Antibody specific for trans-resveratrol and use thereof

Abstract

The present invention provides a method of detecting or measuring trans-Resveratrol (tRV) in a sample, comprising: contacting a sample to be tested with a antibody against tRV, or an antigen binding fragment of such an antibody; and detecting or measuring any tRV bound by the antibody or antibody fragment. The invention also provides an antibody against trans-Resveratrol.

1 . A method of detecting or measuring trans-Resveratrol (tRV) in a sample, comprising:
 (i) contacting a sample to be tested with a monoclonal antibody against tRV, or an antigen binding fragment of such an antibody; and   (ii) detecting or measuring any tRV bound by the antibody or antibody fragment.   
     
     
         2 . The method of  claim 1 , where:
 (a) the antibody or antibody fragment of (i) is bound to a support; and/or   (b) the binding of tRV to the antibody or fragment of (i) is detected with a second antibody against tRV or antigen binding fragment of such an antibody.   
     
     
         3 . The method of  claim 1 , where the method is an ELISA method or a method performed using an antibody based chip or sensor. 
     
     
         4 . The method of  claim 1 , wherein the sample to be tested is an agricultural, pharmaceutical, nutraceutical or cosmetic sample or is a biological sample from a human. 
     
     
         5 . The method of  claim 4 , wherein the biological sample is human serum. 
     
     
         6 . The method of  claim 1 , where the antibody or antibody fragment of (i) is that obtainable from the hybridoma deposited under Accession Number VKPM H-121 or the hybridoma deposited under VKPM H-122. 
     
     
         7 . The method of  claim 1 , wherein the method provides a measurement for trans-Resveratrol defined by reference to a standard scale, chart, score for trans-Resveratrol 
     
     
         8 . An antibody against tRV, or an antigen binding fragment of such an antibody, where the antibody or antibody fragment are as defined  claim 1 . 
     
     
         9 . A hybridoma selected from the hybridoma deposited under Accession Number VKPM H-121, the hybridoma deposited under Accession Number VKPM H-122 and a hybridoma producing the same antibody as those deposited under Accession Numbers VKPM H-121 and VKPM H-122. 
     
     
         10 . A kit for detecting or measuring trans-Resveratrol (tRV), where the kit comprises the antibody or antibody fragment of  claim 8 . 
     
     
         11 . The kit of  claim 10 , wherein the kit also comprises:
 (a) a support for immobilising the antibody to;   (b) a standard for tRV   (c) means for detecting binding of tRV to the antibody or fragment thereof;   and/or instructions for performing the assay.