Enzyme-immobilized porous membrane and preparation method of antibiotics using the same

Abstract

The present disclosure relates to an enzyme-immobilized porous membrane and a preparation method of antibiotics using the same, and more specifically, to an enzyme-immobilized porous membrane prepared by immobilizing a specific enzyme through dead-end filtration, and a preparation method of antibiotics with a high yield using the enzyme-immobilized porous membrane. According to various exemplary embodiments of the present disclosure, the enzyme capable of promoting the synthesis reaction of the antibiotic substance is able to be stably immobilized in the porous membrane by passing the solution of enzyme through the membrane. In addition, it is possible to provide antibiotics with a high yield by preparing the antibiotics by passing the reactant solution through the enzyme-immobilized porous membrane.

1 . An enzyme-immobilized porous membrane in which an enzyme promoting a synthesis reaction of an antibiotic substance is immobilized,
 wherein the porous membrane is three-dimensionally interconnected by pores,   the porous membrane forms a three-dimensional network by polymerizing a first monomer and a second monomer each having two to four functional groups,   the functional group of the first monomer is an amino group,   the functional group of the second monomer is an isocyanate group, an acyl halide group or an ester group,   the first monomer and/or the second monomer has four functional groups, and   the enzyme is at least one selected from the group consisting of penicillin G acylase, penicillin V acylase, and cephalosporin C acylase.   
     
     
         2 . The enzyme-immobilized porous membrane according to  claim 1 , wherein the antibiotic substance is a penicillin-based substance, or a cephalosporin-based substance. 
     
     
         3 . A preparation method of antibiotics comprising:
 (B) permeating a derivative solution of an antibiotic substance through the enzyme-immobilized porous membrane according to  claim 1 .   
     
     
         4 . The preparation method according to  claim 3 , wherein the derivative of the antibiotic substance includes a first derivative and a second derivative,
 the first derivative and the second derivative are mixed at a molar (M) ratio of 1:1 to 3,   the first derivative and the second derivative are different from each other, and are each independently at least one selected from the group consisting of 6-aminopenicillanic acid, p-hydroxyphenylglycine methyl ester, 7-aminodesacetoxycephalosporanic acid, and phenylglycine.   
     
     
         5 . The preparation method according to  claim 4 , wherein the first derivative is the 6-aminopenicillanic acid, and the second derivative is the p-hydroxyphenylglycine methyl ester. 
     
     
         6 . The preparation method according to  claim 3 , further comprising:
 (A) preparing the derivative solution of the antibiotic substance, wherein step (A) includes:   (a-1) preparing a first derivative solution;   (a-2) preparing a second derivative solution; and   (a-3) mixing the first derivative solution with the second derivative solution.   
     
     
         7 . The preparation method according to  claim 3 , wherein the permeating of step (B) is performed by dead-end filtration. 
     
     
         8 . The preparation method according to  claim 3 , wherein step (B) is performed at a pressure of 2 to 7 bar in a nitrogen atmosphere. 
     
     
         9 . The preparation method according to  claim 3 , wherein the antibiotic substance is a penicillin-based substance, or a cephalosporin-based substance. 
     
     
         10 . A preparation method of antibiotics comprising:
 (B) permeating a derivative solution of an antibiotic substance through the enzyme-immobilized porous membrane according to  claim 2 .