Methods and compositions to produce rice resistant to accase inhibitors

Abstract

Mutant rice resistant/tolerant to ACCase inhibiting herbicides, in particular FOP herbicides, are listed in Table 1. The ACCase inhibiting herbicides used for selection include quizalofop. An exemplary mutant rice tolerant to an ACCase herbicide is disclosed, with a rice genome having G2096S or the equivalent, in the carboxyl transferase domain of the ACCase coding gene, using the Black-Grass numbering system. This mutation shows differential response to FOPs vs. DIMs herbicides, and a greater differential with comparable non-resistant rice lines. Methods to control weeds and methods to produce herbicide resistant rice including transgenic rice, are disclosed.

1 . An herbicide resistant rice plant comprising a DNA sequence comprising a plastidic carboxyl transferase domain of an ACCase coding gene which confers resistance to an ACCase inhibiting herbicide, wherein the herbicide is selected from the aryloxyphenoxypropanoates (“FOPs”) class of ACCase inhibiting herbicides, a rice plant with a nucleotide sequence as in positions 1680-1682 of SEQ ID NO: 1 or the codon equivalent. 
     
     
         2 . The rice plant of  claim 1  wherein the aryloryphenoryproponates herbicide is selected from the group consisting of quizalofop, clodinafop-propargyl, haloxyfop-methyl, and combinations thereof. 
     
     
         3 . An herbicide resistant/tolerant rice plant derived from a representative sample of seed selected from the group consisting of seeds deposited under ATCC Accession No. PTA-12933, ATCC Accession No. PTA-13619 and combinations thereof. 
     
     
         4 . (canceled) 
     
     
         5 . (canceled) 
     
     
         6 . A tissue culture produced from protoplasts or cells from the rice plant of  claim 1  wherein said cells or protoplasts of the tissue culture are produced from a plant part selected from the group consisting of leaves, pollen, embryos, cotyledon, hypocotyl, meristematic cells, roots, root tips, pistils, anthers, flowers, stems, glumes and panicles. 
     
     
         7 . A rice plant regenerated from the tissue culture of  claim 6 . 
     
     
         8 . A method of producing a herbicide resistant rice plant of  claim 1 , wherein the method comprises transforming a rice plant with a transgene, wherein the transgene confers resistance to an herbicide in the aryloxyphenoxypropanoates (“FOPs”) class of ACCase inhibitors, and the transgene has a nucleic acid sequence that is the coding equivalent of SEQ ID NO: 1 and wherein resistance is due to a substitution in the amino acid sequence of ACCase at position 464 of SEQ ID NO: 7, corresponding to position 2096 of the full length BlackGrass amino acid sequence. 
     
     
         9 . The method of producing a herbicide resistant rice of  claim 8 , wherein the nucleic acid molecule having SEQ ID NO: 1 comprises a codon in position 1680-1682 that encodes an amino acid substitution that makes the ACCase enzyme resistant to inhibitors, and further comprises promoters, terminators, selectable markers, or other domains of the ACCase coding gene, necessary to encode an ACCase enzyme resistant to herbicides. 
     
     
         10 . The method of  claim 8  wherein the mutation associated with resistance encodes an amino acid substitution G to S at an amino acid positions corresponding to 2096 of the full length BlackGrass ACCase amino acid sequence for ACCase. 
     
     
         11 . (canceled)