Silicon-Vacancy-Doped Nanodiamonds For Molecular And Cellular Imaging

Abstract

An imaging agent for detecting analytes in a biological environment includes functionalized, silicon vacancy center-containing nanodiamonds. Individual nanodiamonds of the imaging agent include at least one silicon vacancy center. The at least one silicon vacancy center can emit light having a wavelength in a narrow band in response to illumination having any wavelength in a wide range of wavelengths. The nanodiamonds are functionalized to selectively interact with an analyte of interest. The nanodiamonds can additionally include other color centers, and the imaging agent can include a plurality of sets of nanodiamonds having detectably unique ratios of silicon vacancy centers to other color centers. The silicon vacancy centers in the nanodiamonds can have a preferred orientation enabling orientation tracking of individual nanodiamonds or other applications. A method for detecting properties of the analyte of interest by interacting with the imaging agent is also provided.

1 - 20 . (canceled) 
     
     
         21 . A method comprising:
 exposing an environment to illumination from a light source, wherein the illumination comprises excitation light having a wavelength between 1050 and 1200 nanometers, wherein the environment comprises silicon-vacancy nanodiamonds, wherein each of the silicon-vacancy nanodiamonds has at least one silicon vacancy center and is functionalized to selectively interact with an analyte in the environment, and wherein the excitation light is absorbed by the silicon vacancy centers through two-photon absorption and causes the silicon vacancy centers to emit light in a band of wavelengths centered at approximately 738 nanometers and having a full width at half-maximum of less than 15 nanometers; and   detecting, by a light sensor, one or more properties of the light emitted by the silicon vacancy centers in response to the excitation light.   
     
     
         22 . The method of  claim 21 , wherein the environment is a biological environment. 
     
     
         23 . The method of  claim 22 , wherein the biological environment is a portion of subsurface vasculature. 
     
     
         24 . The method of  claim 23 , wherein the analyte is a cell. 
     
     
         25 . The method of  claim 21 , further comprising introducing the silicon-vacancy nanodiamonds into the environment. 
     
     
         26 . The method of  claim 21 , further comprising:
 determining one or more properties of the silicon-vacancy nanodiamonds in the environment based on the detected one or more properties of the light emitted by the silicon vacancy centers; and   determining a property of the analyte based on the determined one or more properties of the silicon-vacancy nanodiamonds.   
     
     
         27 . The method of  claim 21 , wherein the silicon vacancy centers have a preferred orientation, wherein detecting one or more properties of the light emitted by the silicon vacancy centers comprises detecting a polarization of the emitted light, further comprising:
 detecting binding of a silicon-vacancy nanodiamond to the analyte based on at least the detected one or more properties of the emitted light.   
     
     
         28 . The method of  claim 21 , wherein the silicon vacancy centers have a preferred orientation, wherein the illumination has a specified polarization, further comprising:
 detecting binding of a silicon-vacancy nanodiamond to the analyte based on at least the specified polarization and the detected one or more properties of the emitted light.   
     
     
         29 . The method of  claim 21 , wherein the environment further comprises nitrogen-vacancy nanodiamonds, and wherein each of the nitrogen-vacancy nanodiamonds has at least one nitrogen vacancy center and is functionalized to selectively interact with a second analyte in the environment, further comprising:
 exposing the environment to additional illumination, and wherein the additional illumination causes the nitrogen vacancy centers to emit light;   detecting one or more properties of the light emitted by the nitrogen vacancy centers in response to the additional illumination; and   determining whether a nanodiamond in the environment was a silicon-vacancy nanodiamond or a nitrogen-vacancy nanodiamond based on at least the detected one or more properties of the light emitted by the silicon vacancy centers and the detected one or more properties of the light emitted by the nitrogen vacancy centers.   
     
     
         30 . The method of  claim 29 , wherein each of the silicon-vacancy nanodiamonds has at least one nitrogen vacancy center, wherein the ratio of the concentration of silicon vacancy centers to the concentration of nitrogen vacancy centers in the silicon-vacancy nanodiamonds is a first ratio, wherein each of the nitrogen-vacancy nanodiamonds has at least one silicon vacancy center, wherein the ratio of the concentration of silicon vacancy centers to the concentration of nitrogen vacancy centers in the nitrogen-vacancy nanodiamonds is a second ratio, wherein the first and second ratios are different. 
     
     
         31 . The method of  claim 21 , wherein the light source is in a wearable device. 
     
     
         32 . The method of  claim 21 , wherein the light sensor is in a wearable device. 
     
     
         33 . A device comprising:
 a light source that can direct excitation light having a wavelength between 1050 and 1200 nanometers to an environment comprising silicon-vacancy nanodiamonds, wherein each silicon-vacancy nanodiamond has at least one silicon vacancy center and is functionalized to selectively interact with an analyte in the environment, and wherein the excitation light is absorbed by the silicon vacancy centers through two-photon absorption and causes the silicon vacancy centers to emit light in a band of wavelengths centered at approximately 738 nanometers and having a full width at half-maximum of less than 15 nanometers; and   a light sensor that can detect one or more properties of the light emitted by the silicon vacancy centers in response to the excitation light.   
     
     
         34 . The device of  claim 33 , wherein the environment is a biological environment. 
     
     
         35 . The device of  claim 34 , wherein the biological environment is a portion of subsurface vasculature. 
     
     
         36 . The device of  claim 35 , further comprising:
 a housing, wherein the light source and light sensor are disposed in the housing; and   a mount for mounting the housing to an external body surface proximate the portion of subsurface vasculature such that the light source can illuminate the silicon-vacancy nanodiamonds in the portion of subsurface vasculature and the light sensor can detect the one or more properties of the light emitted by the silicon vacancy centers.   
     
     
         37 . The device of  claim 33 , further comprising:
 a processor;   a computer readable medium storing program instructions, wherein the program instructions are executable by the processor to perform functions comprising:
 operating the light source to illuminate the environment; 
 operating the light sensor to detect the one or more properties of light emitted by the silicon vacancy centers; 
 determining one or more properties of the silicon-vacancy nanodiamonds in the environment based on the detected one or more properties of the emitted light; and 
 determining a property of the analyte based on the determined one or more properties of the silicon-vacancy nanodiamonds. 
   
     
     
         38 . The device of  claim 37 , wherein the silicon vacancy centers have a preferred orientation, wherein operating the light sensor to detect the one or more properties of light emitted by the silicon vacancy centers comprises operating the light sensor to detect a polarization of the emitted light. 
     
     
         39 . The device of  claim 37 , wherein the environment further comprises nitrogen-vacancy nanodiamonds, and wherein each of the nitrogen-vacancy nanodiamonds has at least one nitrogen vacancy center and is functionalized to selectively interact with a second analyte in the environment, wherein the functions further comprise:
 operating the light source to expose the environment to additional illumination, and wherein the additional illumination causes the nitrogen vacancy centers to emit light;   operating the light sensor to detect one or more properties of the light emitted by the nitrogen vacancy centers in response to the additional illumination; and   determining whether a nanodiamond in the environment was a silicon-vacancy nanodiamond or a nitrogen-vacancy nanodiamond based on at least the detected one or more properties of the light emitted by the silicon vacancy centers and the detected one or more properties of the light emitted by the nitrogen vacancy centers.   
     
     
         40 . The device of  claim 39 , wherein each of the silicon-vacancy nanodiamonds has at least one nitrogen vacancy center, wherein the ratio of the concentration of silicon vacancy centers to the concentration of nitrogen vacancy centers in the silicon-vacancy nanodiamonds is a first ratio, wherein each of the nitrogen-vacancy nanodiamonds has at least one silicon vacancy center, wherein the ratio of the concentration of silicon vacancy centers to the concentration of nitrogen vacancy centers in the nitrogen-vacancy nanodiamonds is a second ratio, wherein the first and second ratios are different.