EHD2 Antibody and Application Thereof in Preparation of Immunohistochemical Detection Reagent for Breast Cancer

Abstract

An EHD2 antibody and an application thereof in the preparation of an immunohistochemical detection reagent for breast cancer. The antibody can be used for specifically recognizing a human EHD2 protein, and the amino acid sequence on a recognition site is: 503-SEQ ID NO:1-543.

1 . An antibody specific to a human EHD2 protein, characterized in that the antibody is able to specifically recognize the human EHD2 protein, and an amino acid sequence on a recognition site is: 503-SEQ ID NO: 1-543. 
     
     
         2 . A method of diagnosing or prognosing breast cancer by an immunohistochemical method, the method comprising the use of the EHD2 gene and its encoded protein, wherein an internationally universal sequence number for the EHD2 gene in GeneBank is: NM_014601, characterized in that an internationally universal sequence number for the protein coded by the EHD2 gene in GeneBank is: NP_055416. 
     
     
         3 . A method of diagnosing or prognosing breast cancer, the method comprising the use of the antibody of  claim 1 . 
     
     
         4 . A method for diagnosing or prognosing breast cancer by an immunohistochemical method, the method comprising the use of the EHD2 gene and its encoded protein, wherein an internationally universal sequence number for the EHD2 gene in GeneBank is: NM_014601. 
     
     
         5 . A method of diagnosing or prognosing breast cancer comprising using the antibody of  claim 1 . 
     
     
         6 . A polypeptide, characterized in that an amino acid sequence for the polypeptide is SEQ ID NO: 1. 
     
     
         7 . The polypeptide according to  claim 6 , characterized in that the polypeptide undergoes a modification with its amino acid sequence as the core sequence. 
     
     
         8 . The polypeptide according to  claim 7 , characterized in that the modification is achieved by connecting a cysteine to an N-terminal of the polypeptide. 
     
     
         9 . A method of preparing the antibody of  claim 1 , the method comprising using a polypeptide having the amino acid sequence as set forth in SEQ ID NO: 1. 
     
     
         10 . A method according to  claim 9 , further comprising purifying protein antigens by the antibody. 
     
     
         11 . A method of preparing an antibody specific to an EHD2 protein, the method comprising using the polypeptide of  claim 6 . 
     
     
         12 . A method according to  claim 11  further comprising purifying the antibody using a polypeptide, wherein an amino acid sequence for the polypeptide is SEQ ID NO: 1. 
     
     
         13 . An immunohistochemical reagent for diagnosis or prognosis judgment of breast cancer comprising the antibody of  claim 1  as a primary antibody or a core antibody. 
     
     
         14 . A method of preparing the antibody of  claim 1 , the method comprising using a polypeptide having an amino acid sequence as set forth in SEQ ID NO: 1, wherein the polypeptide undergoes a modification with its amino acid sequence as the core sequence. 
     
     
         15 . A method of preparing the antibody of  claim 1 , the method comprising using a polypeptide having an amino acid sequence as set forth in SEQ ID NO: 1, wherein the polypeptide undergoes a modification with its amino acid sequence as the core sequence and wherein the modification is achieved by connecting a cysteine to an N-terminal of the polypeptide. 
     
     
         16 . A method of preparing an antibody specific to an EHD2 protein, the method comprising using a polypeptide having an amino acid sequence as set forth in SEQ ID NO: 1, wherein the polypeptide undergoes a modification with its amino acid sequence as the core sequence. 
     
     
         17 . A method of preparing an antibody specific to an EHD2 protein, the method comprising using a polypeptide having an amino acid sequence as set forth in SEQ ID NO: 1, wherein the polypeptide undergoes a modification with its amino acid sequence as the core sequence and wherein the modification is achieved by connecting a cysteine to an N-terminal of the polypeptide. 
     
     
         18 . A method of preparing an antibody specific to an EHD2 protein, the method comprising using the polypeptide of  claim 7 . 
     
     
         19 . A method of preparing an antibody specific to an EHD2 protein, the method comprising using the polypeptide of  claim 8 . 
     
     
         20 . A method of preparing an antibody specific to an EHD2 protein, the method comprising:
 using a polypeptide, wherein:
 an amino acid sequence for the polypeptide is SEQ ID NO: 1, and 
 the polypeptide is the polypeptide of  claim 7 ; and 
   purifying the antibody using the polypeptide.   
     
     
         21 . A method of preparing an antibody specific to an EHD2 protein, the method comprising:
 using a polypeptide, wherein:
 an amino acid sequence for the polypeptide is SEQ ID NO: 1, and 
 the polypeptide is the polypeptide of  claim 8 ; and 
   purifying the antibody using the polypeptide.