Membrane phase electrode using printing and bio-molecule detection using same

Abstract

A membrane electrode includes a novel sensor combining a filtering function of a membrane and a signal measuring ability of an electrode. A target material may be measured by filtration through the membrane. A small amount of target materials may be detected with high sensitivity using an amplified electrical signal by increasing electrical conductivity by reducing metal ions on the membrane, and thus the target material may be subject to quantitative analysis. In addition, only a target material selectively binding to a receptor may be filtrated by passing a sample through the membrane after a receptor material is fixed to the electrode, and thus may be used to detect an electrical signal. In addition, the sensor may measure a signal in various methods such as electrical conductivity, impedance, etc.

1 . A sensor, comprising:
 a filtering membrane for filtering a reaction solution; and   an electrode placed on the filtering membrane;   wherein the reaction solution is prepared by mixing a ligand-fixed enzyme or ligand-fixed metal nanoparticle and a sample containing a target material,   wherein the ligand-fixed enzyme or the ligand-fixed metal nanoparticles bound with the target material remain on the filtering membrane,   wherein the electrode generates an electrical signal by the ligand-fixed enzyme or the ligand-fixed metal nanoparticles bound with the target material.   
     
     
         2 . The sensor according to  claim 1 ,
 wherein the filtering membrane is a filter formed of one selected from the group consisting of nitrocellulose, polycarbonate, nylon, polyester, cellulose acetate, polysulfone, and polyethanesulfone.   
     
     
         3 . The sensor according to  claim 1 ,
 wherein the electrode is printed by a screen printing process.   
     
     
         4 . The sensor according to  claim 3 ,
 wherein the electrode is an interdigitated electrode having a gap of 10 to 1000 μm.   
     
     
         5 . The sensor according to  claim 1 ,
 wherein the ligand is an antibody, an antigen, an enzyme, a peptide, a protein, DNA, RNA, PNA, or an aptamer.   
     
     
         6 . The sensor according to  claim 1 ,
 wherein the enzyme is a peroxidase, an alkali phosphatase, a galactosidase, or a glucose oxidase.   
     
     
         7 . The sensor according to  claim 1 ,
 wherein the nanoparticle is a gold, silver, copper or magnetic nanoparticle.   
     
     
         8 . The sensor according to  claim 1 ,
 wherein the target material is a microorganism, an antigen, a nucleic acid, a cell or an organ of an animal or plant.   
     
     
         9 . The sensor according to  claim 1 ,
 wherein a receptor is further fixed on the filtering membrane between the electrodes.   
     
     
         10 . The sensor according to  claim 1 ,
 wherein a metal is further placed on a surface of the electrode,   wherein the electrical signal is amplified by increased electrical conductivity due to the metal.   
     
     
         11 - 17 . (canceled)