Inclusion compound of 3',5'-cyclicdiadenylic acid, and method for producing same

Abstract

Though 3′,5′-cyclic diadenylic acid was conventionally provided only as a freeze-dried product, a solid material other than a freeze-dried product and a manufacturing method thereof is provided. By a step of adding acid to an aqueous solution of 3′,5′-cyclic diadenylic acid so as to lower pH to 1 to 3, an inclusion compound of 3′,5′-cyclic diadenylic acid can be obtained. Said manufacturing method is an extremely simple and easy method and does not need a special machine or the like.

1 . A manufacturing method of an inclusion compound of 3′,5′-cyclic diadenylic acid comprising a step of adding acid to an aqueous solution of 3′,5′-cyclic diadenylic acid so as to lower pH to 1 to 3. 
     
     
         2 . A manufacturing method of an inclusion compound of 3′,5′-cyclic diadenylic acid comprising the following steps (1) to (3):
 (1) a step of adding acid to an aqueous solution of 3′,5′-cyclic diadenylic acid so as to lower pH to 1 to 3, 
 (2) a step of heating the aqueous solution of 3′,5′-cyclic diadenylic acid to 50 to 70° C., and 
 (3) a step of cooling the aqueous solution of 3′,5′-cyclic diadenylic acid until it reaches 1 to 10° C. 
 
     
     
         3 . An inclusion compound of 3′,5′-cyclic diadenylic acid, wherein 1 to 7 molecules of water attach or bond to one molecule of 3′,5′-cyclic diadenylic acid. 
     
     
         4 . An inclusion compound of 3′,5′-cyclic diadenylic acid of  claim 3 , having purity of 97% or more as measured by high-performance liquid chromatography. 
     
     
         5 . An inclusion compound of 3′,5′-cyclic diadenylic acid of  claim 3 , having purity of 99% or more as measured by high-performance liquid chromatography. 
     
     
         6 . An inclusion compound of 3′,5′-cyclic diadenylic acid of  claim 3 , wherein the inclusion compound is in the form of a crystal. 
     
     
         7 . An inclusion compound of 3′,5′-cyclic diadenylic acid of  claim 6 , wherein the inclusion compound shows characteristic peaks around 9.2, 15.2, 19.0, 20.6 and 26.8(°) in diffraction angle (2θ) in X-ray powder analysis.