Microscope

Abstract

A microscope including a sample carrier configured to support a sample. Excitation light illuminates the sample via an excitation beam path. Detection light from the sample is guided to detection means via a detection beam path. Through an objective arranged along the optical axis, excitation light is guided in direction of the sample carrier and detection light coming from the sample is guided in direction of the detection means. Beam-splitting means separate excitation light and detection light. Also provided are means for generating a light sheet from excitation light, and means for illuminating the sample with this light sheet. The light sheet lies in a plane at a nonzero angle to the optical axis. The means for illuminating the sample include an optical-deflecting device arranged on or at the sample carrier, which deflects excitation light from the objective into the plane of the light sheet via an optically active surface.

1 . An arrangement for a microscope, the arrangement comprising:
 a sample carrier configured to hold a sample; and   an optical deflecting device that is arranged on or at the sample carrier, the deflecting device having at least one optically active surface configured to deflect excitation light.   
     
     
         2 . The arrangement according to  claim 1 ;
 wherein the optical deflecting device is configured as a mirror element with at least one optically active surface formed as a mirror surface.   
     
     
         3 . The arrangement according to  claim 1 ;
 wherein the optical deflecting device is configured as a prism element with an optically active surface that is formed as an inner side of a prism.   
     
     
         4 . The arrangement according to  claim 1 ;
 wherein the at least one optically active surface is comprised of a plurality of microprisms.   
     
     
         5 . The arrangement according to  claim 1 ;
 wherein the optically active surface is formed in a funnel-shaped manner so as to at least partially surround the sample.   
     
     
         6 . The arrangement according to  claim 1 :
 wherein the sample carrier comprises an opening configured to receive the optical deflecting device.   
     
     
         7 . The arrangement according to  claim 1 ;
 wherein the optical deflecting device comprises a structure configured to be placed on the sample carrier, or on a sample stage of a microscope, so as to surround the sample with a funnel-shaped optically active surface.   
     
     
         8 . The arrangement according to  claim 1 ;
 wherein the optical deflecting device is detachably outfitted with a protective device configured to protect the deflecting device when culturing and/or transporting.   
     
     
         9 . The arrangement according to  claim 1 ;
 wherein the sample carrier comprises at least one well with at least one lateral wall; and   wherein the at least one lateral wall corresponds to the optically active surface.   
     
     
         10 . The arrangement according to  claim 2 ;
 wherein the at least one mirror surface forms an angle of 45° with a plane of the sample carrier.   
     
     
         11 . The arrangement according to  claim 3 ;
 wherein the optically active surface forms an angle of 45° with a plane of the sample carrier.   
     
     
         12 . The arrangement according to  claim 4 ;
 wherein the optically active surface on average forms an angle of 45° with a plane of the sample carrier.