 , wherein the external instrument comprises at least one pump. 
     
     
         22 . (canceled) 
     
     
         23 . (canceled) 
     
     
         24 . (canceled) 
     
     
         25 . A method comprising:
 providing a device comprising:
 a first portion, the first portion comprising:
 at least one fluid channel; 
 a fluid actuator; 
 an introducer; and 
 a sensor; and 
 
 a second portion, the second portion comprising:
 at least one well, the well containing at least one material, and 
 a sample well, 
 wherein one of the first or second portions is moveable with respect to the other wherein the introducer is configured to obtain at least a portion of the material from the at least one well and deliver it to the fluid channel, and wherein the fluid actuator is configured to move at least a portion of the material in the fluid channel; 
 
   placing a sample in the sample well;   obtaining at least a portion of the at least one material from the at least one well and depositing it in the fluidic pathway;   obtaining at least a portion of the sample from the sample well and depositing it in the fluidic pathway;   actuating fluid in the fluidic pathway so that at least a portion of the sample and the at least one material reach the sensor;   monitoring at least one signal from the sensor; and   depositing at least some of the sample, at least one material, or some combination thereof in the second portion of the device.   
     
     
         26 . The method according to  claim 25 , wherein the sample is placed in the sample well via the sample introduction pathway. 
     
     
         27 . The method according to  claim 26 , wherein the device further comprises a sample introduction chamber and the sample introduction pathway obtains the sample from the sample introduction chamber and deposits it in the sample well. 
     
     
         28 . (canceled) 
     
     
         29 . (canceled) 
     
     
         30 . The method according to claim,  25  further comprising mixing the at least one composition and the sample by placing at least a portion of the sample and the composition in a well of the second portion and further comprising obtaining the portion of the sample and the composition from the well and actuating it in the fluidic pathway, and repeating placing the portion of the sample and the material in the well at least two times in order to effectuate mixing. 
     
     
         31 . The method according to  claim 25 , wherein the step of actuating fluid in the fluidic pathway so that at least a portion of the mixed sample and the material reach the sensor comprises reversing the direction of flow at least once. 
     
     
         32 . (canceled) 
     
     
         33 . A device according to  claim 1 , wherein the fluid channel does not include valves. 
     
     
         34 .- 56 . (canceled)