Methods for enhancing microbial production of specific length fatty alcohols in the presence of methanol

Abstract

The invention provides non-naturally occurring microbial organisms having a formaldehyde fixation pathway, a formate assimilation pathway, and/or a methanol metabolic pathway in combination with a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length or isopropanol. The microbial organisms provided advantageously enhance the production of substrates and/or pathway intermediates for the production of chain length specific fatty alcohols, fatty aldehydes, fatty acids or isopropanol. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde, a fatty acid or isopropanol.

What is claimed is: 
     
         1 . A non-naturally occurring microbial organism having:
 (i) a formaldehyde fixation pathway;   (ii) a formate assimilation pathway; and/or   (iii) a methanol metabolic pathway, and   a malonyl-CoA independent fatty acyl-CoA elongation (MI-FAE) cycle and/or a malonyl-CoA dependent fatty acyl-CoA elongation (MD-FAE) cycle in combination with a termination pathway,   wherein said formaldehyde fixation pathway comprises:   (1) 1B and 1C; (2) 1D; or (3) 1D and 1Z,   wherein 1B is a 3-hexulose-6-phosphate synthase, wherein 1C is a 6-phospho-3-hexuloisomerase, wherein 1D is a dihydroxyacetone synthase, wherein 1Z is a fructose-6-phosphate aldolase,   wherein said formate assimilation pathway comprises a pathway selected from:   (4) 1E; (5) 1F, and 1G; (6) 1H, 1I, 1J, and 1K; (7) 1H, 1I, 1J, 1L, 1M, and 1N; (8) 1E, 1H, 1I, 1J, 1L, 1M, and 1N; (9) 1F, 1G, 1H, 1I, 1J, 1L, 1M, and 1N; (10) 1K, 1H, 1I, 1J, 1L, 1M, and 1N; and (11) 1H, 1I, 1J, 1O, and 1P,   wherein 1E is a formate reductase, 1F is a formate ligase, a formate transferase, or a formate synthetase, wherein 1G is a formyl-CoA reductase, wherein 1H is a formyltetrahydrofolate synthetase, wherein 1I is a methenyltetrahydrofolate cyclohydrolase, wherein 1J is a methylenetetrahydrofolate dehydrogenase, wherein 1K is a formaldehyde-forming enzyme or spontaneous, wherein 1L is a glycine cleavage system, wherein 1M is a serine hydroxymethyltransferase, wherein 1N is a serine deaminase, wherein 1O is a methylenetetrahydrofolate reductase, wherein 1P is an acetyl-CoA synthase,   wherein said methanol metabolic pathway comprises a pathway selected from:   (12) 10J; (13) 10A; (14) 10A and 10B; (15) 10A, 10B and 10C; (16) 10J, 10K and 10C; (17) 10J, 10M, and 10N; (18) 10J and 10L; (19) 10J, 10L, and 10G; (20) 10J, 10L, and 10I; (21) 10A, 10B, 10C, 10D, and 10E; (22) 10A, 10B, 10C, 10D, and 10F; (23) 10J, 10K, 10C, 10D, and 10E; (24) 10J, 10K, 10C, 10D, and 10F; (25) 10J, 10M, 10N, and 10O; (26) 10A, 10B, 10C, 10D, 10E, and 10G; (27) 10A, 10B, 10C, 10D, 10F, and 10G; (28) 10J, 10K, 10C, 10D, 10E, and 10G; (29) 10J, 10K, 10C, 10D, 10F, and 10G; (30) 10J, 10M, 10N, 10O, and 10G; (31) 10A, 10B, 10C, 10D, 10E, and 10I; (32) 10A, 10B, 10C, 10D, 10F, and 10I; (33) 10J, 10K, 10C, 10D, 10E, and 10I; (34) 10J, 10K, 10C, 10D, 10F, and 10I; and (35) 10J, 10M, 10N, 10O, and 10I,   wherein 10A is a methanol methyltransferase, wherein 10B is a methylenetetrahydrofolate reductase, wherein 10C is a methylenetetrahydrofolate dehydrogenase, wherein 10D is a methenyltetrahydrofolate cyclohydrolase, wherein 10E is a formyltetrahydrofolate deformylase, wherein 10F is a formyltetrahydrofolate synthetase, wherein 10G is a formate hydrogen lyase, wherein 10I is a formate dehydrogenase, wherein 10J is a methanol dehydrogenase, wherein 10K is a formaldehyde activating enzyme or spontaneous, wherein 10L is a formaldehyde dehydrogenase, wherein 10M is a S-(hydroxymethyl)glutathione synthase or spontaneous, wherein 10N is a glutathione-dependent formaldehyde dehydrogenase, wherein 10O is a S-formylglutathione hydrolase,   wherein said MI-FAE cycle comprises one or more thiolase, one or more 3-oxoacyl-CoA reductase, one or more 3-hydroxyacyl-CoA dehydratase, and one or more enoyl-CoA reductase,   wherein said MD-FAE cycle comprises one or more elongase, one or more 3-oxoacyl-CoA reductase, one or more 3-hydroxyacyl-CoA dehydratase, and one or more enoyl-CoA reductase,   wherein said termination pathway comprises a pathway selected from:   (36) 2H; (37) 2K and 2L; (38) 2E and 2N; (39) 2K, 2J, and 2N; (40) 2E; (41) 2K and 2J; (42) 2H and 2N; (43) 2K, 2L, and 2N; (44) 2E and 2F; (45) 2K, 2J, and 2F; (46) 2H, 2N, and 2F; (47) 2K, 2L, 2N, and 2F; (48) 2G; and (49) 2P,   wherein 2E is an acyl-CoA reductase (aldehyde forming), wherein 2F is an alcohol dehydrogenase, wherein 2G is an acyl-CoA reductase (alcohol forming), wherein 2H is an acyl-CoA hydrolase, acyl-CoA transferase or acyl-CoA synthase, wherein 2J is an acyl-ACP reductase, wherein 2K is an acyl-CoA:ACP acyltransferase, wherein 2L is a thioesterase, wherein 2N is an aldehyde dehydrogenase (acid forming) or a carboxylic acid reductase, wherein 2P is an acyl-ACP reductase (alcohol forming),   wherein an enzyme of the formaldehyde fixation pathway, the formate assimilation pathway, the methanol metabolic pathway, the MI-FAE cycle, the MD-FAE cycle or the termination pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce a compound of Formula (I):   
       
         
           
           
               
               
           
         
         wherein R 1  is C 1-24  linear alkyl; R 2  is CH 2 OH, CHO, or COOH; R 3  is H, OH, or oxo (═O); and   represents a single or double bond with the proviso that the valency of the carbon atom to which R 3  is attached is four, 
         wherein the substrate of each of said enzymes of the MI-FAE cycle, the MD-FAE cycle and the termination pathway are independently selected from a compound of Formula (II), malonyl-CoA, propionyl-CoA or acetyl-CoA: 
       
       
         
           
           
               
               
           
         
         wherein R 1  is C 1-24  linear alkyl; R 3  is H, OH, or oxo (═O); R 4  is S-CoA, ACP, OH or H; and   represents a single or double bond with the proviso that the valency of the carbon atom to which R 3  is attached is four; 
         wherein said one or more enzymes of the MI-FAE cycle are each selective for a compound of Formula (II) having a number of carbon atoms at R 1  that is no greater than the number of carbon atoms at R 1  of said compound of Formula (I), 
         wherein said one or more enzymes of the MD-FAE cycle are each selective for a compound of Formula (II) having a number of carbon atoms at R 1  that is no greater than the number of carbon atoms at R 1  of said compound of Formula (I), and 
         wherein said one or more enzymes of the termination pathway are each selective for a compound of Formula (II) having a number of carbon atoms at R 1  that is no less than the number of carbon atoms at R 1  of said compound of Formula (I). 
       
     
     
         2 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism has a formaldehyde fixation pathway and a MI-FAE cycle in combination with a termination pathway. 
     
     
         3 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism has a formate assimilation pathway and a MI-FAE cycle in combination with a termination pathway. 
     
     
         4 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, and a MI-FAE cycle in combination with a termination pathway. 
     
     
         5 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism has a formaldehyde fixation pathway and a MD-FAE cycle in combination with a termination pathway. 
     
     
         6 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism has a formate assimilation pathway and a MD-FAE cycle in combination with a termination pathway. 
     
     
         7 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, and a MD-FAE cycle in combination with a termination pathway. 
     
     
         8 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism has a methanol metabolic pathway and a MI-FAE cycle in combination with a termination pathway. 
     
     
         9 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism has a methanol metabolic pathway and a MD-FAE cycle in combination with a termination pathway. 
     
     
         10 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism has a formaldehyde fixation pathway, a methanol metabolic pathway and a MI-FAE cycle in combination with a termination pathway. 
     
     
         11 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism has a formate assimilation pathway, a methanol metabolic pathway and a MI-FAE cycle in combination with a termination pathway. 
     
     
         12 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, a methanol metabolic pathway and a MI-FAE cycle in combination with a termination pathway. 
     
     
         13 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism has a formaldehyde fixation pathway, a methanol metabolic pathway and a MD-FAE cycle in combination with a termination pathway. 
     
     
         14 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism has a formate assimilation pathway, a methanol metabolic pathway and a MD-FAE cycle in combination with a termination pathway. 
     
     
         15 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, a methanol metabolic pathway and MD-FAE cycle in combination with a termination pathway. 
     
     
         16 . The non-naturally occurring microbial organism of any one of  claims 1 - 15 , wherein R 1  is C 1-17  linear alkyl. 
     
     
         17 . The non-naturally occurring microbial organism of  claim 16 , wherein R 1  is C 9  linear alkyl, C 10  linear alkyl, C 11 , linear alkyl, C 12  linear alkyl or C 13  linear alkyl. 
     
     
         18 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism comprises two, three, or four exogenous nucleic acids each encoding an enzyme of said MI-FAE cycle or said MD-FAE cycle. 
     
     
         19 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism comprises two, three, or four exogenous nucleic acids each encoding an enzyme of said termination pathway. 
     
     
         20 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism comprises one, two, three, four, five, six, seven, or eight exogenous nucleic acids each encoding a formaldehyde fixation pathway enzyme, a formate assimilation pathway enzyme, or a methanol metabolic pathway enzyme. 
     
     
         21 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism comprises exogenous nucleic acids encoding each of the enzymes of at least one of the pathways selected from (1)-(49). 
     
     
         22 . The non-naturally occurring microbial organism of any one of  claims 1 - 21 , wherein said formate assimilation pathway further comprises:
 (1) 1Q; (2) 1R, and 1S; (3) 1Y and 1Q; or (4) 1Y, 1R and 1S,   wherein 1Q is a pyruvate formate lyase, wherein 1R is a pyruvate dehydrogenase, a pyruvate ferredoxin oxidoreductase, or a pyruvate:NADP+ oxidoreductase, wherein 1S is a formate dehydrogenase, wherein 1Y is a glyceraldehyde-3-phosphate dehydrogenase or an enzyme of lower glycolysis.   
     
     
         23 . The non-naturally occurring microbial organism of any one of  claims 1 - 22 , wherein said organism further comprises a methanol oxidation pathway. 
     
     
         24 . The non-naturally occurring microbial organism of  claim 23 , wherein said organism comprises at least one exogenous nucleic acid encoding a methanol oxidation pathway enzyme expressed in a sufficient amount to produce formaldehyde in the presence of methanol, wherein said methanol oxidation pathway comprises 1A, wherein 1A a methanol dehydrogenase. 
     
     
         25 . The non-naturally occurring microbial organism of any one of  claims 1 - 24 , wherein said microbial organism further comprises 3H or 3P, wherein 3H is a hydrogenase, wherein 3P a carbon monoxide dehydrogenase. 
     
     
         26 . The non-naturally occurring micorobial organism of  claim 25 , wherein organism comprises an exogenous nucleic acid encoding said hydrogenase or said carbon monoxide dehydrogenase. 
     
     
         27 . The non-naturally occurring microbial organism of any one of  claims 1 - 26 , wherein said at least one exogenous nucleic acid encoding said formaldehyde fixation pathway enzyme, said formate assimilation pathway enzyme, said methanol metabolic pathway enzyme, said MI-FAE cycle enzyme, said MD-FAE cycle enzyme, said termination pathway enzyme, said methanol oxidation pathway enzyme, said hydrogenase or said carbon monoxide dehydrogenase is a heterologous nucleic acid. 
     
     
         28 . The non-naturally occurring microbial organism of any one of  claims 1 - 27 , wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium. 
     
     
         29 . The non naturally occurring microbial organism of  claim 1 , wherein said enzyme of the formaldehyde fixation pathway, formate assimilation pathway, methanol metabolic pathway, MI-FAE cycle, MD-FAE cycle or termination pathway is expressed in a sufficient amount to produce a compound selected from the Formulas (III)-(VI): 
       
         
           
           
               
               
           
         
         wherein R 1  is C 1-17  linear alkyl. 
       
     
     
         30 . The non-naturally occurring microbial organism of  claim 29 , wherein R 1  is C 9  linear alkyl, C 10  linear alkyl, C 11 , linear alkyl, C 12  linear alkyl or C 13  linear alkyl. 
     
     
         31 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism further comprises an acetyl-CoA pathway and at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme expressed in a sufficient amount to produce or enhance carbon flux through acetyl-CoA, wherein said acetyl-CoA pathway comprises a pathway selected from:
 (1) 3A and 3B; (2) 3A, 3C, and 3D; (3) 3H; (4) 3G and 3D; (5) 3E, 3F and 3B; (6) 3E and 3I; (7) 3J, 3F and 3B; (8) 3J and 3I; (9) 4A, 4B, and 4C; (10) 4A, 4B, 4J, 4K, and 4D; (11) 4A, 4B, 4G, and 4D; (12) 4A, 4F, and 4D; (13) 4N, 4H, 4B and 4C; (14) 4N, 4H, 4B, 4J, 4K, and 4D; (15) 4N, 4H, 4B, 4G, and 4D; (16) 4N, 4H, 4F, and 4D; (17) 4L, 4M, 4B and 4C; (18) 4L, 4M, 4B, 4J, 4K, and 4D; (19) 4L, 4M, 4B, 4G, and 4D; (20) 4L, 4M, 4F, and 4D; (21) 5A, 5B, 5D, 5H, 5I, and 5J; (22) 5A, 5B, 5E, 5F, 5H, 5I, and 5J; (23) 5A, 5B, 5E, 5K, 5L, 5H, 5I, and 5J; (24) 5A, 5C, 5D, 5H, and 5J; (25) 5A, 5C, 5E, 5F, 5H, and 5J; (26) 5A, 5C, 5E, 5K, 5L, 5H, and 5J; (27) 6A, 6B, 6D, and 6G; (28) 6A, 6B, 6E, 6F, and 6G; (29) 6A, 6B, 6E, 6K, 6L, and 6G; (30) 6A, 6C, and 6D; (31) 6A, 6C, 6E, and 6F; (32) 6A, 6C, 6E, 6K, and 6L; (33) 1T and 1V; (34) 1T, 1W, and 1X; (35) 1U and 1V; and (36) 1U, 1W, and 1X,   wherein 3A is a pyruvate oxidase (acetate-forming), wherein 3B is an acetyl-CoA synthetase, an acetyl-CoA ligase or an acetyl-CoA transferase, wherein 3C is an acetate kinase, wherein 3D is a phosphotransacetylase, wherein 3E is a pyruvate decarboxylase, wherein 3F is an acetaldehyde dehydrogenase, wherein 3G is a pyruvate oxidase (acetyl-phosphate forming), wherein 3H is a pyruvate dehydrogenase, a pyruvate:ferredoxin oxidoreductase, a pyruvate:NAD(P)H oxidoreductase or a pyruvate formate lyase, wherein 3I is an acetaldehyde dehydrogenase (acylating), wherein 3J is a threonine aldolase, wherein 4A is a phosphoenolpyruvate (PEP) carboxylase or a PEP carboxykinase, wherein 4B is an oxaloacetate decarboxylase, wherein 4C is a malonate semialdehyde dehydrogenase (acetylating), wherein 4D is an acetyl-CoA carboxylase or a malonyl-CoA decarboxylase, wherein 4F is an oxaloacetate dehydrogenase or an oxaloacetate oxidoreductase, wherein 4G is a malonate semialdehyde dehydrogenase (acylating), wherein 4H is a pyruvate carboxylase, wherein 4J is a malonate semialdehyde dehydrogenase, wherein 4K is a malonyl-CoA synthetase or a malonyl-CoA transferase, wherein 4L is a malic enzyme, wherein 4M is a malate dehydrogenase or a malate oxidoreductase, wherein 4N is a pyruvate kinase or a PEP phosphatase, wherein 5A is a citrate synthase, wherein 5B is a citrate transporter, wherein 5C is a citrate/malate transporter, wherein 5D is an ATP citrate lyase, wherein 5E is a citrate lyase, wherein 5F is an acetyl-CoA synthetase or an acetyl-CoA transferase, wherein 5H is a cytosolic malate dehydrogenase, wherein 5I is a malate transporter, wherein 5J is a mitochondrial malate dehydrogenase, wherein 5K is an acetate kinase, wherein 5L is a phosphotransacetylase, wherein 6A is a citrate synthase, wherein 6B is a citrate transporter, wherein 6C is a citrate/oxaloacetate transporter, wherein 6D is an ATP citrate lyase, wherein 6E is a citrate lyase, wherein 6F is an acetyl-CoA synthetase or an acetyl-CoA transferase, wherein 6G is an oxaloacetate transporter, wherein 6K is an acetate kinase, wherein 6L is a phosphotransacetylase, wherein 1T is a fructose-6-phosphate phosphoketolase, wherein 1U is a xylulose-5-phosphate phosphoketolase, wherein 1V is a phosphotransacetylase, wherein 1W is an acetate kinase, wherein 1X is an acetyl-CoA transferase, an acetyl-CoA synthetase, or an acetyl-CoA ligase.   
     
     
         32 . The non-naturally occurring microbial organism of  claim 31 , wherein said microbial organism comprises two, three, four, five, six, seven or eight exogenous nucleic acids each encoding an acetyl-CoA pathway enzyme. 
     
     
         33 . The non-naturally occurring microbial organism of  claim 32 , wherein said microbial organism comprises exogenous nucleic acids encoding each of the acetyl-CoA pathway enzymes of at least one of the pathways selected from (1)-(36). 
     
     
         34 . The non-naturally occurring microbial organism of  claim 1 , further comprising one or more gene disruptions, said one or more gene disruptions occurring in endogenous genes encoding proteins or enzymes involved in: native production of ethanol, glycerol, acetate, formate, lactate, CO 2 , fatty acids, or malonyl-CoA by said microbial organism; transfer of pathway intermediates to cellular compartments other than the cytosol; or native degradation of a MI-FAE cycle intermediate, MD-FAE cycle intermediate or a termination pathway intermediate by said microbial organism, wherein said one or more gene disruptions confer increased production of the compound of Formula (I) in said microbial organism. 
     
     
         35 . The non-naturally occurring microbial organism of  claim 34 , wherein said protein or enzyme is selected from the group consisting of a fatty acid synthase, an acetyl-CoA carboxylase, a biotin:apoenzyme ligase, an acyl carrier protein, a thioesterase, an acyltransferase, an ACP malonyltransferase, a fatty acid elongase, an acyl-CoA synthetase, an acyl-CoA transferase, an acyl-CoA hydrolase, a pyruvate decarboxylase, a lactate dehydrogenase, an alcohol dehydrogenase, an acid-forming aldehyde dehydrogenases, an acetate kinase, a phosphotransacetylase, a pyruvate oxidase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate phosphatase, a mitochondrial pyruvate carrier, a peroxisomal fatty acid transporter, a peroxisomal acyl-CoA transporter, a peroxisomal carnitine/acylcarnitine transferase, an acyl-CoA oxidase, and an acyl-CoA binding protein. 
     
     
         36 . The non-naturally occurring microbial organism of  claim 1 , wherein one or more enzymes of the MI-FAE cycle, MD-FAE cycle or the termination pathway preferentially react with an NADH cofactor or have reduced preference for reacting with an NAD(P)H cofactor, wherein said one or more enzymes of the MI-FAE cycle or MD-FAE cycle are a 3-ketoacyl-CoA reductase or an enoyl-CoA reductase, and wherein said one or more enzymes of the termination pathway are selected from an acyl-CoA reductase (aldehyde forming), an alcohol dehydrogenase, an acyl-CoA reductase (alcohol forming), an aldehyde decarbonylase, an acyl-ACP reductase, an aldehyde dehydrogenase (acid forming) and a carboxylic acid reductase. 
     
     
         37 . The non-naturally occurring microbial organism of  claim 1 , further comprising one or more gene disruptions, said one or more gene disruptions occurring in genes encoding proteins or enzymes that result in an increased ratio of NAD(P)H to NAD(P) present in the cytosol of said microbial organism following said disruptions. 
     
     
         38 . The non-naturally occurring microbial organism of  claim 37 , wherein said gene encoding a protein or enzyme that results in an increased ratio of NAD(P)H to NAD(P) present in the cytosol of said microbial organism following said disruptions is selected from the group consisting of an NADH dehydrogenase, a cytochrome oxidase, a glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate phosphatase, an alcohol dehydrogenase, a pyruvate decarboxylase, an aldehyde dehydrogenase (acid forming), a lactate dehydrogenase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate:quinone oxidoreductase, a malic enzyme and a malate dehydrogenase. 
     
     
         39 . The non-naturally occurring organism of  claim 34  or  37 , wherein said one or more gene disruptions comprises a deletion of said one or more genes. 
     
     
         40 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism is Crabtree positive and is in culture medium comprising excess glucose, thereby increasing the ratio of NAD(P)H to NAD(P) present in the cytosol of said microbial organism. 
     
     
         41 . The non-naturally occurring microbial organism of  claim 1 , further comprising at least one exogenous nucleic acid encoding an extracellular transporter or an extracellular transport system for the compound of Formula (I). 
     
     
         42 . The non-naturally occurring microbial organism of  claim 1 , wherein one or more endogenous enzymes involved in: native production of ethanol, glycerol, acetate, formate, lactate, CO2, fatty acids, or malonyl-CoA by said microbial organism; transfer of pathway intermediates to cellular compartments other than the cytosol; or native degradation of a MI-FAE cycle intermediate, MD-FAE cycle intermediate or a termination pathway intermediate by said microbial organism, has attenuated enzyme activity or expression levels. 
     
     
         43 . The non-naturally occurring microbial organism of  claim 42 , wherein said enzyme is selected from the group consisting of a fatty acid synthase, an acetyl-CoA carboxylase, a biotin:apoenzyme ligase, a thioesterase, an acyl carrier protein, a thioesterase, an acyltransferase, an ACP malonyltransferase, a fatty acid elongase, an acyl-CoA synthetase, an acyl-CoA transferase, an acyl-CoA hydrolase, a pyruvate decarboxylase, a lactate dehydrogenase, a short-chain alcohol dehydrogenase, an acid-forming aldehyde dehydrogenase, an acetate kinase, a phosphotransacetylase, a pyruvate oxidase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate phosphatase, a mitochondrial pyruvate carrier, a peroxisomal fatty acid transporter, a peroxisomal acyl-CoA transporter, a peroxisomal carnitine/acylcarnitine transferase, an acyl-CoA oxidase, and an acyl-CoA binding protein. 
     
     
         44 . The non-naturally occurring microbial organism of  claim 1 , wherein one or more endogenous enzymes involved in the oxidation of NAD(P)H or NADH, has attenuated enzyme activity or expression levels. 
     
     
         45 . The non-naturally occurring microbial organism of  claim 44 , wherein said one or more endogenous enzymes are selected from the group consisting of an NADH dehydrogenase, a cytochrome oxidase, a glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate phosphatase, an alcohol dehydrogenase, a pyruvate decarboxylase, an aldehyde dehydrogenase (acid forming), a lactate dehydrogenase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate:quinone oxidoreductase, a malic enzyme and a malate dehydrogenase. 
     
     
         46 . A method for producing a compound of Formula (I): 
       
         
           
           
               
               
           
         
         wherein R 1  is C 1-24  linear alkyl; R 2  is CH 2 OH, CHO, or COOH; R 3  is H, OH, or oxo (═O); and   represents a single or double bond with the proviso that the valency of the carbon atom to which R 3  is attached is four, comprising culturing the non-naturally occurring microbial organism of any one of  claims 1 - 45  under conditions for a sufficient period of time to produce said compound of Formula (I). 
       
     
     
         47 . The method of  claim 46 , wherein said method further comprises separating the compound of Formula (I) from other components in the culture. 
     
     
         48 . The method of  claim 47 , wherein the separating comprises extraction, continuous liquid-liquid extraction, pervaporation, membrane filtration, membrane separation, reverse osmosis, electrodialysis, distillation, crystallization, centrifugation, extractive filtration, ion exchange chromatography, absorption chromatography, or ultrafiltration. 
     
     
         49 . Culture medium comprising bioderived compound of Formula (I): 
       
         
           
           
               
               
           
         
         wherein R 1  is C 1-24  linear alkyl; R 2  is CH 2 OH, CHO, or COOH; R 3  is H, OH, or oxo (═O); and   represents a single or double bond with the proviso that the valency of the carbon atom to which R 3  is attached is four, wherein said culture medium is separated from a non-naturally occurring microbial organism of any one of  claims 1 - 45 . 
       
     
     
         50 . A bioderived compound of Formula (I): 
       
         
           
           
               
               
           
         
         wherein R 1  is C 1-24  linear alkyl; R 2  is CH 2 OH, CHO, or COOH; R 3  is H, OH, or oxo (═O); and   represents a single or double bond with the proviso that the valency of the carbon atom to which R 3  is attached is four, wherein said bioderived compound is produced according to the method of any one of  claims 46 - 48 . 
       
     
     
         51 . The bioderived compound of  claim 50 , wherein said bioderived compound has an Fm value of at least 80%, at least 85%, at least 90%, at least 95% or at least 98%. 
     
     
         52 . A composition comprising said bioderived compound of  claim 50  or  51  and a compound other than said bioderived compound. 
     
     
         53 . The composition of  claim 52  wherein said compound other than said bioderived compound is a trace amount of a cellular portion of a non-naturally occurring microbial organism having:
 (i) a formaldehyde fixation pathway; 
 (ii) a formate assimilation pathway; and/or 
 (iii) a methanol metabolic pathway, and 
 a malonyl-CoA independent fatty acyl-CoA elongation (MI-FAE) cycle and/or a malonyl-CoA dependent fatty acyl-CoA elongation (MD-FAE) cycle in combination with a termination pathway. 
 
     
     
         54 . A composition comprising the bioderived compound of  claim 50  or  51 , or a cell lysate or culture supernatant thereof. 
     
     
         55 . A biobased product comprising said bioderived compound of  claim 50  or  51 , wherein said biobased product is a biofuel, chemical, polymer, surfactant, soap, detergent, shampoo, lubricating oil additive, fragrance, flavor material or acrylate. 
     
     
         56 . The biobased product of  claim 55  comprising at least 5%, at least 10%, at least 20%, at least 30%, at least 40% or at least 50% said bioderived compound. 
     
     
         57 . The biobased product of  claim 55  or  56 , wherein said biobased product comprises a portion of said bioderived compound as a repeating unit. 
     
     
         58 . A molded product obtained by molding a biobased product of any one of  claims 55 - 57 , wherein said biobased product is a polymer. 
     
     
         59 . A process for producing a biobased product of any one of  claims 55 - 57  comprising chemically reacting said bioderived compound with itself or another compound in a reaction that produces said biobased product. 
     
     
         60 . A non-naturally occurring microbial organism having:
 (i) a formaldehyde fixation pathway;   (ii) a formate assimilation pathway; and/or   (iii) a methanol metabolic pathway, and   an isopropanol pathway,   wherein said formaldehyde fixation pathway comprises:   (1) 1B and 1C; (2) 1D; (3) 1D and 1Z,   wherein 1B is a 3-hexulose-6-phosphate synthase, wherein 1C is a 6-phospho-3-hexuloisomerase, wherein 1D is a dihydroxyacetone synthase, wherein 1Z is a fructose-6-phosphate aldolase,   wherein said formate assimilation pathway comprises a pathway selected from:   (4) 1E; (5) 1F, and 1G; (6) 1H, 1I, 1J, and 1K; (7) 1H, 1I, 1J, 1L, 1M, and 1N; (8) 1E, 1H, 1I, 1J, 1L, 1M, and 1N; (9) 1F, 1G, 1H, 1I, 1J, 1L, 1M, and 1N; (10) 1K, 1H, 1I, 1J, 1L, 1M, and 1N; and (11) 1H, 1I, 1J, 1O, and 1P,   wherein 1E is a formate reductase, 1F is a formate ligase, a formate transferase, or a formate synthetase, wherein 1G is a formyl-CoA reductase, wherein 1H is a formyltetrahydrofolate synthetase, wherein 1I is a methenyltetrahydrofolate cyclohydrolase, wherein 1J is a methylenetetrahydrofolate dehydrogenase, wherein 1K is a formaldehyde-forming enzyme or spontaneous, wherein 1L is a glycine cleavage system, wherein 1M is a serine hydroxymethyltransferase, wherein 1N is a serine deaminase, wherein 1O is a methylenetetrahydrofolate reductase, wherein 1P is an acetyl-CoA synthase,   wherein said methanol metabolic pathway comprises a pathway selected from:   (12) 10J; (13) 10A; (14) 10A and 10B; (15) 10A, 10B and 10C; (16) 10J, 10K and 10C; (17) 10J, 10M, and 10N; (18) 10J and 10L; (19) 10J, 10L, and 10G; (20) 10J, 10L, and 10I; (21) 10A, 10B, 10C, 10D, and 10E; (22) 10A, 10B, 10C, 10D, and 10F; (23) 10J, 10K, 10C, 10D, and 10E; (24) 10J, 10K, 10C, 10D, and 10F; (25) 10J, 10M, 10N, and 10O; (26) 10A, 10B, 10C, 10D, 10E, and 10G; (27) 10A, 10B, 10C, 10D, 10F, and 10G; (28) 10J, 10K, 10C, 10D, 10E, and 10G; (29) 10J, 10K, 10C, 10D, 10F, and 10G; (30) 10J, 10M, 10N, 10O, and 10G; (31) 10A, 10B, 10C, 10D, 10E, and 10I; (32) 10A, 10B, 10C, 10D, 10F, and 10I; (33) 10J, 10K, 10C, 10D, 10E, and 10I; (34) 10J, 10K, 10C, 10D, 10F, and 10I; and (35) 10J, 10M, 10N, 10O, and 10I,   wherein 10A is a methanol methyltransferase, wherein 10B is a methylenetetrahydrofolate reductase, wherein 10C is a methylenetetrahydrofolate dehydrogenase, wherein 10D is a methenyltetrahydrofolate cyclohydrolase, wherein 10E is a formyltetrahydrofolate deformylase, wherein 10F is a formyltetrahydrofolate synthetase, wherein 10G is a formate hydrogen lyase, wherein 10I is a formate dehydrogenase, wherein 10J is a methanol dehydrogenase, wherein 10K is a formaldehyde activating enzyme or spontaneous, wherein 10L is a formaldehyde dehydrogenase, wherein 10M is a S-(hydroxymethyl)glutathione synthase or spontaneous, wherein 10N is a glutathione-dependent formaldehyde dehydrogenase, wherein 10O is a S-formylglutathione hydrolase,   wherein said isopanol pathway comprises:   (36) 11V, 11W, 11X, and 11Y; or (37) 11T, 11U, 11W, 11X, and 11Y,   wherein 11T is an acetyl-CoA carboxylase, wherein 11U is an acetoacetyl-CoA synthase, wherein 11V is an acetyl-CoA:acetyl-CoA acyltransferase, wherein 11W is an acetoacetyl-CoA hydrolase, an acetoacetyl-CoA transferase, an acetoacetyl-CoA ligase, or a phosphotransacetoacetylase/acetoacetate kinase, wherein 11X is an acetoacetate decarboxylase, wherein 11Y is an acetone reductase or isopropanol dehydrogenase,   wherein an enzyme of the formaldehyde fixation pathway, formate assimilation pathway, methanol metabolic pathway, or isopropanol pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce isopropanol.   
     
     
         61 . The non-naturally occurring microbial organism of  claim 60 , wherein said microbial organism has a formaldehyde fixation pathway and an isopropanol pathway. 
     
     
         62 . The non-naturally occurring microbial organism of  claim 60 , wherein said microbial organism has a formate assimilation pathway and an isopropanol pathway. 
     
     
         63 . The non-naturally occurring microbial organism of  claim 60 , wherein said microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, and an isopropanol pathway. 
     
     
         64 . The non-naturally occurring microbial organism of  claim 60 , wherein said microbial organism has a methanol metabolic pathway and an isopropanol pathway. 
     
     
         65 . The non-naturally occurring microbial organism of  claim 60 , wherein said microbial organism has a formaldehyde fixation pathway, a methanol metabolic pathway and an isopropanol pathway. 
     
     
         66 . The non-naturally occurring microbial organism of  claim 60 , wherein said microbial organism has a formate assimilation pathway, a methanol metabolic pathway and an isopropanol pathway. 
     
     
         67 . The non-naturally occurring microbial organism of  claim 60 , wherein said microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, a methanol metabolic pathway and an isopropanol pathway. 
     
     
         68 . The non-naturally occurring microbial organism of  claim 60 , wherein said microbial organism comprises two, three, four, five or six exogenous nucleic acids each encoding an enzyme of said isopropanol pathway. 
     
     
         69 . The non-naturally occurring microbial organism of  claim 60 , wherein said microbial organism comprises one, two, three, four, five, six, seven, or eight exogenous nucleic acids each encoding a formaldehyde fixation pathway enzyme, a formate assimilation pathway enzyme, or a methanol metabolic pathway enzyme. 
     
     
         70 . The non-naturally occurring microbial organism of  claim 60 , wherein said microbial organism comprises exogenous nucleic acids encoding each of the enzymes of at least one of the pathways selected from (1)-(37). 
     
     
         71 . The non-naturally occurring microbial organism of any one of  claims 60 - 70 , wherein said formate assimilation pathway further comprises:
 (1) 1Q; (2) 1R, and 1S; (3) 1Y and 1Q; or (4) 1Y, 1R and 1S,   wherein 1Q is a pyruvate formate lyase, wherein 1R is a pyruvate dehydrogenase, a pyruvate ferredoxin oxidoreductase, or a pyruvate:NADP+ oxidoreductase, wherein 1S is a formate dehydrogenase, wherein 1Y is a glyceraldehyde-3-phosphate dehydrogenase or an enzyme of lower glycolysis.   
     
     
         72 . The non-naturally occurring microbial organism of any one of  claims 60 - 71 , wherein said organism further comprises a methanol oxidation pathway. 
     
     
         73 . The non-naturally occurring microbial organism of  claim 72 , wherein said organism comprises at least one exogenous nucleic acid encoding a methanol oxidation pathway enzyme expressed in a sufficient amount to produce formaldehyde in the presence of methanol, wherein said methanol oxidation pathway comprises 1A, wherein 1A a methanol dehydrogenase. 
     
     
         74 . The non-naturally occurring microbial organism of any one of  claims 60 - 73 , wherein said microbial organism further comprises 3H or 3P, wherein 3H is a hydrogenase, wherein 3P a carbon monoxide dehydrogenase. 
     
     
         75 . The non-naturally occurring micorobial organism of  claim 74 , wherein organism comprises an exogenous nucleic acid encoding said hydrogenase or said carbon monoxide dehydrogenase. 
     
     
         76 . The non-naturally occurring microbial organism of any one of  claims 60 - 75 , wherein said at least one exogenous nucleic acid encoding said formaldehyde fixation pathway enzyme, said formate assimilation pathway enzyme, said methanol metabolic pathway enzyme, said isopropanol pathway, said methanol oxidation pathway enzyme, said hydrogenase or said carbon monoxide dehydrogenase is a heterologous nucleic acid. 
     
     
         77 . The non-naturally occurring microbial organism of any one of  claims 60 - 76 , wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium. 
     
     
         78 . The non-naturally occurring microbial organism of  claim 60 , wherein said microbial organism further comprises an acetyl-CoA pathway and at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme expressed in a sufficient amount to produce or enhance carbon flux through acetyl-CoA, wherein said acetyl-CoA pathway comprises a pathway selected from:
 (1) 3A and 3B; (2) 3A, 3C, and 3D; (3) 3H; (4) 3G and 3D; (5) 3E, 3F and 3B; (6) 3E and 3I; (7) 3J, 3F and 3B; (8) 3J and 3I; (9) 4A, 4B, and 4C; (10) 4A, 4B, 4J, 4K, and 4D; (11) 4A, 4B, 4G, and 4D; (12) 4A, 4F, and 4D; (13) 4N, 4H, 4B and 4C; (14) 4N, 4H, 4B, 4J, 4K, and 4D; (15) 4N, 4H, 4B, 4G, and 4D; (16) 4N, 4H, 4F, and 4D; (17) 4L, 4M, 4B and 4C; (18) 4L, 4M, 4B, 4J, 4K, and 4D; (19) 4L, 4M, 4B, 4G, and 4D; (20) 4L, 4M, 4F, and 4D; (21) 5A, 5B, 5D, 5H, 5I, and 5J; (22) 5A, 5B, 5E, 5F, 5H, 5I, and 5J; (23) 5A, 5B, 5E, 5K, 5L, 5H, 5I, and 5J; (24) 5A, 5C, 5D, 5H, and 5J; (25) 5A, 5C, 5E, 5F, 5H, and 5J; (26) 5A, 5C, 5E, 5K, 5L, 5H, and 5J; (27) 6A, 6B, 6D, and 6G; (28) 6A, 6B, 6E, 6F, and 6G; (29) 6A, 6B, 6E, 6K, 6L, and 6G; (30) 6A, 6C, and 6D; (31) 6A, 6C, 6E, and 6F; (32) 6A, 6C, 6E, 6K, and 6L; (33) 1T and 1V; (34) 1T, 1W, and 1X; (35) 1U and 1V; and (36) 1U, 1W, and 1X,   wherein 3A is a pyruvate oxidase (acetate-forming), wherein 3B is an acetyl-CoA synthetase, an acetyl-CoA ligase or an acetyl-CoA transferase, wherein 3C is an acetate kinase, wherein 3D is a phosphotransacetylase, wherein 3E is a pyruvate decarboxylase, wherein 3F is an acetaldehyde dehydrogenase, wherein 3G is a pyruvate oxidase (acetyl-phosphate forming), wherein 3H is a pyruvate dehydrogenase, a pyruvate:ferredoxin oxidoreductase, a pyruvate:NAD(P)H oxidoreductase or a pyruvate formate lyase, wherein 3I is an acetaldehyde dehydrogenase (acylating), wherein 3J is a threonine aldolase, wherein 4A is a phosphoenolpyruvate (PEP) carboxylase or a PEP carboxykinase, wherein 4B is an oxaloacetate decarboxylase, wherein 4C is a malonate semialdehyde dehydrogenase (acetylating), wherein 4D is an acetyl-CoA carboxylase or a malonyl-CoA decarboxylase, wherein 4F is an oxaloacetate dehydrogenase or an oxaloacetate oxidoreductase, wherein 4G is a malonate semialdehyde dehydrogenase (acylating), wherein 4H is a pyruvate carboxylase, wherein 4J is a malonate semialdehyde dehydrogenase, wherein 4K is a malonyl-CoA synthetase or a malonyl-CoA transferase, wherein 4L is a malic enzyme, wherein 4M is a malate dehydrogenase or a malate oxidoreductase, wherein 4N is a pyruvate kinase or a PEP phosphatase, wherein 5A is a citrate synthase, wherein 5B is a citrate transporter, wherein 5C is a citrate/malate transporter, wherein 5D is an ATP citrate lyase, wherein 5E is a citrate lyase, wherein 5F is an acetyl-CoA synthetase or an acetyl-CoA transferase, wherein 5H is a cytosolic malate dehydrogenase, wherein 51 is a malate transporter, wherein 5J is a mitochondrial malate dehydrogenase, wherein 5K is an acetate kinase, wherein 5L is a phosphotransacetylase, wherein 6A is a citrate synthase, wherein 6B is a citrate transporter, wherein 6C is a citrate/oxaloacetate transporter, wherein 6D is an ATP citrate lyase, wherein 6E is a citrate lyase, wherein 6F is an acetyl-CoA synthetase or an acetyl-CoA transferase, wherein 6G is an oxaloacetate transporter, wherein 6K is an acetate kinase, and wherein 6L is a phosphotransacetylase, wherein 1T is a fructose-6-phosphate phosphoketolase, wherein 1U is a xylulose-5-phosphate phosphoketolase, wherein 1V is a phosphotransacetylase, wherein 1W is an acetate kinase, wherein 1X is an acetyl-CoA transferase, an acetyl-CoA synthetase, or an acetyl-CoA ligase.   
     
     
         79 . The non-naturally occurring microbial organism of  claim 78 , wherein said microbial organism comprises two, three, four, five, six, seven or eight exogenous nucleic acids each encoding an acetyl-CoA pathway enzyme. 
     
     
         80 . The non-naturally occurring microbial organism of  claim 79 , wherein said microbial organism comprises exogenous nucleic acids encoding each of the acetyl-CoA pathway enzymes of at least one of the pathways selected from (1)-(36). 
     
     
         81 . A method for producing isopropanol comprising culturing the non-naturally occurring microbial organism of any one of  claims 60 - 80  under conditions for a sufficient period of time to produce isopropanol. 
     
     
         82 . The method of  claim 81 , wherein said method further comprises separating the isopropanol from other components in the culture. 
     
     
         83 . The method of  claim 82 , wherein the separating comprises extraction, continuous liquid-liquid extraction, pervaporation, membrane filtration, membrane separation, reverse osmosis, electrodialysis, distillation, crystallization, centrifugation, extractive filtration, ion exchange chromatography, absorption chromatography, or ultrafiltration. 
     
     
         84 . Culture medium comprising bioderived isopropanol, wherein said culture medium is separated from a non-naturally occurring microbial organism of any one of  claims 60 - 80 . 
     
     
         85 . A bioderived isopropanol produced according to the method of any one of  claims 81 - 83 . 
     
     
         86 . The bioderived isopropanol of  claim 85 , wherein said bioderived compound has an Fm value of at least 80%, at least 85%, at least 90%, at least 95% or at least 98%. 
     
     
         87 . A composition comprising said bioderived isopropanol of  claim 85  or  86  and a compound other than said bioderived isopropanol. 
     
     
         88 . The composition of  claim 87  wherein said compound other than said bioderived compound is a trace amount of a cellular portion of a non-naturally occurring microbial organism having:
 (i) a formaldehyde fixation pathway; 
 (ii) a formate assimilation pathway; and/or 
 (iii) a methanol metabolic pathway, and 
 an isopropanol pathway. 
 
     
     
         89 . A composition comprising the bioderived isopropanol of  claim 85  or  86 , or a cell lysate or culture supernatant thereof. 
     
     
         90 . A biobased product comprising said bioderived compound of  claim 85  or  86 , wherein said biobased product is a solvent, a paint, lacquer, thinner, ink, adhesive, cleaner, disinfectant, cosmetic, toiletry, de-icer, pharmaceutical, motor oil, isopropylamine, isopropylether, isopropyl ester, propylene or a polymer. 
     
     
         91 . The biobased product of  claim 90  comprising at least 5%, at least 10%, at least 20%, at least 30%, at least 40% or at least 50% said bioderived compound. 
     
     
         92 . The biobased product of  claim 90  or  91 , wherein said biobased product comprises a portion of said bioderived compound as a repeating unit. 
     
     
         93 . A molded product obtained by molding a biobased product of any one of  claims 90 - 92 , wherein said biobased product is a polymer. 
     
     
         94 . A process for producing a biobased product of any one of  claims 90 - 92  comprising chemically reacting said bioderived compound with itself or another compound in a reaction that produces said biobased product. 
     
     
         95 . A non-naturally occurring microbial organism having:
 (i) a formaldehyde fixation pathway;   (ii) a formate assimilation pathway; and/or   (iii) a methanol metabolic pathway, and   a fatty acyl-ACP elongation (FAACPE) cycle in combination with a termination pathway, wherein said formaldehyde fixation pathway comprises:   (1) 1B and 1C; (2) 1D; or (3) 1D and 1Z,   wherein 1B is a 3-hexulose-6-phosphate synthase, wherein 1C is a 6-phospho-3-hexuloisomerase, wherein 1D is a dihydroxyacetone synthase, wherein 1Z is a fructose-6-phosphate aldolase,   wherein said formate assimilation pathway comprises a pathway selected from:   (4) 1E; (5) 1F, and 1G; (6) 1H, 1I, 1J, and 1K; (7) 1H, 1I, 1J, 1L, 1M, and 1N; (8) 1E, 1H, 1I, 1J, 1L, 1M, and 1N; (9) 1F, 1G, 1H, 1I, 1J, 1L, 1M, and 1N; (10) 1K, 1H, 1I, 1J, 1L, 1M, and 1N; and (11) 1H, 1I, 1J, 1O, and 1P,   wherein 1E is a formate reductase, 1F is a formate ligase, a formate transferase, or a formate synthetase, wherein 1G is a formyl-CoA reductase, wherein 1H is a formyltetrahydrofolate synthetase, wherein 1I is a methenyltetrahydrofolate cyclohydrolase, wherein 1J is a methylenetetrahydrofolate dehydrogenase, wherein 1K is a formaldehyde-forming enzyme or spontaneous, wherein 1L is a glycine cleavage system, wherein 1M is a serine hydroxymethyltransferase, wherein 1N is a serine deaminase, wherein 1O is a methylenetetrahydrofolate reductase, wherein 1P is an acetyl-CoA synthase,   wherein said methanol metabolic pathway comprises a pathway selected from:   (12) 10J; (13) 10A; (14) 10A and 10B; (15) 10A, 10B and 10C; (16) 10J, 10K and 10C; (17) 10J, 10M, and 10N; (18) 10J and 10L; (19) 10J, 10L, and 10G; (20) 10J, 10L, and 10I; (21) 10A, 10B, 10C, 10D, and 10E; (22) 10A, 10B, 10C, 10D, and 10F; (23) 10J, 10K, 10C, 10D, and 10E; (24) 10J, 10K, 10C, 10D, and 10F; (25) 10J, 10M, 10N, and 10O; (26) 10A, 10B, 10C, 10D, 10E, and 10G; (27) 10A, 10B, 10C, 10D, 10F, and 10G; (28) 10J, 10K, 10C, 10D, 10E, and 10G; (29) 10J, 10K, 10C, 10D, 10F, and 10G; (30) 10J, 10M, 10N, 10O, and 10G; (31) 10A, 10B, 10C, 10D, 10E, and 10I; (32) 10A, 10B, 10C, 10D, 10F, and 10I; (33) 10J, 10K, 10C, 10D, 10E, and 10I; (34) 10J, 10K, 10C, 10D, 10F, and 10I; and (35) 10J, 10M, 10N, 10O, and 10I,   wherein 10A is a methanol methyltransferase, wherein 10B is a methylenetetrahydrofolate reductase, wherein 10C is a methylenetetrahydrofolate dehydrogenase, wherein 10D is a methenyltetrahydrofolate cyclohydrolase, wherein 10E is a formyltetrahydrofolate deformylase, wherein 10F is a formyltetrahydrofolate synthetase, wherein 10G is a formate hydrogen lyase, wherein 10I is a formate dehydrogenase, wherein 10J is a methanol dehydrogenase, wherein 10K is a formaldehyde activating enzyme or spontaneous, wherein 10L is a formaldehyde dehydrogenase, wherein 10M is a S-(hydroxymethyl)glutathione synthase or spontaneous, wherein 10N is a glutathione-dependent formaldehyde dehydrogenase, wherein 10O is a S-formylglutathione hydrolase,   wherein said FAACPE cycle comprises one or more β-ketoacyl-ACP synthase, one or more β-ketoacyl-ACP reductase, one or more β-hydroxyacyl-ACP reductase, and one or more enoyl ACP-reductase,   wherein said termination pathway comprises a pathway selected from:   (36) 12I; (37) 12J; (38) 12I, 12K, and 12L; (39) 12I and 12O; (40) 12J and 12M; (41) 12I, 12K, 12L, and 12M; (42) 12I, 12O, and 12M; (43) 12I, 12K and 12N; and (44) 12P,   wherein 12I is a thioesterase, wherein 12J is a fatty acyl-ACP reductase, wherein 12K is an acyl-CoA synthase, wherein 12L is an acyl-CoA reductase, wherein 12M is a fatty aldehyde reductase, wherein 12N is a fatty alcohol forming acyl-CoA reductase (FAR), wherein 12O is a carboxylic acid reductase (CAR), wherein 12P is an acyl-ACP reductase (alcohol forming),   wherein an enzyme of the formaldehyde fixation pathway, the formate assimilation pathway, the methanol metabolic pathway, the FAACPE cycle or the termination pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce a compound of Formula (I):   
       
         
           
           
               
               
           
         
         wherein R 1  is C 1-24  linear alkyl; R 2  is CH 2 OH, CHO, or COOH; R 3  is H, OH, or oxo (═O); and   represents a single or double bond with the proviso that the valency of the carbon atom to which R 3  is attached is four, 
         wherein the substrate of each of said enzymes of the FAACPE cycle and the termination pathway are independently selected from a compound of Formula (II) or malonyl-ACP: 
       
       
         
           
           
               
               
           
         
         wherein R 1  is C 1-24  linear alkyl; R 3  is H, OH, or oxo (═O); R 4  is S-CoA, ACP, OH or H; and   represents a single or double bond with the proviso that the valency of the carbon atom to which R 3  is attached is four; 
         wherein said one or more enzymes of the FAACPE cycle are each selective for a compound of Formula (II) having a number of carbon atoms at R 1  that is no greater than the number of carbon atoms at R 1  of said compound of Formula (I), and 
         wherein said one or more enzymes of the termination pathway are each selective for a compound of Formula (II) having a number of carbon atoms at R 1  that is no less than the number of carbon atoms at R 1  of said compound of Formula (I). 
       
     
     
         96 . The non-naturally occurring microbial organism of  claim 95 , wherein said microbial organism has a formaldehyde fixation pathway and an FAACPE cycle in combination with a termination pathway. 
     
     
         97 . The non-naturally occurring microbial organism of  claim 95 , wherein said microbial organism has a formate assimilation pathway and an FAACPE cycle in combination with a termination pathway. 
     
     
         98 . The non-naturally occurring microbial organism of  claim 95 , wherein said microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, and an FAACPE cycle in combination with a termination pathway. 
     
     
         99 . The non-naturally occurring microbial organism of  claim 95 , wherein said microbial organism has a methanol metabolic pathway and an FAACPE cycle in combination with a termination pathway. 
     
     
         100 . The non-naturally occurring microbial organism of  claim 95 , wherein said microbial organism has a formaldehyde fixation pathway, a methanol metabolic pathway and an FAACPE cycle in combination with a termination pathway. 
     
     
         101 . The non-naturally occurring microbial organism of  claim 95 , wherein said microbial organism has a formate assimilation pathway, a methanol metabolic pathway and an FAACPE cycle in combination with a termination pathway. 
     
     
         102 . The non-naturally occurring microbial organism of  claim 95 , wherein said microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, a methanol metabolic pathway and an FAACPE cycle in combination with a termination pathway. 
     
     
         103 . The non-naturally occurring microbial organism of any one of  claims 95 - 102 , wherein the microbial organism further comprises an acetoacetyl-ACP pathway of (1) 12A, 12B, and 12C; or (2) 12A, 12B, and 12D,
 wherein 12A is an acetyl-CoA carboxylase, wherein 12B is malonyl-CoA ACP transacylase, wherein 12C is an acetoacetyl-ACP synthase, and wherein 12D is a β-ketoacyl-ACP synthase, or   wherein the microbial organism further comprises a 3-oxovalery-ACP pathway comprising an acetyl-CoA carboxylase, a malonyl-CoA ACP transacylase, and a β-ketoacyl-ACP synthase.   
     
     
         104 . The non-naturally occurring microbial organism of  103 , wherein an enzyme of the acetoacetyl-ACP pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce acetoacetyl-ACP, wherein an enzyme of the 3-oxovalery-ACP pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce 3-oxovalery-ACP, and wherein the acetoacetyl-ACP or the 3-oxovalery-ACP is a β-ketoacyl-ACP of the FAACPE cycle. 
     
     
         105 . The non-naturally occurring microbial organism of any one of  claims 95 - 104 , wherein R 1  is C 1-17  linear alkyl. 
     
     
         106 . The non-naturally occurring microbial organism of  claim 105 , wherein R 1  is C 9  linear alkyl, C 10  linear alkyl, C 11 , linear alkyl, C 12  linear alkyl or C 13  linear alkyl. 
     
     
         107 . The non-naturally occurring microbial organism of  claim 95 , wherein said microbial organism comprises two, three, or four exogenous nucleic acids each encoding an enzyme of said FAACPE cycle. 
     
     
         108 . The non-naturally occurring microbial organism of  claim 95 , wherein said microbial organism comprises two, three, or four exogenous nucleic acids each encoding an enzyme of said termination pathway. 
     
     
         109 . The non-naturally occurring microbial organism of  claim 95 , wherein said microbial organism comprises one, two, three, four, five, six, seven, or eight exogenous nucleic acids each encoding a formaldehyde fixation pathway enzyme, a formate assimilation pathway enzyme, or a methanol metabolic pathway enzyme. 
     
     
         110 . The non-naturally occurring microbial organism of  claim 95 , wherein said microbial organism comprises exogenous nucleic acids encoding each of the enzymes of at least one of the pathways selected from (1)-(44). 
     
     
         111 . The non-naturally occurring microbial organism of any one of  claims 95 - 110 , wherein said formate assimilation pathway further comprises:
 (1) 1Q; (2) 1R, and 1S; (3) 1Y and 1Q; (4) 1Y, 1R and 1S,   wherein 1Q is a pyruvate formate lyase, wherein 1R is a pyruvate dehydrogenase, a pyruvate ferredoxin oxidoreductase, or a pyruvate:NADP+ oxidoreductase, wherein 1S is a formate dehydrogenase, wherein 1Y is a glyceraldehyde-3-phosphate dehydrogenase or an enzyme of lower glycolysis.   
     
     
         112 . The non-naturally occurring microbial organism of any one of  claims 95 - 111 , wherein said organism further comprises a methanol oxidation pathway. 
     
     
         113 . The non-naturally occurring microbial organism of  claim 112 , wherein said organism comprises at least one exogenous nucleic acid encoding a methanol oxidation pathway enzyme expressed in a sufficient amount to produce formaldehyde in the presence of methanol, wherein said methanol oxidation pathway comprises 1A, wherein 1A a methanol dehydrogenase. 
     
     
         114 . The non-naturally occurring microbial organism of any one of  claims 95 - 113 , wherein said microbial organism further comprises 3H or 3P, wherein 3H is a hydrogenase, wherein 3P a carbon monoxide dehydrogenase. 
     
     
         115 . The non-naturally occurring micorobial organism of  claim 114 , wherein organism comprises an exogenous nucleic acid encoding said hydrogenase or said carbon monoxide dehydrogenase. 
     
     
         116 . The non-naturally occurring microbial organism of any one of  claims 95 - 115 , wherein said at least one exogenous nucleic acid encoding said formaldehyde fixation pathway enzyme, said formate assimilation pathway enzyme, said methanol metabolic pathway enzyme, said FAACPE cycle enzyme, said termination pathway enzyme, said acetoacetyl-ACP pathway enzyme, said 3-oxovalery-ACP pathway enzyme, said methanol oxidation pathway enzyme, said hydrogenase or said carbon monoxide dehydrogenase is a heterologous nucleic acid. 
     
     
         117 . The non-naturally occurring microbial organism of any one of  claims 95 - 116 , wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium. 
     
     
         118 . The non naturally occurring microbial organism of  claim 95 , wherein said enzyme of the formaldehyde fixation pathway, formate assimilation pathway, methanol metabolic pathway, FAACPE cycle or termination pathway is expressed in a sufficient amount to produce a compound selected from the Formulas (III)-(VI): 
       
         
           
           
               
               
           
         
         wherein R 1  is C 1-17  linear alkyl. 
       
     
     
         119 . The non-naturally occurring microbial organism of  claim 118 , wherein R 1  is C 9  linear alkyl, C 10  linear alkyl, C 11 , linear alkyl, C 12  linear alkyl or C 13  linear alkyl. 
     
     
         120 . The non-naturally occurring microbial organism of  claim 95 , wherein said microbial organism further comprises an acetyl-CoA pathway and at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme expressed in a sufficient amount to produce or enhance carbon flux through acetyl-CoA, wherein said acetyl-CoA pathway comprises a pathway selected from:
 (1) 3A and 3B; (2) 3A, 3C, and 3D; (3) 3H; (4) 3G and 3D; (5) 3E, 3F and 3B; (6) 3E and 3I; (7) 3J, 3F and 3B; (8) 3J and 3I; (9) 4A, 4B, and 4C; (10) 4A, 4B, 4J, 4K, and 4D; (11) 4A, 4B, 4G, and 4D; (12) 4A, 4F, and 4D; (13) 4N, 4H, 4B and 4C; (14) 4N, 4H, 4B, 4J, 4K, and 4D; (15) 4N, 4H, 4B, 4G, and 4D; (16) 4N, 4H, 4F, and 4D; (17) 4L, 4M, 4B and 4C; (18) 4L, 4M, 4B, 4J, 4K, and 4D; (19) 4L, 4M, 4B, 4G, and 4D; (20) 4L, 4M, 4F, and 4D; (21) 5A, 5B, 5D, 5H, 5I, and 5J; (22) 5A, 5B, 5E, 5F, 5H, 5I, and 5J; (23) 5A, 5B, 5E, 5K, 5L, 5H, 5I, and 5J; (24) 5A, 5C, 5D, 5H, and 5J; (25) 5A, 5C, 5E, 5F, 5H, and 5J; (26) 5A, 5C, 5E, 5K, 5L, 5H, and 5J; (27) 6A, 6B, 6D, and 6G; (28) 6A, 6B, 6E, 6F, and 6G; (29) 6A, 6B, 6E, 6K, 6L, and 6G; (30) 6A, 6C, and 6D; (31) 6A, 6C, 6E, and 6F; (32) 6A, 6C, 6E, 6K, and 6L; (33) 1T and 1V; (34) 1T, 1W, and 1X; (35) 1U and 1V; and (36) 1U, 1W, and 1X,   wherein 3A is a pyruvate oxidase (acetate-forming), wherein 3B is an acetyl-CoA synthetase, an acetyl-CoA ligase or an acetyl-CoA transferase, wherein 3C is an acetate kinase, wherein 3D is a phosphotransacetylase, wherein 3E is a pyruvate decarboxylase, wherein 3F is an acetaldehyde dehydrogenase, wherein 3G is a pyruvate oxidase (acetyl-phosphate forming), wherein 3H is a pyruvate dehydrogenase, a pyruvate:ferredoxin oxidoreductase, a pyruvate:NAD(P)H oxidoreductase or a pyruvate formate lyase, wherein 3I is an acetaldehyde dehydrogenase (acylating), wherein 3J is a threonine aldolase, wherein 4A is a phosphoenolpyruvate (PEP) carboxylase or a PEP carboxykinase, wherein 4B is an oxaloacetate decarboxylase, wherein 4C is a malonate semialdehyde dehydrogenase (acetylating), wherein 4D is an acetyl-CoA carboxylase or a malonyl-CoA decarboxylase, wherein 4F is an oxaloacetate dehydrogenase or an oxaloacetate oxidoreductase, wherein 4G is a malonate semialdehyde dehydrogenase (acylating), wherein 4H is a pyruvate carboxylase, wherein 4J is a malonate semialdehyde dehydrogenase, wherein 4K is a malonyl-CoA synthetase or a malonyl-CoA transferase, wherein 4L is a malic enzyme, wherein 4M is a malate dehydrogenase or a malate oxidoreductase, wherein 4N is a pyruvate kinase or a PEP phosphatase, wherein 5A is a citrate synthase, wherein 5B is a citrate transporter, wherein 5C is a citrate/malate transporter, wherein 5D is an ATP citrate lyase, wherein 5E is a citrate lyase, wherein 5F is an acetyl-CoA synthetase or an acetyl-CoA transferase, wherein 5H is a cytosolic malate dehydrogenase, wherein 5I is a malate transporter, wherein 5J is a mitochondrial malate dehydrogenase, wherein 5K is an acetate kinase, wherein 5L is a phosphotransacetylase, wherein 6A is a citrate synthase, wherein 6B is a citrate transporter, wherein 6C is a citrate/oxaloacetate transporter, wherein 6D is an ATP citrate lyase, wherein 6E is a citrate lyase, wherein 6F is an acetyl-CoA synthetase or an acetyl-CoA transferase, wherein 6G is an oxaloacetate transporter, wherein 6K is an acetate kinase, and wherein 6L is a phosphotransacetylase, wherein 1T is a fructose-6-phosphate phosphoketolase, wherein 1U is a xylulose-5-phosphate phosphoketolase, wherein 1V is a phosphotransacetylase, wherein 1W is an acetate kinase, wherein 1X is an acetyl-CoA transferase, an acetyl-CoA synthetase, or an acetyl-CoA ligase.   
     
     
         121 . The non-naturally occurring microbial organism of  claim 120 , wherein said microbial organism comprises two, three, four, five, six, seven or eight exogenous nucleic acids each encoding an acetyl-CoA pathway enzyme. 
     
     
         122 . The non-naturally occurring microbial organism of  claim 120 , wherein said microbial organism comprises exogenous nucleic acids encoding each of the acetyl-CoA pathway enzymes of at least one of the pathways selected from (1)-(36). 
     
     
         123 . The non-naturally occurring microbial organism of  claim 95 , further comprising one or more gene disruptions, said one or more gene disruptions occurring in endogenous genes encoding proteins or enzymes involved in: native production of ethanol, glycerol, acetate, formate, lactate, CO 2 , fatty acids, or malonyl-CoA by said microbial organism; transfer of pathway intermediates to cellular compartments other than the cytosol; or native degradation of a FAACPE cycle intermediate or a termination pathway intermediate by said microbial organism, wherein said one or more gene disruptions confer increased production of the compound of Formula (I) in said microbial organism. 
     
     
         124 . The non-naturally occurring microbial organism of  claim 123 , wherein said protein or enzyme is selected from the group consisting of a fatty acid synthase, an acetyl-CoA carboxylase, a biotin:apoenzyme ligase, an acyl carrier protein, a thioesterase, an acyltransferase, an ACP malonyltransferase, a fatty acid elongase, an acyl-CoA synthetase, an acyl-CoA transferase, an acyl-CoA hydrolase, a pyruvate decarboxylase, a lactate dehydrogenase, an alcohol dehydrogenase, an acid-forming aldehyde dehydrogenases, an acetate kinase, a phosphotransacetylase, a pyruvate oxidase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate phosphatase, a mitochondrial pyruvate carrier, a peroxisomal fatty acid transporter, a peroxisomal acyl-CoA transporter, a peroxisomal carnitine/acylcarnitine transferase, an acyl-CoA oxidase, and an acyl-CoA binding protein. 
     
     
         125 . The non-naturally occurring microbial organism of  claim 95 , wherein one or more enzymes of the FAACPE cycle or the termination pathway preferentially react with an NADH cofactor or have reduced preference for reacting with an NAD(P)H cofactor, wherein said one or more enzymes of the FAACPE cycle are a 3-ketoacyl-ACP reductase or an enoyl-ACP reductase, and wherein said one or more enzymes of the termination pathway are selected from an acyl-CoA reductase (aldehyde forming), an alcohol dehydrogenase, an acyl-CoA reductase (alcohol forming), a fatty acyl-ACP reductase, and a carboxylic acid reductase. 
     
     
         126 . The non-naturally occurring microbial organism of  claim 95 , further comprising one or more gene disruptions, said one or more gene disruptions occurring in genes encoding proteins or enzymes that result in an increased ratio of NAD(P)H to NAD(P) present in the cytosol of said microbial organism following said disruptions. 
     
     
         127 . The non-naturally occurring microbial organism of  claim 126 , wherein said gene encoding a protein or enzyme that results in an increased ratio of NAD(P)H to NAD(P) present in the cytosol of said microbial organism following said disruptions is selected from the group consisting of an NADH dehydrogenase, a cytochrome oxidase, a glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate phosphatase, an alcohol dehydrogenase, a pyruvate decarboxylase, an aldehyde dehydrogenase (acid forming), a lactate dehydrogenase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate:quinone oxidoreductase, a malic enzyme and a malate dehydrogenase. 
     
     
         128 . The non-naturally occurring organism of  claim 123  or  126 , wherein said one or more gene disruptions comprises a deletion of said one or more genes. 
     
     
         129 . The non-naturally occurring microbial organism of  claim 95 , wherein said microbial organism is Crabtree positive and is in culture medium comprising excess glucose, thereby increasing the ratio of NAD(P)H to NAD(P) present in the cytosol of said microbial organism. 
     
     
         130 . The non-naturally occurring microbial organism of  claim 95 , further comprising at least one exogenous nucleic acid encoding an extracellular transporter or an extracellular transport system for the compound of Formula (I). 
     
     
         131 . The non-naturally occurring microbial organism of  claim 95 , wherein one or more endogenous enzymes involved in: native production of ethanol, glycerol, acetate, formate, lactate, CO2, fatty acids, or malonyl-CoA by said microbial organism; transfer of pathway intermediates to cellular compartments other than the cytosol; or native degradation of a FAACPE cycle intermediate or a termination pathway intermediate by said microbial organism, has attenuated enzyme activity or expression levels. 
     
     
         132 . The non-naturally occurring microbial organism of  claim 131 , wherein said enzyme is selected from the group consisting of a fatty acid synthase, an acetyl-CoA carboxylase, a biotin:apoenzyme ligase, a thioesterase, an acyl carrier protein, a thioesterase, an acyltransferase, an ACP malonyltransferase, a fatty acid elongase, an acyl-CoA synthetase, an acyl-CoA transferase, an acyl-CoA hydrolase, a pyruvate decarboxylase, a lactate dehydrogenase, a short-chain alcohol dehydrogenase, an acid-forming aldehyde dehydrogenase, an acetate kinase, a phosphotransacetylase, a pyruvate oxidase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate phosphatase, a mitochondrial pyruvate carrier, a peroxisomal fatty acid transporter, a peroxisomal acyl-CoA transporter, a peroxisomal carnitine/acylcarnitine transferase, an acyl-CoA oxidase, and an acyl-CoA binding protein. 
     
     
         133 . The non-naturally occurring microbial organism of  claim 95 , wherein one or more endogenous enzymes involved in the oxidation of NAD(P)H or NADH, has attenuated enzyme activity or expression levels. 
     
     
         134 . The non-naturally occurring microbial organism of  claim 133 , wherein said one or more endogenous enzymes are selected from the group consisting of an NADH dehydrogenase, a cytochrome oxidase, a glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate phosphatase, an alcohol dehydrogenase, a pyruvate decarboxylase, an aldehyde dehydrogenase (acid forming), a lactate dehydrogenase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate:quinone oxidoreductase, a malic enzyme and a malate dehydrogenase. 
     
     
         135 . A method for producing a compound of Formula (I): 
       
         
           
           
               
               
           
         
         wherein R 1  is C 1-24  linear alkyl; R 2  is CH 2 OH, CHO, or COOH; R 3  is H, OH, or oxo (═O); and   represents a single or double bond with the proviso that the valency of the carbon atom to which R 3  is attached is four, comprising culturing the non-naturally occurring microbial organism of any one of  claims 95 - 134  under conditions for a sufficient period of time to produce said compound of Formula (I). 
       
     
     
         136 . The method of  claim 135 , wherein said method further comprises separating the compound of Formula (I) from other components in the culture. 
     
     
         137 . The method of  claim 136 , wherein the separating comprises extraction, continuous liquid-liquid extraction, pervaporation, membrane filtration, membrane separation, reverse osmosis, electrodialysis, distillation, crystallization, centrifugation, extractive filtration, ion exchange chromatography, absorption chromatography, or ultrafiltration. 
     
     
         138 . Culture medium comprising bioderived compound of Formula (I): 
       
         
           
           
               
               
           
         
         wherein R 1  is C 1-24  linear alkyl; R 2  is CH 2 OH, CHO, or COOH; R 3  is H, OH, or oxo (═O); and   represents a single or double bond with the proviso that the valency of the carbon atom to which R 3  is attached is four, wherein said culture medium is separated from a non-naturally occurring microbial organism of any one of  claims 95 - 134 . 
       
     
     
         139 . A bioderived compound of Formula (I): 
       
         
           
           
               
               
           
         
         wherein R 1  is C 1-24  linear alkyl; R 2  is CH 2 OH, CHO, or COOH; R 3  is H, OH, or oxo (═O); and   represents a single or double bond with the proviso that the valency of the carbon atom to which R 3  is attached is four, wherein said bioderived compound is produced according to the method of any one of  claims 135 - 137 . 
       
     
     
         140 . The bioderived compound of  claim 139 , wherein said bioderived compound has an Fm value of at least 80%, at least 85%, at least 90%, at least 95% or at least 98%. 
     
     
         141 . A composition comprising said bioderived compound of  claim 139  or  140  and a compound other than said bioderived compound. 
     
     
         142 . The composition of  claim 141 , wherein said compound other than said bioderived compound is a trace amount of a cellular portion of a non-naturally occurring microbial organism having:
 (i) a formaldehyde fixation pathway;   (ii) a formate assimilation pathway; and/or   (iii) a methanol metabolic pathway, and   a fatty acyl-ACP elongation (FAACPE) cycle in combination with a termination pathway.   
     
     
         143 . A composition comprising the bioderived compound of  claim 139  or  140 , or a cell lysate or culture supernatant thereof. 
     
     
         144 . A biobased product comprising said bioderived compound of  claim 139  or  140 , wherein said biobased product is a biofuel, chemical, polymer, surfactant, soap, detergent, shampoo, lubricating oil additive, fragrance, flavor material or acrylate. 
     
     
         145 . The biobased product of  claim 144  comprising at least 5%, at least 10%, at least 20%, at least 30%, at least 40% or at least 50% said bioderived compound. 
     
     
         146 . The biobased product of  claim 144  or  145 , wherein said biobased product comprises a portion of said bioderived compound as a repeating unit. 
     
     
         147 . A molded product obtained by molding a biobased product of any one of  claims 144 - 146 , wherein said biobased product is a polymer. 
     
     
         148 . A process for producing a biobased product of any one of  claims 144 - 146  comprising chemically reacting said bioderived compound with itself or another compound in a reaction that produces said biobased product. 
     
     
         149 . The non-naturally occurring microbial organism of any one of  claims 1 - 45  or any one of  claims 60 - 80  or any one of  claims 95 - 134 , wherein said acetyl-CoA pathway comprises 1T and 1V. 
     
     
         150 . The non-naturally occurring microbial organism of  claim 149 , wherein said formaldehyde fixation pathway comprises 1D and 1Z. 
     
     
         151 . The non-naturally occurring microbial organism of  claim 149  or  150 , wherein said formaldehyde fixation pathway comprises 1B and 1C. 
     
     
         152 . The non-naturally occurring microbial organism of any one of  claims 1 - 45 , wherein one or more enzymes of the MI-FAE cycle and/or MD-FAE cycle are each selective for a compound of Formula (II) wherein R 1  is C 9  linear alkyl, C 10  linear alkyl, C 11 , linear alkyl, C 12  linear alkyl or C 13  linear alkyl. 
     
     
         153 . The non-naturally occurring microbial organism of any one of  claims 1 - 45 , wherein one or more enzymes of the termination pathway are each selective for a compound of Formula (II) wherein R 1  is C 9  linear alkyl, C 10  linear alkyl, C 11 , linear alkyl, C 12  linear alkyl or C 13  linear alkyl. 
     
     
         154 . The non-naturally occurring microbial organism of any one of  claims 95 - 134 , wherein one or more enzymes of the FAACPE cycle are each selective for a compound of Formula (II) wherein R 1  is C 9  linear alkyl, C 10  linear alkyl, C 11 , linear alkyl, C 12  linear alkyl or C 13  linear alkyl. 
     
     
         155 . The non-naturally occurring microbial organism of any one of  claims 95 - 134 , wherein one or more enzymes of the termination pathway are each selective for a compound of Formula (II) wherein R 1  is C 9  linear alkyl, C 10  linear alkyl, C 11 , linear alkyl, C 12  linear alkyl or C 13  linear alkyl. 
     
     
         156 . The non-naturally occurring microbial organism of any one of  claims 1 - 45  or any one of  claims 60 - 80  or any one of  claims 95 - 134 , wherein said microbial organism further comprises attenuation of one or more endogenous enzymes selected from DHA kinase, methanol oxidase, PQQ-dependent methanol dehydrogenase, DHA synthase or any combination thereof. 
     
     
         157 . The non-naturally occurring microbial organism of  claim 156 , wherein said one or more endogenous enzymes is DHA kinase. 
     
     
         158 . The non-naturally occurring microbial organism of  claim 156 , wherein said one or more endogenous enzymes is methanol oxidase. 
     
     
         159 . The non-naturally occurring microbial organism of  claim 156 , wherein said one or more endogenous enzymes is PQQ-dependent methanol dehydrogenase. 
     
     
         160 . The non-naturally occurring microbial organism of  claim 156 , wherein said one or more endogenous enzymes is DHA synthase. 
     
     
         161 . The non-naturally occurring microbial organism of any one of  claims 1 - 45  or any one of  claims 60 - 80  or any one of  claims 95 - 134 , wherein said microbial organism further comprises attenuation of one or more endogenous enzymes of a competing formaldehyde assimilation or dissimilation pathway. 
     
     
         162 . The non-naturally occurring microbial organism of any one of  claims 1 - 45  or any one of  claims 60 - 80  or any one of  claims 95 - 134 , wherein said microbial organism further comprises a gene disruption of one or more endogenous nucleic acids encoding enzymes selected from DHA kinase, methanol oxidase, PQQ-dependent methanol dehydrogenase, DHA synthase or any combination thereof. 
     
     
         163 . The non-naturally occurring microbial organism of  claim 162 , wherein said gene disruption is of an endogenous nucleic acid encoding the enzyme DHA kinase. 
     
     
         164 . The non-naturally occurring microbial organism of  claim 162 , wherein said gene disruption is of an endogenous nucleic acid encoding the enzyme methanol oxidase. 
     
     
         165 . The non-naturally occurring microbial organism of  claim 162 , wherein said gene disruption is of an endogenous nucleic acid encoding the enzyme PQQ-dependent methanol dehydrogenase. 
     
     
         166 . The non-naturally occurring microbial organism of  claim 162 , wherein said gene disruption is of an endogenous nucleic acid encoding the enzyme DHA synthase. 
     
     
         167 . The non-naturally occurring microbial organism of any one of  claims 1 - 45  or any one of  claims 60 - 80  or any one of  claims 95 - 134 , wherein said microbial organism further comprises a gene disruption of one or more endogenous nucleic acids encoding enzymes of a competing formaldehyde assimilation or dissimilation pathway. 
     
     
         168 . A non-naturally occurring microbial organism having an acetyl-CoA pathway, wherein said acetyl-CoA pathway comprises a pathway selected from:
 (1) 1T and 1V; (2) 1T, 1W, and 1X; (3) 1U and 1V; (4) 1U, 1W, and 1X;   wherein 1T is a fructose-6-phosphate phosphoketolase, wherein 1U is a xylulose-5-phosphate phosphoketolase, wherein 1V is a phosphotransacetylase, wherein 1W is an acetate kinase, wherein 1X is an acetyl-CoA transferase, an acetyl-CoA synthetase, or an acetyl-CoA ligase,   wherein said non-naturally occurring microbial organism further comprises a pathway capable of producing isopropanol and an exogenous nucleic acid encoding an isopropanol pathway enzyme expressed in a sufficient amount to produce isopropanol, wherein said isopropanol pathway comprises a pathway selected from:   (1) 11V, 11W, 11X, and 11Y; or (2) 11T, 11U, 11W, 11X, and 11Y,   wherein 11T is an acetyl-CoA carboxylase, wherein 11U is an acetoacetyl-CoA synthase, wherein 11V is an acetyl-CoA:acetyl-CoA acyltransferase, wherein 11W is an acetoacetyl-CoA hydrolase, an acetoacetyl-CoA transferase, an acetoacetyl-CoA ligase, or a phosphotransacetoacetylase/acetoacetate kinase, wherein 11X is an acetoacetate decarboxylase, wherein 11Y is an acetone reductase or isopropanol dehydrogenase.