Bioactive compositions and methods for their preparation and use

Abstract

A method for preparing a bioactive composition containing conditioned cell culture medium is disclosed. The method comprises culturing cells of two or more eukaryotic cell line to form conditioned culture media, separating the cultured cells from the conditioned culture media, and combining conditioned culture media to form a bioactive composition. Novel bioactive compositions, formulations and their use in treating of a variety of diseases and health conditions are also disclosed.

1 - 16 . (canceled) 
     
     
         17 . A composition comprising extracellular products secreted by cells of (1) an adult human adipose derived stem cell (ADSC) line, (2) a fetal human fibroblast cell line (HDF-f), and (3) a hair dermal papilla (HDP) cell line. 
     
     
         18 . The composition of  claim 17 , wherein said ADSC line is a StemPro® human adipose-derived stem cell (hADSC) line provided by Life Technologies Catalog Number R7788115. 
     
     
         19 . The composition of  claim 17 , wherein said fibroblast cell line is a fetal human dermal fibroblast (HDF-f) cell line provided by ScienCell Research Laboratories Catalog Number 2300. 
     
     
         20 . The composition of  claim 17 , wherein said HDP cell line is a
 human hair dermal papilla cell (HHDPC) line provided by ScienCell Research Laboratories Catalog Number 2400.   
     
     
         21 . The composition of  claim 17 , wherein
 said ADSC line is a StemPro® human adipose-derived stem cell(hADSC) line provided by Life Technologies Catalog Number R7788115,   said fibroblast cell line is a fetal human dermal fibroblast (HDF-f) cell line provided by ScienCell Research Laboratories Catalog Number 2300, and   said HDP cell line is a human hair dermal papilla cell (HHDPC) line provided by ScienCell Research Laboratories Catalog Number 2400.   
     
     
         22 . The composition of  claim 17 , wherein said extracellular products secreted by cells of said adult human adipose derived stem cell (ADSC) line, said fetal human fibroblast cell line, and said hair dermal papilla (HDP) cell line are combined in a ratio of about 33/33/33 by volume. 
     
     
         23 . The composition of  claim 17 , wherein said composition is further formulated for topical or transdermal administration. 
     
     
         24 . A method for treating or a hair condition in a subject in need thereof comprising administering to said subject a therapeutically effective amount of the composition of  claim 17 . 
     
     
         25 . A method for treating a hair condition in a subject in need thereof comprising administering to said subject a therapeutically effective amount of a composition prepared by
 culturing cells of a first cell line, a second cell line, and a third cell line in one or more culture media, wherein said cultured cells secrete extracellular products into said culture media so that one or more conditioned culture media are formed;   separating said one or more conditioned culture media from respective said cultured cells; and   combining said one or more conditioned culture media to form the composition,   wherein said first, second, and third cell lines are an adult human adipose derived stem cell (ADSC) line, a fetal human fibroblast cell line, and a hair dermal papilla (HDP) cell line, respectively.   
     
     
         26 - 30 . (canceled) 
     
     
         31 . The method of  claim 24 , comprising administering to said subject a therapeutically effective amount of said composition, thereby stimulating hair growth. 
     
     
         32 . The method of  claim 31 , wherein said hair condition to be treated is selected from the group consisting of pattern baldness, alopecia caused by chemotherapy, hair thinning due to aging, illness, stress, traction alopecia, and genetic disorders. 
     
     
         33 . The method of  claim 31 , wherein said subject exhibits an improved stimulation of hair growth in the scalp, back, beard, eyebrows, lashes, leg, arm, pubic region, or a combination of any thereof. 
     
     
         34 . The method of  claim 31 , wherein said hair growth is due to thickened hair sheath diameter, increased hair diameter, increased rate of growth in hair length, increased thickness, differentiation of quiescent hair follicles into more mature forms, the appearance of proliferation of new hair follicle, or a combination of any thereof. 
     
     
         35 . The method of  claim 31 , wherein said composition is administered topically. 
     
     
         36 . The method of  claim 31 , wherein said composition is administered transdermally. 
     
     
         37 . The method of  claim 31 , wherein said composition is administered in a form selected from the group consisting of a lotion, a gel, an ointment, a cream, a powder, a salve, a foam, a spray, an exosome, and a liposome. 
     
     
         38 . The method of  claim 24 , wherein said extracellular products are extracellular products secreted by cells of said ADSC, HDF-f, and HDP cell lines which have been cultured for at least 2 days, at least 5 days, at least 7 days, at least 8 days, or at least 10 days. 
     
     
         39 . The method of  claim 24 , wherein said extracellular products are extracellular products secreted by cells of said ADSC, HDF-f, and HIP cell lines which have been cultured until said cell cultures reache at least 85%, at least 90%, at least 95%, or at least 98% cell confluence. 
     
     
         40 . The method of  claim 24 , wherein said extracellular products are extracellular products secreted by cell cultures of said ADSC, HDF-f, and HDP cell lines and wherein of at least one of said cell cultures have been passaged multiple times to produce said extracellular products. 
     
     
         41 . The method of  claim 40 , wherein said cell cultures have been passaged at least 2, 3, 4, 5, 6, 7, 8, 9, or 10 times to produce said extracellular products. 
     
     
         42 . The method of  claim 40 , wherein cells of at least one of said cell lines are passaged after reaching at least 85%, at least 90%, at least 95%, or at least 98% cell confluence. 
     
     
         43 . The method of  claim 24 , wherein said cells of the first, second, and third cell lines are cultured individually to form respective first, second, and third extracellular products. 
     
     
         44 . The method of  claim 43 , wherein said first, second, and third extracellular products are combined in a ratio of about 33/33/33 by volume.