Reagents, Kits And Methods, For Detecting Biological Molecules By Energy Transfer From An Activated Chemiluminescent Substrate To An Energy Acceptor Dye

Abstract

Reagents, kits and methods for detecting biological molecules by energy transfer from an activated chemiluminescent substrate to an energy acceptor dye such as a J-aggregated dye are described.

1 - 10 . (canceled) 
     
     
         11 . A kit for detecting analyte in a sample comprising:
 the article of claim  1 , wherein the one or more surface-bound biomolecular probes comprises a probe which is capable of binding to the analyte or, when the analyte is present, which binds to the analyte;   a chemiluminescent substrate; and   optionally, an enzyme labeled biomolecule or an enzyme labeled analyte.   
     
     
         12 . The kit of  claim 11 , wherein the chemiluminescent substrate is a dioxetane, an acridinium ester, an acridinium sulfonimide, an acridan, an acridan enolphosphate, luciferin or a luminol. 
     
     
         13 . The kit of  claim 12 , wherein the chemiluminescent substrate is a 1,2-dioxetane substrate. 
     
     
         14 . The kit of  claim 13 , wherein the chemiluminescent substrate has a structure as set forth below: 
       
         
           
           
               
               
           
         
       
     
     
         15 - 16 . (canceled) 
     
     
         17 . The kit of  claim 11 , wherein the surface-bound biomolecular probe is an antibody and the analyte is an antigen for the antibody and wherein the kit comprises an enzyme-labeled antibody which is capable of binding to the analyte when the analyte is bound to the surface-bound biomolecular probe. 
     
     
         18 . The kit of  claim 14 , wherein the kit comprises the enzyme-labeled biomolecule or the enzyme-labeled analyte and wherein the enzyme label is alkaline-phosphatase. 
     
     
         19 - 67 . (canceled)