Lipid particles with cationic lipids for rna delivery

Abstract

What is described is a lipid particle comprising the compound of formula I wherein R 1 consists of alkyl, alkenyl, alkynyl, or cholesteryl; R 2 consists of alkyl or alkenyl; L 1 consists of alkyl, alkylene or alkenyl; X 1 consists of —O—(CO)— or —(CO)—O; X 2 consists of S or O; L 2 consists of a bond or alkylene; R 3 consists of alkylene; and R 4 and R 5 are the same or different, each consisting of alkyl; or a pharmaceutical preparation thereof.

What is claimed: 
     
         1 . Lipid particles comprising a cationic lipid, wherein the cationic lipid comprises a compound of formula I 
       
         
           
           
               
               
           
         
       
       wherein
 R 1  consists of a linear or branched alkyl consisting of 1 to 20 carbons, an alkenyl or alkynyl consisting of 2 to 12 carbons, or a cholesteryl; 
 R 2  consists of a linear or branched alkyl consisting of 1 to 20 carbons or an alkenyl consisting of 2 to 20 carbons; 
 L 1  consists of a linear alkyl consisting of 3 to 9 carbons or, when R 1  consists of a cholesteryl then L 1  consists of a linear alkylene or alkenyl consisting of 3 to 4 carbons; 
 X 1  consists of —O—(CO)— or —(CO)—O—; 
 X 2  consists of S or O; 
 L 2  consists of a bond or a linear alkylene of 1 to 6 carbons; 
 R 3  consists of a linear or branched alkylene with 1 to 6 carbons; and 
 R 4  and R 5  are the same or different, each consisting of a linear or branched alkyl of 1 to 6 carbons; 
 
       or a pharmaceutical preparation thereof.