Isolated novel nucleic acid and protein molecules from soy and methods of using those molecules to generate transgenic plants with enhanced agronomic traits

Abstract

This disclosure provides purified nucleic acids and polypeptides. Also provided are transgenic plants, seeds, and plant cells containing DNA for expression of the proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants, methods of making such plants and methods of making agricultural commodity including seeds and hybrid seeds from such plants.

1 . (canceled) 
     
     
         2 . (canceled) 
     
     
         3 . A recombinant DNA construct comprising a promoter that is functional in a plant cell and that is operably linked to a polynucleotide that:
 (a) encodes a polypeptide having an amino acid sequence having at least 95% identity over at least 95% of the length of a reference sequence selected from the group consisting of SEQ ID NO: 116779-233556; or   (b) encodes an RNA molecule capable of binding to an RNA encoding a polypeptide that is at least 95% identical over at least 95% of the length of a reference sequence selected from the group consisting of SEQ ID NO: 116779-233556; and   
       wherein said RNA molecule suppresses the expression of said polypeptide. 
     
     
         4 . A transgenic plant cell comprising the recombinant DNA construct of  claim 3 . 
     
     
         5 . (canceled) 
     
     
         6 . (canceled) 
     
     
         7 . A transgenic plant or seed comprising a plurality of plant cells of  claim 4 . 
     
     
         8 . The transgenic plant or seed of  claim 7 , which is homozygous for said recombinant DNA. 
     
     
         9 . (canceled) 
     
     
         10 . The transgenic plant or seed of  claim 7 , wherein said transgenic plant or seed is from a corn, soybean, cotton, canola, alfalfa, wheat, rice, sugarcane, or sugar beet plant. 
     
     
         11 . (canceled) 
     
     
         12 . A method for manufacturing non-natural, transgenic seed comprising a stably-integrated, recombinant DNA construct of  claim 3 , said method comprising:
 (a) screening a population of plants for said recombinant DNA construct;   (b) selecting from said population one or more plants that comprise said recombinant DNA construct, and   (c) collecting seed from selected plant from step b.   
     
     
         13 . The method of  claim 12 , wherein said method for manufacturing said transgenic seed further comprises:
 (a) verifying that said recombinant DNA is stably integrated in said selected plants, and   (b) analyzing tissue of said selected plant to determine the expression or suppression of a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:116779-233556.   
     
     
         14 . The method of  claim 13 , wherein said seed is corn, soybean, cotton, canola, alfalfa, wheat, rice, sugarcane, or sugar beet seed. 
     
     
         15 . A method of producing hybrid corn seed comprising:
 (a) acquiring hybrid corn seed from an herbicide tolerant corn plant which also has a stably-integrated, recombinant DNA construct of  claim 3 ;   (b) producing corn plants from said hybrid corn seed, wherein a fraction of the plants produced from said hybrid corn seed is homozygous for said recombinant DNA construct, a fraction of the plants produced from said hybrid corn seed is hemizygous for said recombinant DNA construct, and a fraction of the plants produced from said hybrid corn seed has none of said recombinant DNA construct;   (c) selecting corn plants which are homozygous and hemizygous for said recombinant DNA construct by treating with an herbicide;   (d) collecting seed from herbicide-treated-surviving corn plants and planting said seed to produce further progeny corn plants;   (e) repeating steps (c) and (d) at least once to produce an inbred corn line; and   (f) crossing said inbred corn line with a second corn line to produce hybrid seed.   
     
     
         16 . (canceled) 
     
     
         17 . The recombinant DNA construct of  claim 3 , wherein said RNA molecule is a microRNA molecule. 
     
     
         18 . The transgenic plant or seed of  claim 7 , further comprising DNA expressing a protein that provides tolerance from exposure to an herbicide comprising an agent applied at levels that are lethal to a wild type of said plant cell nucleus. 
     
     
         19 . The transgenic plant or seed of  claim 18 , wherein said agent is a glyphosate, dicamba, glufosinate, sulfonylurea, bromoxynil, or norflurazon compound. 
     
     
         20 . The transgenic plant or seed of  claim 7 , wherein said recombinant DNA construct encodes a double-stranded RNA, an antisense RNA, or a microRNA. 
     
     
         21 . The transgenic plant or seed of  claim 20 , wherein said microRNA has at least 95% identity to a sequence, or complement thereof, of a fragment of at least 20, 21, or 22 consecutive nucleotides of a reference sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 116778. 
     
     
         22 . The transgenic plant or seed of  claim 20 , wherein said microRNA suppresses the expression of an endogenous polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 116779-233556. 
     
     
         23 . The transgenic plant or seed of  claim 7 , wherein said RNA molecule suppresses the expression of an endogenous polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 116779-233556.