Bone grafts and methods of making and using bone grafts

Abstract

Provided herein are bone grafts and methods of making and using the same, as well as products and kits that include such bone grafts. In particular, bone grafts are provided that include collagen Type I and one or more different types of mineral compositions having different dissolution properties and/or sizes, to enhance bone regeneration throughout the bone healing phase.

We claim: 
     
         1 . A bone graft comprising:
 20% to 95% by weight of collagen type I with respect to a total weight of the bone graft, and   20% to 95% by weight of one or more minerals with respect to the total weight of the bone graft, wherein the one or more minerals have two or more dissolution rates, thereby allowing the bone graft to enhance bone healing in both early and late phases of bone healing.   
     
     
         2 . The bone graft of  claim 1 , further comprising 0.2 to 20% by weight of hyaluronic acid with respect to the total weight of the bone graft. 
     
     
         3 . The bone graft of  claim 1 , further comprising 0.1 to 20% by weight of acid or base with respect to the total weight of the bone graft. 
     
     
         4 . The bone graft of  claim 1 , further comprising 20% to 95% by weight DBM with respect to the total weight of the bone graft. 
     
     
         5 . The bone graft of  claim 1 , wherein the collagen type I is from a bovine or porcine source and is obtained from either skin (dermal) or tendon. 
     
     
         6 . The bone graft of  claim 1 , wherein the one or more minerals comprise beta-tricalcium phosphate. 
     
     
         7 . The bone graft of  claim 1 , wherein the one or more minerals comprise carbonate apatite. 
     
     
         8 . The bone graft of  claim 1 , wherein the one or more minerals comprise calcium carbonate. 
     
     
         9 . The bone graft of  claim 1 , wherein the one or more minerals have a size ranging from about 75 nm to 500 μm. 
     
     
         10 . The bone graft of  claim 1 , wherein the one or more minerals include a first mineral having a first dissolution profile and a second mineral having a second dissolution profile, wherein the first dissolution profile is different from the second dissolution profile in order to provide two or more different dissolution rates. 
     
     
         11 . The bone graft of  claim 1 , wherein the bone graft is cross-linked. 
     
     
         12 . A bone graft comprising:
 20% to 95% by weight of collagen type I with respect to a total weight of the bone graft, and   20% to 95% by weight of one or more minerals with respect to the total weight of the bone graft, wherein the one or more minerals have different dissolution properties and/or sizes than one another.   
     
     
         13 . The bone graft of  claim 12 , further comprising 0.2 to 20% by weight of hyaluronic acid with respect to the total weight of the bone graft. 
     
     
         14 . The bone graft of  claim 12 , further comprising 0.1 to 20% by weight of acid or base with respect to the total weight of the bone graft. 
     
     
         15 . The bone graft of  claim 12 , further comprising 20% to 95% by weight DBM with respect to the total weight of the bone graft. 
     
     
         16 . The bone graft of  claim 12 , wherein the collagen type I is from bovine or porcine source and is obtained from either skin (dermal) or tendon. 
     
     
         17 . The bone graft of  claim 12 , wherein the one or more minerals comprise beta-tricalcium phosphate. 
     
     
         18 . The bone graft of  claim 12 , wherein the one or more minerals comprise carbonate apatite. 
     
     
         19 . The bone graft of  claim 12 , wherein the one or more minerals comprise calcium carbonate. 
     
     
         20 . The bone graft of  claim 12 , wherein the one or more minerals have a size ranging from about 75 nm to 500 μm.